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| 1. |
Wilson, P.M., Fazzone, W., LaBonte, M.J., Lenz, H.J. and Ladner, R.D.
(2009)
Regulation of human dUTPase gene expression and p53-mediated transcriptional repression in response to oxaliplatin-induced DNA damage.
Nucleic Acids Res.
37
,
78–95
.
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Notes:
The authors examined the role of p53 in modulating dUTPase promoter activity. Base substitution mutations of Sp1- and E2F-binding sites in the dUTPase promoter were performed using the GeneEditor™ in vitro Site-Directed Mutagenesis System. Each mutant was confirmed by DNA sequencing. To determine growth inhibition, HCT116 human colon cancer cells were seeded in 96-well plates at 3 × 103 cells/well and treated with 5-fluorouracil (5-FU), fluorodeoxyuridine (FUdR), oxaliplatin or in combination. After 72 hours, the CellTiter® 96 AQueous One Solution was dispensed into each well and absorbance measured. RNA was isolated from HCT116 p53+/+ and HCT116 p53-/- cells. cDNA was reverse transcribed from 200ng total RNA followed by multiplex qPCR using the Plexor™ qPCR System to amplify dUTPase, thymidylate synthase and GAPDH, a housekeeping gene. The 1.2 kb region of the dUTPase promoter upstream of the transcriptional start site was amplified by PCR and the fragment cloned into the pGL3-Basic Vector. Truncated promoters were also generated by PCR and cloned into the same vector. Drosophila SL-2 cells and HCT116 cell lines were seeded in a 24-well plate and transfected with dUTPase pGL3 promoter constructs or with pCI-Neo:p53WT, pCI-Neo:p53MUT and the empty pCI-neo Mammalian Expression Vector; all transfections included the pRL-TK Vector at a ratio of 1:10. After six hours, the cells were incubated in either fresh medium or medium containing a cytotoxic agent at the appropriate concentration. Thirty hours later, the cells were lysed, quantitated by Western blotting and 20µl of lysate analyzed with the Dual-Luciferase® Reporter Assay System. Electrophoretic mobility shift analyses (EMSA) were performed using –64 to –91 of the dUTPase-nuclear isoform transcriptional start site in the Gel Shift Assay System.
(0004031) |
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Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay | CellTiter 96® AQueous One Solution Reagent | CellTiter 96® AQueous One Solution Reagent | Dual-Luciferase® Reporter 1000 Assay System | Dual-Luciferase® Reporter Assay System | Dual-Luciferase® Reporter Assay System 10-Pack | Gel Shift Assay Core System | Gel Shift Assay System | GeneEditor™ in vitro Site-Directed Mutagenesis System | pCI Mammalian Expression Vector | pGL3 Basic Vector | Plexor® qPCR System | pRL-TK Vector |
| 2. |
Yamada, K., Takahashi, M., Hoshino, Y., Takahashi, H., Ichiyama, K., Tanaka, T. and Okamoto, H.
(2009)
Construction of an infectious cDNA clone of hepatitis E virus strain JE03-1760F that can propagate efficiently in cultured cells.
J. Gen. Virol.
90
,
457–462
.
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Notes:
The authors created a full-length infectious cDNA clone of a genotype 3 hepatitis E virus (strain JE03-1760F) for use in cell culture. The full-length ORF2 sequence of the JE03-1760F genome was amplified and cloned into the pCI Mammalian Expression Vector. The construct was transfected into PLC/PRF/5 cells for 3 days then analyzed by Western blotting.
(0004029) |
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Products: pCI Mammalian Expression Vector |
| 3. |
Fields, M.A., Zheng, M., Wall, P., Oberg, S. and Atherton, S.S.
(2008)
Uniocular anterior chamber inoculation of a tumor necrosis factor alpha-expressing recombinant of herpes simplex virus type 1 results in more rapid destruction and increased viral replication in the retina of the uninoculated eye.
J. Virol.
82
,
5068–5078
.
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Notes:
Since tumor necrosis factor alpha (TNF-α) is known to have a protective effect on herpes simplex virus type 1 (HSV-1) infection in mouse eyes and brains, a recombinant virus constitutively expressing TNF-α was generated to see if it affected virus location and spread in the eyes and brain. Mouse TNF-α DNA was subcloned from one vector using EcoRI and placed into the pCI Mammalian Expression Vector downstream of the CMV immediate/early enhancer/promoter region. Then the CMV enhancer/promoter::TNF-α region was digested with BamHI and BglII to be placed in a third vector between the UL49 and UL50 genes of HSV-1. This construct and two controls were used to create recombinant HSV-1 particles that were then injected into the eyes of BALB/c mice.
