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Assay Virtually Any Kinase With Kinase-Glo® Plus Luminescent
Kinase Assay, A Homogeneous High-Throughput Assay
Said Goueli, Ph.D. and Kevin Hsaio, M.S., Promega Corporation
Phosphotransferases represent a major group of cellular enzymes
that use ATP as a substrate and are implicated in a wide variety of cellular
functions including proliferation, differentiation and apoptosis. Their
substances encompass a wide range of chemical structures including proteins,
peptides, sugars and lipids. Because of the significance of these enzymes in
cellular functions, they have become valid targets for drug development. The
Kinase-Glo® Plus Assay can be used to monitor the activity of kinases and screen
for kinase inhibitors. This homogeneous, non-radioactive assay is robust and
amenable to high-throughput applications.
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