|
Download
article
200kb
pdf? |
|
Using Luciferase Assays to Study G-Protein Coupled Receptor
Pathways and Screen for GPCR Modulators
Frank Fan, Ph.D., Alieen Paguio, M.S., Denise Garvin, M.S., and
Keith V. Wood, Ph.D., Promega Corporation
Luciferase reporter assay systems are successfully used in the
drug discovery process against G-protein coupled receptors for both studying the
GPCR signaling pathways and for screening for modulators of GPCRs. Here we
describe a strategy for constructing stable cell lines expressing two
luciferases on two plasmids that reduces assay noise and false positives when
screening for downregulated responses (antagonist response of GPCR).
|