(0003984) |
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Products: pCI Mammalian Expression Vector |
| 4. |
Ha, V.L., Bharti, S., Inoue, H., Vass, W.C., Campa, F., Nie, Z., de Gramont, A., Ward, Y. and Randazzo, P.A.
(2008)
ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion.
J. Biol. Chem.
283
,
14915–14926
.
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Notes:
The authors wanted to understand the role of ASAP3, an Arf GTPase-activating protein in cancer cells. FLAG- or hemagglutinin-tagged ASAP3 and GAP-deficient mutants of ASAP3 were cloned in the pCI Mammalian Expression Vector. The constructs were transfected into cells and used in immunofluorescence and Western blotting experiments.
(0003987) |
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Products: pCI Mammalian Expression Vector |
| 5. |
Schäfer, P., Cymerman, I.A., Bujnicki, J.M. and Meiss, G.
(2007)
Human lysosomal DNase IIα contains two requisite PLD-signature (HxK) motifs: evidence for a pseudodimeric structure of the active enzyme species.
Protein Sci.
16
,
82–91
.
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Notes:
The authors used biochemical and mutational analysis to characterize human lysosomal DNaseIIα. Native DNaseIIα and site-directed mutants were expressed as Flag-His6-DNaseIIα and HA-tagged DNaseIIα using expression constructs created with the pCI Vector. Protein was expressed by transiently transfecting HEK 293-T cells using the TransFast™ Transfection Reagent. Flag-His6-DNaseIIα was purified using the MagneHis™ Ni-Particles, and this purified protein was used in nuclease assays to monitor catalytic activity and in gel filtration experiments and coimmunoprecipitation assays with HA-DNaseIIα to determine whether the active enzyme is monomeric.
(0003786) |
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Products: MagneHis™ Ni-Particles | pCI Mammalian Expression Vector | TransFast™ Transfection Reagent |
| 6. |
Zhuang, H. and Matsunami, H.
(2007)
Synergism of accessory factors in functional expression of mammalian odorant receptors.
J. Biol. Chem.
282
,
15284–15293
.
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Notes:
To explore the role that accessory proteins may play in successfully expressing odorant receptors (ORs) on the cell surface of heterologous cells, the authors explored cotransfecting receptor-transporting proteins RTP1S, Ric8b and Gαolf with an N-terminally tagged OR to generate a functional and ligand-specific expression system. Three tags (Rho, FLAG or HA) were inserted into the NheI and EcoRI sites of the pCI Mammalian Expression Vector. Amplified OR orfs were cloned into the MluI and NotI sites of the tagged pCI Mammalian Expression Vector. The accessory proteins were subcloned into HA-pCI (RTP1S) or pCI (RTP1S, Ric8b, Hsc70t and Gαolf). For cotransfection, the N-terminal tagged OR vectors (0.8µg) and the assessory proteins (individually or in combination; 0.8µg) were transfected into HEK293T or Hana3A cells. The transfected cells were then subjected to immunocytochemistry, live-cell surface staining, permeabilized staining or FACS analysis. The Dual-Glo™ Luciferase Assay System was used to assess OR activation via CRE elements on a firefly luciferase vector. pRL-SV40 Vector was the internal control for cell viability and transfection efficiency. HEK293T or Hana3A cells were plated on 96-well plates, transfected with 1µg of CRE-Luc vector, 1µg of pRL-SV40 Vector, 5µg of OR and 1µg total for all accessory proteins (0.25µg each protein with pCI Mammalian Expression Vector to keep amount of plasmid constant). Twenty-four hours posttransfection, the medium was changed to CD293 chemically defined medium, incubated for 30 minutes at 37°C then replaced with 25µl of odorant solution in CD293 for a second incubation of 4 hours at 4°C. Then the reporter protein expression levels were measured by luminescence.
(0003687) |
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Products: Dual-Glo® Luciferase Assay System | pCI Mammalian Expression Vector | pRL-SV40 Vector |
| 7. |
Gubser, C., Goodbody, R., Ecker, A., Brady, G., O'Neill, L.A., Jacobs, N. and Smith, G.L.
(2007)
Camelpox virus encodes a schlafen-like protein that affects orthopoxvirus virulence.
