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| 1. |
Munoz, J.R., Stoutenger, B.R., Robinson, A.P., Spees, J.L. and Prockrop, D.J.
(2005)
Human stem/progenitor cells from bone marrow promote neurogenesis of endogenous neural stem cells in the hippocampus of mice.
Proc. Natl. Acad. Sci. U S A
102
,
18171-18176
.
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Notes:
In this study, implantation of human bone marrow stem/progenitor cells (MSCs) into the hippocampus of immunodeficient mice was found to stimulate proliferation, migration and differentiation of endogenous neural stem cells. Results of immunohistochemical analyses were confirmed by ELISA. The NGF Emax® Immunoassay System was used to determine NGF levels in isolated hippocampi at various intervals after implantation of the MSCs.
(0003500) |
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Products: NGF Emax® ImmunoAssay System |
| 2. |
Wang, B., Jenkins, J.R., and Trayhurn, P.
(2005)
Expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture: integrated response to TNFα.
Am. J. Endocrinol. Metab.
288
,
E731-E740
.
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Notes:
In this study, the expression of various adipokine genes linked to inflammation was examined during differentiation of pre-adipocytes to adipocytes in primary culture. The effect of TNFα on expression of these adipokines in differentiated human adipocytes was also investigated. qPCR was used to measure expression levels of the various adipokines, including NGF, during development and upon TNFα exposure. In addition, secretion of adipokines into the culture media was measured by ELISA, the NGF Emax® ImmunoAssay System being used to determine NGF levels.
(0003502) |
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Products: NGF Emax® ImmunoAssay System |
| 3. |
McGough, N.N., He, D.Y., Logrip, M.L., Jeanblanc, J., Phamluong, K., Luong, K., Kharazia, V., Janak, P.H. and Ron D.
(2004)
RACK1 and brain-derived neurotrophic factor: a homeostatic pathway that regulates alcohol addiction.
J. Neurosci.
24
,
10542-10552
.
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Notes:
The authors examine the role of brain-derived neurotrophic factor (BDNF) in alcohol addition. RNA was isolated from primary rat hippocampal neurons cultured in the absence or presence of ethanol. The RNA was reverse transcribed using the Reverse Transcription System, and BDNF and GPDH RNAs were quantitated by fluorescent real-time PCR. BDNF and nerve growth factor (NGF) protein levels were monitored using the BNDF and NGF Emax® ImmunoAssay Systems.
(0003441) |
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Products: BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | Reverse Transcription System |
| 4. |
Cavaletti, G., Bogliun, G., Marzorati, L., Zincone, A., Piatti, M., Colombo, N., Franchi, D., La Presa, M.T., Lissoni, A., Buda, A., Fei, F., Cundari, S. and Zanna, C.
(2004)
Early predictors of peripheral neurotoxicity in cisplatin and paclitaxel combination chemotherapy.
Ann. Oncol.
15
,
1439–42
.
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Notes:
Patients with locally advanced squamous cervical carcinoma were monitored for chemotherapy-induced peripheral neurotoxicity (CIPN). Plasma drawn from the study participants was stored at –80°C prior to testing for nerve growth factor (NGF)levels using the NGF Emax® ImmunoAssay System. The authors note that a highly significant correlation existed between a decrease in circulating levels of NGF and an increase in the severity of CIPN.
(0003338) |
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Products: NGF Emax® ImmunoAssay System |
| 5. |
Guha, U., Gomes, W.A., Samanta, J., Gupta, M., Rice, F.L. and Kessler, J.A.
(2004)
Target-derived BMP signaling limits sensory neuron number and the extent of peripheral innervation in vivo.
Development
131
,
1175–86
.
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Notes:
To study the role of bone morphogenetic protein (BMP) in the development of peripheral sensory neurons, transgenic mice were created that overexpressed either a BMP inhibitor, noggin, or a ligand, BMP4. To determine if the increased trigeminal innervation and neuron numbers seen in these mice were due to increased neurotrophin levels, the mystacial (whisker) pads of 3-day-old mice were tested for the presence of NGF, BDNF and NT-3 using each of the corresponding Emax® ImmunoAssay Systems. The results indicated there were no significant changes in neurotrophin levels except for a decrease in NT-3 in the noggin transgenic mice.
(0003337) |
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Products: BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 6. |
Zhou, S., Chen, L.S., Miyauchi, Y., Miyauchi, M., Kar, S., Kangavari, S., Fishbein, M.C., Sharifi, B. and Chen, P.S.
(2003)
Mechanisms of cardiac nerve sprouting after myocardial infarction in dogs.
Circ. Res.
