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| 1. |
Wang, P.Y., Neretti, N., Whitaker, R., Hosier, S., Chang, C., Lu, D., Rogina, B., and Helfand, S.L.
(2009)
Long-lived Indy and calorie restriction interact to extend life span.
Proc. Natl. Acad. Sci. U S A
106(23)
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9262-7
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Notes:
These authors investigated the relationship between calorie restriction and INDY expression on lifespan in Drosophila melanogaster. They showed that calorie restriction downregulates INDY expression in normal flies. INDY mutants on a normal diet had increased lifespan that was not extended further by calorie restriction. The INDY long-lived flies also shared several phenotypic characteristics with normal flies on a calorie-restricted diet. The authors then demonstrated that the phenotypic effects of the INDY mutation were not caused by reduced ability to intake food. The results show that INDY and calorie restriction interact to extend lifespan, and that decreased INDY expression induces a calorie-restriction-like status. During the study, the Maxwell 16 Tissue DNA Purification System was used to isolate DNA from Drosophila. The DNA was used in PCR analyses to confirm the absence of Wolbachia DNA in the experimental lines.
(0004001) |
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Products: Maxwell® 16 Instrument | Maxwell® 16 Tissue LEV Total RNA Purification Kit |
| 2. |
Gaitanis, G., Velegraki, A., Alexopoulos, E.C., Kapsanaki-Gotsi, E., Zisova, L., Ran, Y., Zhang, H., Arsenis, G., Bassukas, I.D. and Faergemann, J.
(2009)
Malassezia furfur fingerprints as possible markers for human phylogeography.
ISME J.
3
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498–502
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Notes:
The authors examined Malassezia furfur strains isolated from Scandinavians permanently residing in Greece and clinical isolates from Greece, Bulgaria and China to determine if there was an association between strain and the host’s geographical origin and any underlying skin conditions. Prior to strain identification by PCR fingerprinting, M. furfur DNA was isolated using the Maxwell® 16 Instrument and a modified Maxwell® 16 Cell DNA Purification protocol. A single 2–3mm diameter yeast colony yielded 20ng of DNA.
(0003959) |
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Products: Maxwell® 16 Cell DNA Purification Kit |
| 3. |
Garm Spindler, K.L., Pallisgaard ,N., Rasmussen, A.A., Lindebjerg, J., Andersen, R.F., Crüger, D., and Jakobsen, A.
(2009)
The importance of KRAS mutations and EGF61A>G polymorphism to the effect of cetuximab and irinotecan in metastatic colorectal cancer.
Annals of Oncology
20
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879-884
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Notes:
These authors used the Maxwell® 16 System to isolate genomic DNA from whole blood and normal colonic tissue samples. The DNA was used in genotype analysis, testing for wildtype and mutant KRAS genes, and for various EGFR-related polymorphisms. The results were used in a research study testing the relationship between various genotypes and response to different treatment regimens.
(0003961) |
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Products: Maxwell® 16 Blood DNA Purification Kit | Maxwell® 16 Instrument | Maxwell® 16 Tissue DNA Purification Kit |
| 4. |
Leblanc, J.J., Brassinga, A.K., Ewann, F., Davidson, R.J. and Hoffman, P.S.
(2008)
An Ortholog of OxyR in Legionella pneumophila (LpOxyR) is expressed post-exponentially and negatively regulates the alkyl hydroperoxide reductase (ahpC2D) operon.
J. Bacteriol.
190
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3444-55
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Notes:
These authors used the Maxwell® 16 Total RNA Purification Kit and the Maxwell® 16 Instrument to purify total RNA from exponentially grown Legionella pneumophila for use in primer extension assays.
(0003798) |
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Products: Maxwell® 16 Instrument | Maxwell® 16 Total RNA Purification Kit |
| 5. |
Dahl Steffensen, K., Waldstrøm, M., Olsen, D.A., Corydon, T., Lorentzen, K.A., Hans Jørgen Knudsen, H.J., Jeppesen, U., Brandslund, I., and Jakobsen, A.
(2008)
Mutant epidermal growth factor receptor in benign, borderline, and malignant ovarian tumors.
Clin. Cancer Res.
14
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3278-3282
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Notes:
These authors evaluated 225 tumor samples from various ovarian and peritoneal tumor types for expression of the epidermal growth factor receptor type III variant EGFRvIII. EGFvIII has not been observed in normal tissues and so was evaluated as a potential target for tumor-specific therapies. However, none of the 225 samples evaluated were positive for this marker, suggesting that the EGFRvIII mutation is rare in ovarian tissue and is not a good therapeutic target for this disease. The authors used the Maxwell 16 Total RNA Purification Kit and the Maxwell 16 Instrument to purify RNA from tissue samples that had been fresh-frozen and fixed in the stabilization reagent RNAlater (Qiagen). Complete details of the RNA purification procedure are provided in the paper.
(0003878) |
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Products: Maxwell® 16 Instrument | Maxwell® 16 Total RNA Purification Kit |
| 6. |
Frederick, R.O., Bergeman, L., Blommel, P.G., Bailey, L.J., McCoy, J.G., Song, J., Meske, L., Bingman, C.A., Riters, M., Dillon, N.A., Kunert, J., Yoon, J.W., Lim, A., Cassidy, M., Bunge, J., Aceti, D.J., Primm, J.G., Markley, J.L., Phillips, G.N. Jr. and Fox, B.G.