J. Gen. Virol.
88
,
1667-1676
.
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Notes:
The authors wanted to examine how the Camelpox virus (CMLV) gene 176R (v-slfn) might affect orthopoxvirus virulence since it shares sequence similarity to murine schlafen (m-slfn) proteins that inhibit T cell and fibroblast growth. HeLa cells were transiently transfected with either pCI Mammalian Expression Vector alone or pCI Mammalian Expression Vector expressing v-slfn with or without a HA tag. Cellular localization of the protein was visualized using either α-v-slfn antiserum or α-HA mAb.
(0003757) |
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Products: pCI Mammalian Expression Vector |
| 8. |
Kaimori, J.Y., Nagasawa, Y., Menezes, L.F., Garcia-Gonzalez, M.A., Deng, J., Imai, E., Onuchic, L.F., Guay-Woodford, L.M. and Germino, G.G.
(2007)
Polyductin undergoes notch-like processing and regulated release from primary cilia.
Hum. Mol. Genet.
16
,
942-956
.
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Notes:
To understand the post-translational processing of PKDH1 protein, which has a role in human disease, a single full-length cDNA of human PKHD1 was assembled from three overlapping amplimers generated from a human kidney cDNA library. This PKHD1 cDNA was cloned into the pCI Mammalian Expression Vector, then N-terminal FLAG or EGFP tags and a C-terminal 7XMyc tag were added for detection. However, the expression level was too low, and an expression-enhancing beta-globin intronic sequence was added between the construct promoter and PKHD1 cDNA sequence. The pCI-PKHD1 construct was transfected into HEK 293 cells and the protein expression assessed using affinity tag capture and Western blotting.
(0003758) |
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Products: pCI Mammalian Expression Vector |
| 9. |
Teixeira, F.M., Teixeira, H.C., Ferreira, A.P., Rodrigues, M.F., Azevedo, V., Macedo, G.C. and Oliveira, S.C.
(2006)
DNA vaccine using Mycobacterium bovis Ag85B antigen induces partial protection against experimental infection in BALB/c mice.
Clin. Vaccine Immunol.
13
,
930–935
.
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Notes:
To test the feasibility of the Mycobacterium bovis Ag85B gene as a DNA vaccine, the gene was amplified by PCR, introducing EcoRI and XbaI restriction endonucleases onto the primers, digested with the restriction enzymes and ligated into the pCI Mammalian Expression Vector. After transformation, the constructs were isolated using the Wizard® Plus Minipreps DNA Purification System and analyzed using endonuclease digestion and DNA sequencing. To confirm production of a maltose binding protein (MBP)-Ag85B fusion protein in E. coli, the cell lysate was analyzed by Western blot, stained with a rabbit anti-MBP serum and secondary antibody Anti-Rabbit IgG (Fc), AP Conjugate (1:10,000 dilution) and developed with BCIP/NBT. BALB/c mice pretreated with 10mM cardiotoxin prior to intramuscular DNA immunization using construct pCI-Ag85B, administered at days 0, 15, 30, and 45. The immunization course was 50µl of DNA at a concentration of 1µg/µl in PBS with each animal receiving a total of 100µg of plasmid DNA. Antibody response was assessed at 15 days with an ELISA assay and cytokine presence tested two weeks after immunization using splenocytes for ELISA assays and ICC.
(0003552) |
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Products: Anti-Rabbit IgG (Fc), AP Conjugate | pCI Mammalian Expression Vector | Wizard® Plus Minipreps DNA Purification System |
| 10. |
Gat, O., Grosfeld, H., Ariel, N., Inbar, I., Zaide, G., Broder, Y., Zvi, A., Chitlaru, T., Altboum, Z., Stein, D., Cohen, S. and Shafferman, A.
(2006)
Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen.
Infect. Immun.
74
,
3987–4001
.
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Notes:
After bioinformatics analysis of the Bacillus anthracis genome for potential vaccine targets, the 197 ORFs were selected for amplification. Using the genomic DNA of B. anthracis strain Vollum, all PCR products were generated using a 5’ primer incorporating the T7 promoter, the Kozak sequence, unique restriction enzyme sites and a start codon and a 3’ primer with a stop codon and three more unique restriction sites. The amplicons were then in vitro transcribed and translated using the TNT® T7 Quick for PCR DNA system and radiolabeled with [35S]methionine. The products were analyzed by SDS-PAGE and autoradiography. To assess the immunoreactivity of the translated products, the proteins were immunoprecipitated using anti-B. anthracis antisera. The seropositive candidate amplified ORFs were cloned into the pCI Mammalian Expression Vector using compatible RE sites. These vectors were introduced into ICR mice using a Helios gene gun system (0.5µg plasmid DNA on 1µm gold particles) and the mice immunized in 2 week intervals.
