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Assay for DPPIV Activity Using a Homogeneous, Luminescent
Method
by Martha O'Brien, Ph.D.1, Bill Daily, Ph.D.2,
Mike Schurria, B.S.1, and Terry Riss, Ph.D.1, 1Promega
Corporation, 2Promega Biosciences
The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent
assay that measures activity of DPPIV, a serine protease that is involved in a
variety of biological processes including immune regulation, signal transduction
and apoptosis. In the assay, adding a single DPPIV-Glo™ Reagent results in DPPIV
cleavage of the supplied substrate and generation of a "glow-type" luminescent
signal that is proportional to DPPIV activity. The assay is easily amenable to
high-throughput screening applications.
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