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Dual-Glo™ Luciferase Assay System: A Homogeneous
Dual-Reporter System
by Erika Hawkins, M.Sc., Braeden Butler, B.S., Keith Wood,
Ph.D., Michael O'Grady, M.S., Laurie Orr, B.S., and Michael Beck, M.S., Promega
Corporation
Promega has developed a new, homogeneous reporter assay, the Dual-Glo™
Luciferase Assay System, for monitoring both firefly and Renilla
luciferases expressed in mammalian cells in 96- and 384-well plates. The new
assay system integrates cell lysis and luminescence chemistry, and the
luminescence kinetics have been extended over several hours. The homogeneous
assay format allows dual-reporter assays to be more easily and rapidly performed
by reducing sample processing requirements and eliminating the need for reagent
injectors in luminometers.
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