(0003515) |
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Products: pCI Mammalian Expression Vector | TNT® T7 Quick for PCR DNA |
| 11. |
Schirmbeck, R., Riedl, P., Fissolo, N., Lemonnier, F.A., Bertoletti, A. and Reimann, J.
(2005)
Translation from cryptic reading frames of DNA vaccines generates an extended repertoire of immunogenic, MHC class I-restricted epitopes.
J. Immunol.
174
,
4647–4656
.
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Notes:
To analyze whether CD8+ T cell responses can be generated from gene products translated from another reading frame from the primary ORF encoded by DNA vaccines, various constructs were made using the pCI Mammalian Expression Vector. These inserts included the small hepatitis B surface antigen (HBsAg) and its 3’ untranslated sequence up to the hepatitis B virus (HBV) poly(A) sequence, which contains the sequence encoding the C-terminal Pol344–832 fragment; the 832 residue Pol protein, and the large HBsAg in an alternative reading frame and an unrelated in frame and out of frame OVA OVA18–385 fragment. In the case of the OVA fragment, the CMV promoter was removed and substituted with the SV40 promoter from pSI Mammalian Expression Vector. The immune response to both the primary and secondary gene products was determined using transient transfection of LMH cells followed by Western blotting or immunoprecipitation or counting CD8+ T cells from spleen cells.
(0003553) |
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Products: pCI Mammalian Expression Vector | pSI Mammalian Expression Vector |
| 12. |
Smith, C.C., Yu, Y.X., Kulka, M., and Aurelian, L.
(2000)
A Novel Human Gene Similar to the Protein Kinase (PK) Coding Domain of the Large Subunit of Herpes Simplex Virus Type 2 Ribonucleotide Reductase (ICP10) Codes for a Serine-Threonine PK and Is Expressed in Melanoma Cells
J. Biol. Chem.
275(33)
,
25690-25699
.
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Notes:
The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from 5 x 107 human G361 melanoma cells. The isolated DNA was used for Southern blotting. Probes were constructed with the DNA 5'-End Labeling System. The cDNA for the H11 protein was expressed in 293 HEK cells using the pCI Mammalian Expression Vector.
(0000075) |
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Products: DNA 5´ End-Labeling System | pCI Mammalian Expression Vector | Wizard® Genomic DNA Purification Kit |
| 13. |
Fu, C.A., Shen, M., Huang, B.C.B., Lasaga, J., Payan, D.G., Luo, Y.
(1999)
TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton.
J. Biol. Chem.
274
,
30729-30737
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Notes:
The pCI Mammalian Expression Vector was used for transient expression of the 150kDa TNIK protein in Phoenix-A cells, a 293 derivative.
(0001126) |
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Products: pCI Mammalian Expression Vector |
| 14. |
Apergis, G.A., Crawford, N., Ghosh, D., Steppan, C.M., Vorachek, W.R., Wen, P. and Locker, J.
(1998)
A novel nk-2-related transcription factor associated with human fetal liver and hepatocellular carcinoma.
J. Biol. Chem.
273
,
2917-2925
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Notes:
This paper describes use of the PolyATtract® mRNA Isolation System, pCI Mammalian Expression Vector, and TNT® Coupled Wheat Germ Extract System for RNA isolation, gene expression and gel-shifts assays (EMSA) respectively.
(0001477) |
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Products: pCI Mammalian Expression Vector | PolyATtract® mRNA Isolation System I (Refill for Z5200) | PolyATtract® mRNA Isolation System II with Magnetic Stand | PolyATtract® mRNA Isolation System III with Magnetic Stand | PolyATtract® mRNA Isolation System IV (Refill for Z5300) | TNT® SP6 Coupled Wheat Germ Extract System | TNT® T3 Coupled Wheat Germ Extract System | TNT® T7 Coupled Wheat Germ Extract System | TNT® T7/SP6 Coupled Wheat Germ Extract System | TNT® T7/T3 Coupled Wheat Germ Extract System |
| 15. |
De Angelis, D.A., Miesenböck, Zemelman, B.V., Rothman, J.E.