95
,
76–83
.
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Notes:
After inducing myocardial infarction in feral dogs, transcardiac NGF levels were measured in the serum from 0.5 hours to 1 month later. This difference in NGF concentration between the coronary sinus and aorta was measured in triplicate samples using the NGF Emax® ImmunoAssay System.
(0003336) |
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Products: NGF Emax® ImmunoAssay System |
| 7. |
Dorfman, M., Arancibia, S., Fiedler, J.L. and Lara, H.E.
(2003)
Chronic intermittent cold stress activates ovarian sympathetic nerves and modifies ovarian follicular development in the rat.
Biol. Reprod.
68
,
2038-2043
.
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Notes:
The authors studied the effects of a chronic intermittent cold stress regime on sympathetic nerve activation and ovarian physiology. To analyze whether this effect on noradrenaline was preceded by activation of the neurotrophic factor system responsible for growth and survival of sympathetic neurons, they measured nerve growth factor in adult female Sprague-Dawley rats using the NGF Emax® ImmunoAssay System.
(0002772) |
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Products: NGF Emax® ImmunoAssay System |
| 8. |
Grimm, J., Lu, L., Hayashi, T., Hope, B., Su, T. and Shaham, Y.
(2003)
Time-dependent increases in brain-derived neurotrophic factor protein levels within the mesolimbic dopamine system after withdrawal from cocaine: implications for incubation of cocaine craving.
J. Neurosci.
23
,
742-747
.
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Notes:
The BDNF Emax® ImmunoAssay System and the NGF Emax® ImmunoAssay System were used to assay the levels of neurotrophinin in male Long–Evans rat brains. After cocaine consumption, brain tissue was homogenized and the BDNF and NGF levels assayed.
(0002805) |
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Products: BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System |
| 9. |
Cavaliere, F., Sancesario, G., Bernardi, G. and Volonte, C.
(2002)
Extracellular ATP and nerve growth factor intensify hypoglycemia-induced cell death in primary neurons: role of P2 and NGFRp75 receptors.
J. Neurochem.
83
,
1129-1138
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Notes:
Cerebellar granule neurons were isolated from 8-day-old Wistar rats and were glucose deprived for 1 hour in Earl’s balanced salt solution. This solution was collected and assayed for NGF using the NGF Emax® ImmunoAssay System.
(0002809) |
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Products: NGF Emax® ImmunoAssay System |
| 10. |
Touhami, A., Grueterich, M. and Tseng, S.C.
(2002)
The role of NGF signaling in human limbal epithelium expanded by amniotic membrane culture.
Invest. Ophthalmol. Vis. Sci.
43
,
987-994
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Notes:
Human amniotic membrane, either intact or epithelially denuded, was homogenized and NGF content measured using the NGF Emax® ImmunoAssay System.
(0002807) |
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Products: NGF Emax® ImmunoAssay System |
| 11. |
Lee, P.G., Zhuo, H., and C.J. Helke
(2001)
Axotomy alters neurotrophin and neurotrophin receptor mRNAs in the vagus nerve and nodose ganglion of the rat.
Brain Res. Mol. Brain Res.
87
,
31-41
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Notes:
Protein and mRNA levels of various neurotrophins and neurotrophin receptors were analyzed in transected cervical vagus nerve and nodose ganglion. Nerve growth factor and neurotrophin-3 protein levels were measured using Promega's NGF Emax® ImmunoAssay System and NT-3 Emax® ImmunoAssay System, respectively.
(0002336) |
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Products: NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 12. |
Weis, C., Marksteiner, J. and Humpel, C.
(2001)
Nerve growth factor and glial cell line-derived neurotrophic factor restore the cholinergic neuronal phenotype in organotypic brain slices of the basal nucleus of Meynert.
Neuroscience
102
,
129-38
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Notes:
In Alzheimer's disease, cholinergic neurons are lost from the medial septum and nucleus basalis of Meynert. Nerve growth factor and glial cell line-derived neurotrophic factor were examined to determine if these growth factors can rescue the cholinergic neurons. GDNF and NGF levels in the sections of the basal nucleus of Meynert in rat was determined using Promega's GDNF Emax® ImmunoAssay System and NGF Emax® ImmunoAssay System, respectively. Glial cell line-derived neurotrophic factor was capable of partially restoring the number of cholinergic neurons.
(0002325) |
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Products: GDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System |
| 13. |
Das, K.P., Chao, S.L., White, L.D., Haines, W.T., Harry, G.J., Tilson, H.A., and Barone, S. Jr.
(2001)
Differential patterns of nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3 mRNA and protein levels in developing regions of rat brain.
