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Kinase-Glo™ Luminescent Kinase Assay: Detect Virtually
Any Kinase
by Richard Somberg, Ph.D., Beck Pferdehirt, and Kevin Kupcho,
B.S., Promega Corporation
The Kinase-Glo™ Luminescent Kianse Assay is a homogeneous method for
measuring kinase activity by quantifying a decrease in ATP levels following
kinase activity. The assay can work with virtually any kinase and substrate
combination, and it is performed in a single well of a standard multiwell plate
by adding an equal volume of Kinase-Glo™ Reagent and recording
luminescence. The assay produces excellent Z´-factor values, easily
detects known kinase inhibitors, and provides accurate IC50 values.
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