(1998)
PRIM; Proximity imaging of green fluorescent protein-tagged polypeptides
Proc. Natl. Acad. Sci. U S A
95
,
12312-12316
.
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Notes:
In this paper, various constructs of GFP-polypeptides were prepared in the pCI Mammalian Expression Vector. The constructs were expressed in HeLa cells.
(0001319) |
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Products: pCI Mammalian Expression Vector |
| 16. |
Memon, S.A., Hou, J., Moreno, M.B., Zacharchuk, C.M.
(1998)
Apoptosis induced by a chimeric Fas/FLICE receptor: lack of requirement for Fas- or FADD-binding proteins.
J. Immunol.
160
,
2046-2049
.
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Notes:
The pCI Mammalian Expression Vector was used to express a dominant negative version of FADD containing only the death domain.
(0000678) |
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Products: pCI Mammalian Expression Vector |
| 17. |
Demolombe, S., Baro, I., Pereon, Y., Bliek, J., Mohammad-Panah, R., Pollard, H., Morid, S., Mannens, M., Wilde, A., Barhanin, J., Charpentier, F., Escande, D.
(1998)
A dominant negative isoform of the long QT syndrome 1 gene product
J. Biol. Chem.
273
,
6837-6843
.
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Notes:
The pCI Mammalian Expression Vector was used to express human cardiac KvLQT1 isoform 1 and KvLQT1 isoform 2 in COS-7 cells. Transfected cells were used for electrophysiology studies.
(0001270) |
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Products: pCI Mammalian Expression Vector |
| 18. |
Duncan, R.R., Westwood, P.K., Boyd, A. and Ashley, R.H.
(1997)
Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum.
J. Biol. Chem.
272
,
23880-23886
.
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Notes:
The pCI Mammalian Expression Vector was used to express the p64H1 protein in 293 cells and HT-4, a clonal neuronal cell line. Inserts in the vectors were verified for expression using the TNT® Coupled Reticulocyte Lysate System. The proteins were translated in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The pGEM®-T Vector System was used to clone the PCR product of circle rapid amplification of cDNA ends to find the true 5'-end of the p64H1 cDNA.The Tfx™-20 Reagent was used to transfect 293 cells and HT-4 neuronal cells with the p64H1-expressing pCI-based plasmid.
(0001206) |
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Products: pCI Mammalian Expression Vector | pGEM®-T Vector System I | pGEM®-T Vector System II | TNT® SP6 Coupled Reticulocyte Lysate System | TNT® SP6 Coupled Reticulocyte Lysate System, Trial Size | TNT® T3 Coupled Reticulocyte Lysate System | TNT® T7 Coupled Reticulocyte Lysate System | TNT® T7 Coupled Reticulocyte Lysate System, Trial Size | TNT® T7/SP6 Coupled Reticulocyte Lysate System | TNT® T7/T3 Coupled Reticulocyte Lysate System | Tfx™-20 Reagent |
| 19. |
Kelm, R. J., Jr., Elder, P. K., Strauch, A.R., Getz, M.J.
(1997)
Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Purα and Purβ.
J. Biol. Chem.
272
,
26727-26733
.
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Notes:
Single-stranded 3´-end biotinylated oligonucleotides were captured on the Streptavidin MagneSphere® Paramagnetic Particles (SA-PMPs) and incubated with heparin-agarose affinity-purified nuclear proteins. The reaction was performed under the conditions used for electrophoretic gel shifts. Proteins were eluted in 1X nonreducing SDS-PAGE sample buffer at 65°C for 3 minutes and analyzed by Southwestern blotting with 32P-labeled oligonucleotides. The ssDNA-affinity capture worked with the oligonucleotides prebound to the particles or after allowing the interactions to occur in solution prior to capture. The pCI Mammalian Expression Vector was used to express the proteins Purα (321 amino acids) and Purβ (324 amino acids) in mouse embryo-derived AKR-2B fibroblasts. Cell extracts were prepared and assayed by Southwestern blotting. The same proteins were in vitro translated with the TNT® Coupled Reticulocyte Lysate System and assayed as well.