Neuroscience
103
,
739-761
.
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Notes:
Regional and temporal patterns of neurotrophin messenger RNA and protein levels were characterized in the developing rat hippocampus, neocortex and cerebellum on postnatal days 1, 7, 14, 21, and 92. Brain-derived neurotrophic factor nerve growth factor, and neurotropin-3 protein levels were determined using Promega's BDNF Emax® ImmunoAssay System, NGF Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System.
(0002319) |
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Products: BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 14. |
Jezierski, M.K., and Sohrabji, F.
(2001)
Neurotrophin expression in the reproductively senescent forebrain is refractory to estrogen stimulation.
Neurobiol. Aging
22
,
309-319
.
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Notes:
The regulation of brain-derived neurotrophic factor, nerve growth factor and other neurotrophin ligands and receptors by estrogen was characterized in young adult and reproductively senescent rat brains. BNDF, NT-4, and NGF protein levels were monitored using Promega's BDNF Emax® ImmunoAssay, NT-4 Emax® ImmunoAssay System, and NGF Emax® ImmunoAssay Systems, respectively .
(0002320) |
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Products: BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System |
| 15. |
Woodhall E., West A.K., and Chuah M.I..
(2001)
Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors.
Brain Res. Mol. Brain Res.
88
,
203-213
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Notes:
Transplantation of olfactory ensheathing cells into lesions in the central nervous system is able to stimulate the growth of axons and in some cases restore functional connections. To investigate the mechanism, the authors quantitated the production of growth factors and expression of corresponding receptors by rat olfactory ensheathing cells. Levels of brain-derived neurotrophic factor, glial-cell-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 were monitored using Promega's BDNF Emax® ImmunoAssay System, GDNF Emax® ImmunoAssay System, NGF Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System, respectively.
(0002318) |
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Products: BDNF Emax® ImmunoAssay System | GDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 16. |
Zhu, Z.W., Friess, H., Wang, L., Bogardus, T., Korc, M., Kleeff, J., and Buchler M.W.
(2001)
Nerve growth factor exerts differential effects on the growth of human pancreatic cancer cells.
Clin. Cancer Res.
7
,
105-12
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Notes:
The levels of NGF in culture medium of numerous human pancreatic cancer cell lines was determined using Promega's NGF Emax® ImmunoAssay System. NGF-induced pancreatic cancer cell growth seemed to be dependent on the expression levels and the balance of the TrkA and p75 receptors.
(0002334) |
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Products: Anti-NGF mAb | NGF Emax® ImmunoAssay System |
| 17. |
Di Loreto, S., Corvetti, L., Maccarone, R., Piancatelli, D., and Adorno D.
(2000)
Interleukin 1-beta modulates the effects of hypoxia in neuronal culture.
J. Neuroimmunol.
106
,
32-42
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Notes:
Levels of nerve growth factor in untreated and IL-1beta hippocampal neuronal cultures were monitored during homeostasis, hypoxia and reoxygenation. Untreated cells exhibited a decrease in both the expression and release of NGF under mild hypoxic stress whereas IL-1beta treated cells exhibited an increase in both expression and release of NGF. NGF levels were quantitated using Promega's NGF Emax® ImmunoAssay System.
(0002332) |
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Products: NGF Emax® ImmunoAssay System |
| 18. |
Dissen, G.A., Parrott, J.A., Skinner, M.K., Hill, D.F., Costa, M.E. and Ojeda, S.R.
(2000)
Direct effects of nerve growth factor on thecal cells from antral ovarian follicles.
Endocrinology
141
,
4736-4750
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Notes:
Bovine thecal cells were grown for three days then subcultured for 24 hours. The culture media was then removed and NGF levels determined using the NGF Emax® ImmunoAssay System.
(0002810) |
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Products: NGF Emax® ImmunoAssay System |
| 19. |
Safieh-Garabedian, B., Dardenne, M., Kanaan, S.A., Atweh, S.F., Jabbur, S.J., and Saade, N.E.
(2000)
The role of cytokines and prostaglandin-E(2) in thymulin induced hyperalgesia.
Neuropharmacology
39
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1653-61
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Notes:
Thymulin induced thermal and mechanical hyperalgesia resulted in an increase in NGF, TNF alpha and PGE(2) levels in rat liver. NGF levels were determined with Promega's NGF Emax® ImmunoAssay System.
(0002335) |
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Products: NGF Emax® ImmunoAssay System |
| 20. |
Park, J.A., Lee, J.Y., Sato, T.A., and Koh, J.Y.
