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Miniaturizing and Automating Cell Viability and Reporter Assays
for High-Throughput and Ultrahigh-Throughput Screening
by Tracy Worzella, M.S., Brad Larson, B.A.,
Promega Corporation; Aoife Gallagher, Ph.D., Deerac Fluidics; and Eric Matthews,
BMG LABTECH
Screening facilities require simple solutions for measuring and
correlating a variety of parameters of cellular processes. Robust assays and
high-precision, low-volume, non-contact pipetting systems are now providing this
capability. We have demonstrated the scalability of the CellTiter-Glo®,
Caspase-Glo™ 3/7 and ChromaLuc™/ChromaGlo™ Technologies for high-throughput and
ultrahigh-througput cell-based screening in low-volume 384- and 1536-well
formats.
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