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| 1. |
Luparello, C., Sirchia, R. and Pupello, D.
(2003)
PTHrP [67–86] regulates the expression of stress proteins in breast cancer cells inducing modifications in urokinase-plasminogen activator and MMP-1 expression.
J. Cell Sci.
116
,
2421-2430
.
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Notes:
Total and poly(A)+ RNA from treated and untreated 8701-BC breast cancer tumor cells were treated with RQ1 RNase-free DNase. The RNA samples were reverse transcribed using the M-MLV RNase H– point mutant reverse transcriptase and random primers to create cDNA used in downstream analyses. The cDNA from the reactions were used in PCR, differential display PCR, and semi-quantitative multiplex PCR reactions. In differential display PCR studies, the authors analyzed differentially displayed bands by staining 6% polyacrylamide gels with the SILVER SEQUENCE™ Staining Reagents. Diferentially displayed bands were re-amplified and cloned using the pGEM®-T Easy Vector and high efficiency JM109 competent cells.
(0003020) |
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Products: JM109 Competent Cells, >108cfu/μg | M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant | pGEM®-T Easy Vector System I | pGEM®-T Easy Vector System II | Random Primers | RQ1 RNase-Free DNase | SILVER SEQUENCE™ Staining Reagents |
| 2. |
Masibay, A., Mozer, T.J. and Sprecher, C.
(2000)
Promega Corporation reveals primer sequences in its testing kits.
J. Forensic Sci.
45(6)
,
1360-1362
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Notes:
This paper reveals the primer sequence for Promega's PowerPlex 1.1, amelogenin, FFFL and PowerPlex 1.2 Systems.
(0002265) |
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Products: Amelogenin (Fluorescein Detection) | Amelogenin (Silver Detection) | Amelogenin (TMR) | F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein) | PowerPlex® 1.1 System | PowerPlex® 1.2 System |
| 3. |
Chen, Z.D., Manchester, S.R., Sun, H.Y.
(1999)
Phylogeny and evolution of the Betulaceae as inferred from DNA sequences, morphology and paleobotany.
Am. J. Bot.
86
,
1168-1181
.
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Notes:
PCR products were purified with the Wizard® PCR Preps DNA Purification System and directly sequenced with the SILVER SEQUENCE™ DNA Sequencing System.
(0001334) |
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Products: SILVER SEQUENCE™ DNA Sequencing System | Wizard® PCR Preps DNA Purification System |
| 4. |
Jackson, A.U., Fornes, A., Galecki, A., Miller, R.A., Burke, D.T.
(1999)
Multiple-trait quantitative trait loci analysis using a large mouse sibship.
Genetics
151
,
785-795
.
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Notes:
20ng mouse genomic DNA was amplified with primers specific for 83 SSLP (single sequence length polymorphism) marker loci for genome-wide genotyping. Amplification products were separated on a denaturing gel and were visualized by silver staining using the SILVER SEQUENCE™ DNA Sequencing System.
(0000965) |
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Products: SILVER SEQUENCE™ DNA Sequencing System | SILVER SEQUENCE™ Staining Reagents |
| 5. |
Plasilova, M., Stoilov, I., Sarfarazi, M., Kadasi, L., Ferakova, E., Ferak, V.
(1999)
Identification of a single ancestral CYP1B1 mutation in Slovak gypsies (Roms) affected with primary congenital glaucoma.
J. Med. Genet.
36
,
290-294
.
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Notes:
The protocol for silver staining with the SILVERSEQUENCE™ DNA Sequencing System was followed for staining an SSCP gel. A permanent record of the silver stained gel was made with the Automatic Processor Compatible Film.
(0000537) |
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Products: Automatic Processor Compatible (APC) Film | SILVER SEQUENCE™ DNA Sequencing Reagents | SILVER SEQUENCE™ DNA Sequencing System |
| 6. |
Halim, A.-B., LeGros, L., Geller, A., Kotb, M.
(1999)
Expression and functional interaction of the catalytic and regulatory subunits of human methionine adenosyltransferase in mammalian cells.
J. Biol. Chem.
274
,
29720-29725
.
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Notes:
Subunits of the cDNA for methionine adenosyltransferase were amplified from a plasmid using Taq DNA Polymerase and directly cloned into the pTARGET™ Mammalian Expression T-Vector. The sequence of the cloned inserts were confirmed with the fmol® DNA Cycle Sequencing System. The constructs were transiently transfected into COS-1 cells with the TransFast™ Reagent at a 1:1 ratio. Excellent detail is provided for the transfection.
(0001094) |
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Products: fmol® DNA Cycle Sequencing System | pTARGET™ Mammalian Expression Vector System | TransFast™ Transfection Reagent |
| 7. |
Willett, C. S., Harrison, R. G.
(1999)
Insights into genome differentiation: pheromone-binding protein variation and population history in the European corn borer (Ostrinia nubilalis).
Genetics
153
,
1743-1751
.
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Notes:
In this paper, DNA visualized on a sequencing gel is stained with the SILVER SEQUENCE™ DNA Sequencing Systems, and PCR products are cloned into the pGEM®-T Vector.
(0000187) |
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Products: pGEM®-T Vector System I | pGEM®-T Vector System II | SILVER SEQUENCE™ DNA Sequencing Reagents | SILVER SEQUENCE™ DNA Sequencing System | SILVER SEQUENCE™ Staining Reagents |
| 8. |
Olsen, K.M., Schaal, B.A.
(1999)
Evidence on the origin of cassava: Phylogeography of Manihot esculenta
Proc. Natl. Acad. Sci. U S A
96
,
5586-5591
.
