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Detecting Caspase Activity in Staurosporine-Treated Human
Neuroblastoma Cells Using Fluorescent and Luminescent Methods
Natalie Betz, Ph.D., Abigail Farfan, M.A., and Martha O'Brien,
Ph.D., Promega Corporation
We used the Apo-ONE® Homogeneous Caspase-3/7 Assay and the
Caspase-Glo™ 3/7 Assay to detect caspase-3/7 activity in human neuroblastoma
SH-SY5Y cells. Caspase-3/7 activity was measured at various time points after
adding assay reagents to SH-SY5Y cells treated with staurosporine. Optimal
sensitivity for detection of caspase activity was reached at 18 hours with the
Apo-ONE®
Homogeneous Caspase-3/7 Assay and at 1 hour with the Caspase-Glo™ 3/7 Assay.
Both assays proved to be sensitive methods for measuring caspace-3/7 activity in
SH-SY5Y neuroblastoma cells.
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