(2000)
Co-Induction of p75NTR and p75NTR-Associated Death Executor in Neurons After Zinc Exposure in Cortical Culture or Transient Ischemia in the Rat
J. Neurosci.
20
,
9096-9103
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Notes:
Mixed mouse cortical cultures containing both neurons and astrocytes were treated with zinc to induce neuronal death. Nerve growth factor augmented this zinc-induced neuronal death. The p75NTR receptor and the p75NTR-associated death executor (NADE) also play roles in zinc-triggered neuronal death. NGF levels were determined using Promega's NGF Emax® ImmunoAssay System.
(0002333) |
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Products: NGF Emax® ImmunoAssay System |
| 21. |
Dissen, G.A., Lara, H.E., Leyton, V., Paredes, A., Hill, D.F., Costa, M.E., Martinez-Serrano, A., and Ojeda, S.R.
(2000)
Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat
Endocrinology
141
,
1073-82
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Notes:
A rat model of human polycystic ovarian syndrome in which NGF-producing neural progenitor cells were grafted into ovaries of juvenile rats was used to study the effect of NGF levels in ovary tissue. NGF levels in conditional culture medium from immortalized embryonic day 16 rat hippocampus-derived neural progenitor cells and in whole rat ovaries was determined using Promega's NGF Emax® ImmunoAssay System.
(0002331) |
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Products: NGF Emax® ImmunoAssay System |
| 22. |
Xia, Y.X., Ikeda, T., Xia, X.Y., Ikenoue, T.
(2000)
Differential neurotrophin levels in cerebrospinal fluid and their changes during development in newborn rat.
Neurosci. Lett.
280
,
220-222.
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Notes:
The levels of BDNF, NT-3 and NGF were examined in the cerebrospinal fluid of newborn rats (80 per sample) from postnatal day 1-21. NGF levels were consistently higher than the other neurotrophins ranging from 136pg/ml (day 21) to 607pg/ml (day 17) depending upon the day tested. NT-3 was the next most abundant ranging from approximately 40pg/ml (day 19) to 144pg/ml (day 7). BDNF was the least abundant ranging from 19.2pg/ml (day 5) to as high as 65pg/ml (day 13). NGF was determined with the NGF Emax® ImmunoAssay System (NGF ELISA), NT-3 levels were determined with the NT-3 Emax® ImmunoAssay System (NT-3 ELISA), and BDNF levels were determined with the BDNF Emax® ImmunoAssay System (BDNF ELISA).
(0000167) |
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Products: Anti-NGF mAb | BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 23. |
Vizzard, M.A., Wu, K.H., Jewett, I.T.
(2000)
Developmental expression of urinary bladder neurotrophic factor mRNA and protein in the neonatal rat.
Brain Res. Dev. Brain Res.
119
,
217-224.
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Notes:
The amounts of neurotrophins NGF, NT-3 and NT-4 were examined in the bladders of Wistar rats at various times after birth up to adulthood. NGF ranged from 5ng per bladder (day 5) to10ng per bladder (day 15). NT-3 ranged from less than 1ng per bladder at postnatal day 30 to as high as about 18ng per bladder in adulthood. NT-4 ranged from about 1ng per bladder on day 30 to more than 40ng per bladder in adulthood. NGF, NT-3, and BDNF protein levels were determined with the NGF Emax® ImmunoAssay System, NT-3 Emax® ImmunoAssay System, and BDNF levels Emax® ImmunoAssay System, respectively. Extraction procedures are detailed.
(0000228) |
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Products: Anti-Human NT-3 pAb | BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 24. |
Heaton, M.B., Mitchell, J.J., Paiva, M., Walker, D.W.
(2000)
Ethanol-induced alterations in the expression of neurotrophic factors in the developing rat central nervous system.
Brain Res. Dev. Brain Res.
121
,
97-107
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Notes:
Brain-derived neurotrophic factor nerve growth factor, neurotrophin-3 levels were quantitated in neonatal and early postnatal rat hippocampus, septum, cortex/striatum and cerebellum using Promega's BDNF Emax® ImmunoAssay System, NGF Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System, respectively.
(0002312) |
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Products: Anti-Human BDNF pAb | BDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 25. |
Gottlieb, M., and Matute, C.
(1999)
Expression of nerve growth factor in astrocytes of the hippocampal CA1 area following transient forebrain ischemia.
Neuroscience
91
,
1027-1034
.
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Notes:
The NGF EMAX® ImmunoAssay System (NGF ELISA) was used to assay for NGF protein levels in the CA1 region of the hippocampus from rats experiencing a transient ischemia and control animals. References and detail of the tissue preparation is provided.
(0001113) |
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Products: NGF Emax® ImmunoAssay System |