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The MAO-Glo™ Assay: A Bioluminescent-Coupled Assay for Monoamine
Oxidase Activity
Michael P. Valley, Ph.D., Erika M. Hawkins, M.S., John Shultz,
Ph.D., Terry L. Riss, Ph.D., Keith V. Wood, Ph.D., Wenhui Zhou, Ph.D., Laurent
Bernad, Ph.D., Troy Good, Ph.D., Dave Good, B.S., and Dieter H. Klaubert, Ph.D.
Promega Corporation and Promega Biosciences, Inc.
Monoamine oxidases (MAOs) catalyze the oxidative deamination of
a number of biogenic and xenobiotic amines to the corresponding aldehydes with
consumption of O2 and production of H2O2, and are important in many drug
metabolism reactions. We have developed an assay that couples the activity of
MAO to the production of light by luciferase. The MAO-Glo™ Assay is a simple,
homogeneous and robust two-step bioluminescent-coupled assay for the rapid and
sensitive detection of MAO activity.
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