(0000929) |
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Products: pCI Mammalian Expression Vector | Streptavidin MagneSphere® Paramagnetic Particles | TNT® SP6 Coupled Reticulocyte Lysate System | TNT® SP6 Coupled Reticulocyte Lysate System, Trial Size | TNT® T3 Coupled Reticulocyte Lysate System | TNT® T7 Coupled Reticulocyte Lysate System | TNT® T7 Coupled Reticulocyte Lysate System, Trial Size | TNT® T7/SP6 Coupled Reticulocyte Lysate System | TNT® T7/T3 Coupled Reticulocyte Lysate System |
| 20. |
Kelm, R.J. Jr., Elder, P.K., Strauch, A.R. and Getz, M.J.
(1997)
Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Pura and Purb.
J. Biol. Chem.
272(42)
,
26727-26733
.
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Notes:
Single-stranded 3'-end biotinylated oligonucleotides were captured on the paramagnetic particles and incubated with heparin-agarose affinity-purified nuclear proteins. The reaction was performed under the conditions used for electrophoretic gel shifts. Proteins were eluted in 1X non-reducing SDS-PAGE sample buffer at 65°C for 3 min and analyzed by Southwestern blotting with 32P-labeled oligonucleotides. The ssDNA-affinity capture apparently worked with the oligonucleotides prebound to the particles or after allowing the interactions to occur in solution prior to capture.
(0001666) |
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Products: pCI Mammalian Expression Vector | Streptavidin MagneSphere® Paramagnetic Particles |
| 21. |
Smith, C. L., Htun, H., Wolford, R. G , Hager, G. L.
(1997)
Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure.
J. Biol. Chem.
272
,
14227-14235
.
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Notes:
Several proteins were transiently expressed in the 1471.1 cell line using the pCI Mammalian Expression Vector.
(0000379) |
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Products: pCI Mammalian Expression Vector | pCI-neo Mammalian Expression Vector |
| 22. |
Salinas, M., Duprat, F., Heurteaux, C., Hugnot, J.P., Lazdunski, M.
(1997)
New Modulatory alpha Subunits for Mammalian Shab K+ Channels
J. Biol. Chem.
272
,
24371-24379
.
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Notes:
The pCI Mammalian Expression Vector was used to express a flag-tagged 497 amino acid potassium channel in COS cells.
(0000433) |
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Products: pCI Mammalian Expression Vector | pCI-neo Mammalian Expression Vector |
| 23. |
Postigo, A.A., Dean, D.C.
(1997)
ZEB, a vertebrate homolog of Drosophila Zfh-1, is a negative regulator of muscle differentiation.
EMBO J.
16
,
3935-3943
.
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Notes:
Both ZEB and the DNA-binding domain of ZEB were subcloned into Promega's pCI-neo Mammalian Expression Vector just behind a FLAG epitope. After cotransfection with reporter vectors, the effect of the expressed protein was assessed in 10T1/2 fibroblasts and C2C12 cells. The two protein inserts were subcloned into a T3 RNA polymerase binding site bearing plasmid and the flag-tagged proteins were expressed in vitro with the TNT® Coupled Reticulocyte Lysate System.
(0000542) |
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Products: pCI Mammalian Expression Vector | pCI-neo Mammalian Expression Vector | TNT® T3 Coupled Reticulocyte Lysate System |
| 24. |
Grolz, D. , Laubinger, J. , Wilmer, F. , Troster, H. , Bachmann, M.
(1997)
Transfection analysis of expression of mRNA isoforms encoding the nuclear autoantigen La/SS-B.
J. Biol. Chem.
272
,
12076-12082
.
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Notes:
Both pCI and pCI-neo Mammalian Expression Vectors were used to express proteins in Mouse LTA and NIH3T3 cells and human HeLa cells. The authors report, 'In general we observed that even after optimization of the transfection conditions the expression level of the pCI-neo constructs was only 25% compared with pCI constructs.'
(0001122) |
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Products: pCI Mammalian Expression Vector | pCI-neo Mammalian Expression Vector |
| 25. |
Chouabe, C. , Neyroud, N. , Guicheney, P. , Lazdunski, M. , Romey, G. , Barhanin, J.
(1997)
Properties of KvLQT1 K+ channel mutations in Romano-Ward and Jervell and Lange-Nielsen inherited cardiac arrhythmias
EMBO J.
16
,
5472-5479.
.
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Notes:
The 676 amino acid KvLQT1 protein and site-directed mutants were cloned into the pCI Mammalian Expression Vector, expressed in COS cells and assayed for activity.
(0001297) |
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Products: pCI Mammalian Expression Vector | pCI-neo Mammalian Expression Vector |