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Notes:
The fmol® DNA Cycle Sequencing System was used to sequence the PCR-amplified G3PDH alleles from a number of haplotypes of cassava.
(0000604) |
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Products: fmol® DNA Cycle Sequencing System |
| 9. |
Singh, A.K. and Shichi, H.
(1998)
A novel glutathione peroxidase in bovine eye: Sequence analysis, mRNA level, and translation.
J. Biol. Chem.
273
,
26171-26178
.
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Notes:
The glutathione peroxidase (GPX) cDNA was translated in vitro with the TNT® T7 Quick Coupled System and compared to partially purified GPX native protein. Both were recognized by an antibody to the c-terminus of the cloned sequence. Further evidence that the proteins were identical were obtained from the Ouchterlony double immunodiffusion assay. Clones of the GPX protein were sequenced with the fmol® DNA Sequencing System.
(0000368) |
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Products: fmol® DNA Cycle Sequencing System | TNT® T7 Quick Coupled Transcription/Translation System | TNT® T7 Quick Coupled Transcription/Translation System, Trial Size |
| 10. |
Gleeson, J.G., Allen, K.M., Fox, J.W., Lamperti, E.D., Berkovic, S., Scheffer, I., Cooper, E.C., Bobyns, W.B., Minnerath, S.R., Ross, M.E., Walsh, C.A.
(1998)
Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein.
Cell
92
,
63-72
.
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Notes:
The SILVER SEQUENCE™ Staining Reagents were used to stain single-strand conformation polymorphism (SSCP) reactions separated on a 6% polyacrylamide gel. Aberrant bands were excised and the DNA was eluted. The isolated DNA was reamplified and characterized by sequence analysis.
(0001106) |
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Products: SILVER SEQUENCE™ Staining Reagents |
| 11. |
Koopman, W.J.M., Guetta, E., van de Wiel, C.C.M., Vosman, B., van den Berg, R.G.
(1998)
Phylogenetic relationships among Lactuca (Asteraceae) species and related genera based on ITS-1 DNA sequences.
Am. J. Bot.
85
,
1517-1530
.
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Notes:
The SILVER SEQUENCE™ DNA Sequencing System was used to determine the nucleotide sequence of cloned internal transcribed spacers.
(0000881) |
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Products: SILVER SEQUENCE™ DNA Sequencing System |
| 12. |
Beaumelle, B., Taupiac, M.P., Lord, J.M. and Roberts, L.M.
(1997)
Ricin A chain can transport unfolded dihydrofolate reductase into the cytosol.
J. Biol. Chem.
272
,
22097-22102
.
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Notes:
The SILVER SEQUENCE™ DNA Sequencing System, Rabbit Reticulocyte Lysate System, Wizard® Plus Minipreps DNA Purification System and Taq DNA polymerase were used in this study.
(0001473) |
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Products: Rabbit Reticulocyte Lysate System, Nuclease Treated | SILVER SEQUENCE™ DNA Sequencing System | Wizard® Plus Minipreps DNA Purification System |
| 13. |
Rosenthal, J.J.C, Liu, T.I. and Gilly, W.F.
(1997)
A family of delayed rectifier Kv1 cDNAs showing cell type-specific expression in the squid stellate ganglion/giant fiber lobe complex.
J. Neurosci.
17
,
5070-5079
.
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Notes:
The Riboprobe® in vitro Transcription System was used to produce 32P-labeled RNA probes for RNase protection assays. The fmol® DNA Cycle Sequencing System was used to directly sequence PCR products with [33P]end-labeled primers.
(0001526) |
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Products: fmol® DNA Cycle Sequencing System | Riboprobe® System—SP6 | Riboprobe® System—T3 | Riboprobe® System—T7 |
| 14. |
Nepomuceno, R.R., Henschen Edman, A.H., Burgess, W.H., Tenner, A.J.
(1997)
cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro.
Immunity
6
,
119-129
.
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Notes:
RT-PCR was performed with degenerate primers and the resulting 110bp product was subcloned with the pGEM®-T Vector System. The 110bp fragment was used to screen a cDNA library and four positive plaques were identified. The lambda clones were sequenced with the fmol® DNA Cycle Sequencing System. To sequence across the entire lambda clone nested deletions were made with the Erase-a-Base® System.
(0000650) |
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Products: fmol® DNA Cycle Sequencing System | Erase-a-Base® System (minus vectors & bacterial strain) | pGEM®-T Vector System I | pGEM®-T Vector System II |
| 15. |
Coburn, C.M. and Bargmann, C.I.
(1996)
A putative cyclic nucleotide-gated channel is required for sensory development and function in C. elegans.
Neuron
17
,
695-706
.
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Notes:
The system was used to determine the nucleotide sequence of all clones.
(0001558) |
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Products: fmol® DNA Cycle Sequencing System |
| 16. |
Harris, R., Sabatelli, L.M. and Seeger, M.A.
(1996)
Guidance cues at the drosophila CNS midline: Identification and characterization of two drosophila netrin/UNC-6 homologs.
Neuron
17
,
217-228
.
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Notes:
The system was used to determine the nucleotide sequence of the netrin cDNA clones.
(0001560) |
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Products: fmol® DNA Cycle Sequencing System |
| 17. |
Garcia, M. A. , Vazquez, J. , Gimenez, C. , Valdivieso, F. , Zafra, F.
(1996)
Transcription factor AP-2 regulates human apolipoprotein E gene expression in astrocytoma cells.
J. Neurosci.
16
,
7550-7556
.
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| |
Notes:
The fmol® DNA Cycle Sequencing System was used to determined the sequence of cloned PCR products.
(0001140) |
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Products: fmol® DNA Cycle Sequencing System |