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HaloTag™ Technology: Cell Imaging and Protein Analysis
G. Los, Ph.D., R. Learish, Ph.D., Natasha Karassina, M.S., Chad
Zimprich, B.S., Mark G. McDougall, Ph.D., Lance P. Encell, Ph.D., Rachel
Friedman-Ohana, Ph.D., Monika Wood, M.S., Gediminas Vidugiris, Ph.D., Kris
Zimmerman, B.S., Paul Otto, M.S., Soshana Berstock, Ph.D., Dieter Klaubert,
Ph.D., and Ketih V. Wood, Ph.D., Promega Corporation and Promega Biosciences,
Inc.
The HaloTag™ Interchangeable Labeling Technology provides options
for rapid, site-specific labeling of proteins in living cells and in vitro. The
technology is based on the formation of a covalent bond between HaloTag™ Protein
and synthetic ligands that carry a variety of functionalities. The covalent bond
forms rapidly under physiological conditions, is highly specific and essentially
irreversible, yielding a complex that is stable even under denaturing
conditions.
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