(2008)
Small-scale, semi-automated purification of eukaryotic proteins for structure determination.
J. Struct. Funct. Genomics
8
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153-166
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Notes:
These authors describe a simple, small-scale screening method for recombinant polyhistidine-tagged proteins. They used the Maxwell® 16 Polyhistidine Protein Purification Kit and Maxwell® 16 Instrument to purify the proteins, and characterized the purified proteins by NMR and X-Ray analysis. They first used the Flexi® Vector System to clone the genes of interest into expression vectors containing either an N-terminal TEV protease cleavable His8-Maltose Binding Protein (His8-MBP) tag, or an in vivo cleaved His8-MBP tag. For small-scale screening, E. coli expressing fusion proteins were grown for 24 hours in auto-induction medium in 96-well growth blocks, harvested by centrifugation and resuspended to an OD600 of 20 in 1ml of 50mM HEPES (pH 7.5) containing a protease inhibitor cocktail. Aliquots of these cells were then added directly to the Maxwell® kit cartridge, and automated purification performed. The purified proteins were analyzed by SDS-PAGE or using a Caliper LC90 electrophoresis system. For purification of proteins for use in NMR or crystallography studies, 50ml overnight cultures were harvested and resuspended in 10 ml of 50mM HEPES (pH 7.5) containing 10 units of benzonase, 1mg/ml lysozyme, and protease inhibitor cocktail. The cell suspension was sonicated, and aliquots were then added to the Maxwell® 16 cartridge for purification. The authors purified 14 different proteins from humans, frogs, and zebrafish using this method. Detailed reports of yields obtained and subsequent analyses are provided in the paper.
(0003799) |
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Products: Maxwell® 16 Instrument | Maxwell® 16 Polyhistidine Protein Purification Kit |
| 7. |
Ansell, A., Farnebo, L., Grénman, R., Roberg, K., and Thunelm, L.K.
(2008)
Polymorphism of FGFR4 in cancer development and sensitivity to cisplatin and radiation in head and neck cancer.
Oral Oncology
doi:10.1016/j.oraloncology.2008.03.007
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Epub (ahead of print)
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Notes:
In this study, the Maxwell® 16 Instrument and Maxwell® 16 DNA Purification Kit were used to isolate DNA from cell lines derived from head and neck squamous cell carcinomas.
(0003891) |
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Products: Maxwell® 16 Cell DNA Purification Kit | Maxwell® 16 Instrument |
| 8. |
Suzuki, H., Morris, J.S., Li ,Y., Doll, M.A., Hein, D.W., Lium J., Jiao, L., Hassan, M.M., Day, R.S., Bondy, M.L., Abbruzzese, J.L., and Li, D.
(2008)
Interaction of the cytochrome P4501A2, SULT1A1 and NAT gene polymorphisms with smoking and dietary mutagen intake in modification of the risk of pancreatic cancer.
Carcinogenesis
29
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1184-1191
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Notes:
In this study, the Maxwell® 16 Instrument was used to purify genomic DNA from blood samples. The extracted DNA was amplified by PCR for subsequent genotype analysis.
(0003902) |
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Products: Maxwell® 16 Blood DNA Purification Kit | Maxwell® 16 Instrument |
| 9. |
Liu, P. Yeung, S.H.I., Crenshaw, K.A., Crouse, C.A., Scherer, J.R. and Mathies, R.A.
(2008)
Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer.
Forensic. Sci. Int: Genetics
2
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301–309
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Notes:
This paper describes analysis of DNA samples at a mock crime scene using automated DNA purification followed by STR analysis on a portable microchip system. The mock crime scene was created using "victim" and "suspect" blood stains prepared by spotting 3µl of liquid blood onto paper towels and a cloth shirt. The samples were allowed to dry overnight before placement at the crime scene. Samples were processed at the scene using the Maxwell® 16 Instrument and DNA IQ™ Casework Sample Kit for DNA extraction, and a microchip analyzer to perform amplification and STR analysis. The 9-plex autosomal STR typing system used in the microchip system included primer sequences from the PowerPlex® 16 System (D3S1358, THO1, D21S11, D5S818, D13S317, D7S820, vWA and D8S1179) and amelogenin for sex identification. DNA purification from suspect samples was completed in 2 hours, and subsequent STR analysis and generation of a "suspect" DNA profile took a further 3 hours. The entire process from sample collection to generation of a CODIS "hit" took 6 hours.
(0003922) |
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Products: 9947A DNA | 9948 Male DNA | DNA IQ™ Casework Sample Kit for Maxwell® 16 | Gold ST★R 10X Buffer | Maxwell® 16 Instrument |
| 10. |
Daniels R, Volkman SK, Milner DA, Mahesh N, Neafsey DE, Park DJ, Rosen D, Angelino E, Sabeti PC, Wirth DF, Wiegand RC.
(2008)
A general SNP-based molecular barcode for Plasmodium falciparum identification and tracking.
Malaria Journal
Oct 29:7
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223
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Notes:
These authors used the Maxwell® 16 System to isolate DNA from frozen whole blood samples infected with Plasmodium falciparum. The isolated DNA was used in a qPCR-based SNP genotyping assay that sought to uniquely identify the parasites based on their SNP marker profile.
(0003962) |
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Products: Maxwell® 16 Blood DNA Purification Kit | Maxwell® 16 Instrument |