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| 1. |
Rogaev, E.I., Grigorenko, A.P., Moliaka, Y.K., Faskhutdinova, G., Goltsov, A., Lahti, A., Hildebrandt, C., Kittler, E.L. and Morozova, I.
(2009)
Genomic identification in the historical case of the Nicholas II royal family.
Proc. Natl. Acad. Sci. USA
106
,
5258–63
.
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Notes:
In 1991, the remains of murdered Emperor Nicholas II, Empress Alexandra and three of their five children were discovered. Until recently, the remains of the two other children were never found. In July of 2007 human bone fragments were discovered at a second grave site in the Ural region of Russia. The authors performed DNA typing to determine if these remains were those of the two missing children. Bone fragments and teeth were subjected to mitochondrial and nuclear DNA typing. DNA was quantitated using the Plexor® HY System. Nuclear DNA analysis was performed, in part, using the PowerPlex® S5 System. A comparison of mitochondrial DNA sequences from remains in the first and second graves and from maternal reference samples confirmed that the remains constituted a family with a "Queen Victoria" mitotype (Empress Alexandra was the granddaughter of Queen Victoria). Y-STR analysis of both sets of remains was performed, and the results confirmed that the Y-STR haplotypes of the two sets of male remains matched, and this haplotype matched that of several descendants from an unbroken paternal lineage of Nicholas I, father of Nichloas II. The mitochondrial and Y-STR haplotypes and autosomal STR profile also matched those obtained from a bloodstained shirt that Nicholas II was wearing during an assassination attempt.
(0003967) |
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Products: Plexor® HY System |
| 2. |
Yunis, J.J., García, O., Moreno, S., Pineda, C., Rodriguez, C., Uriarte, I. and Yunis, E.J.
(2008)
Population data on Powerplex 2.1 (FGA, vWA, TPOX, THO1, Penta E, D18S51, D21S11, D3S1358, D8S1179) and Gammastar (D16S539, D7S820, D13S317, D5S818) in a sample of Caucasian-Mestizos from Colombia.
Int. Congr. Ser.
1239
,
207–12
.
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Notes:
The authors generated population data for unrelated Caucasian-Mestizos from Columbia using the PowerPlex® 2.1 System and the GammaSTR® Multiplex. DNA was isolated from whole blood using the Wizard® Genomic DNA Purification Kit or ReadyAmp™ Genomic DNA Purification System, then amplified per the manufacturer's recommendations. Amplified fragments were detected using a Hitachi FMBIO® II fluorescence imaging system.
(0003856) |
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Products: GammaSTR® Multiplex (Fluorescein) D16S539, D7S820, D13S317, D5S818 | PowerPlex® 2.1 System | ReadyAmp™ Genomic DNA Purification System | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 3. |
French, A.J., Adams, C.A., Anderson, L.S., Kitchen, J.R., Hughes, M.R. and Wood, S.H.
(2008)
Development of human cloned blastocysts following somatic cell nuclear transfer with adult fibroblasts
Stem Cells
2008
,
485-493
.
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Notes:
Somatic cell nuclear transfer technique was used to generate human blastocyst-stage embryos using nuclei from adult male fibroblasts cell lines and enucleated oocytes. Genomic DNA was analyzed using the PowerPlex® 16 system to confirm the genetic identity of the blastocyst cells.
(0003952) |
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Products: PowerPlex® 16 System |
| 4. |
Marjanovic, D., Pojskic, N., Bakal, N., Drobnic, K.,Primorac, D., Bajrovic, K. and Hadziselmovic, R.
(2008)
Preliminary population study at fifteen autosomal and twelve Y-chromosome short tandem repeat loci in the representative sample of multinational Bosnia and Herzegovina residents.
Int. Congr. Ser.
1288
,
243–5
.
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Notes:
The authors generated population data for 100 unrelated individuals from three main ethnic groups in Bosnia and Herzegovina. Autosomal STR analysis was performed for 100 male and female individuals, and Y-STR analysis was performed with 100 male individuals. DNA was collected as buccal swabs or blood samples, isolated and amplified, and amplification products were detected using an ABI PRISM® 377 DNA Sequencer.
(0003877) |
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Products: PowerPlex® 16 System |
| 5. |
Krenke, B.E., Nassif, N., Sprecher, C.J., Knox, C., Schwandt, M. and Storts, D.R.
(2008)
Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA.
Forensic Sci. Int. Genet.
3
,
14–21
.
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Notes:
The authors describe the developmental validation of the Plexor® HY System, a quantitative PCR assay that simultaneously quantifies total human and male DNA. Validation studies examined: (1) human specificity, (2) sensitivity, (3) quantitation of degraded DNA, (4) impact of inhibitors, (5) male/female mixture and Y-assay male specificity, (6) reproducibility and concordance and (7) population studies.
(0003969) |
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Products: Plexor® HY System |
| 6. |
Liu, P. Yeung, S.H.I., Crenshaw, K.A., Crouse, C.A., Scherer, J.R. and Mathies, R.A.
(2008)
Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer.
Forensic. Sci. Int: Genetics
2
,
301–309
.
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Notes:
This paper describes analysis of DNA samples at a mock crime scene using automated DNA purification followed by STR analysis on a portable microchip system. The mock crime scene was created using "victim" and "suspect" blood stains prepared by spotting 3µl of liquid blood onto paper towels and a cloth shirt. The samples were allowed to dry overnight before placement at the crime scene. Samples were processed at the scene using the Maxwell® 16 Instrument and DNA IQ™ Casework Sample Kit for DNA extraction, and a microchip analyzer to perform amplification and STR analysis. The 9-plex autosomal STR typing system used in the microchip system included primer sequences from the PowerPlex® 16 System (D3S1358, THO1, D21S11, D5S818, D13S317, D7S820, vWA and D8S1179) and amelogenin for sex identification. DNA purification from suspect samples was completed in 2 hours, and subsequent STR analysis and generation of a "suspect" DNA profile took a further 3 hours. The entire process from sample collection to generation of a CODIS "hit" took 6 hours.
(0003922) |
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Products: 9947A DNA | 9948 Male DNA | DNA IQ™ Casework Sample Kit for Maxwell® 16 | Gold ST★R 10X Buffer | Maxwell® 16 Instrument |
| 7. |
McManus, B.A., Coleman, D.C., Moran, G., Pinjon, E., Diogo, D., Bougnoux, M.E., Borecká-Melkusova, S., Bujdákova, H., Murphy, P., d'Enfert, C. and Sullivan, D.J.
(2008)
Multilocus sequence typing reveals that the population structure of Candida dubliniensis is significantly less divergent than that of Candida albicans.
J. Clin. Microbiol.
46
,
652–64
.
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Notes:
To determine the usefulness of multilocus sequence typing (MLST) in differentiating Candida species during epidemiological studies, the authors investigated the population structure of C. dubliniensis by amplifying the same 10 MLST loci found to be useful in differentiating isolates of C. albicans, a closely related species. PCRs were performed using 1.25 units of GoTaq® Flexi DNA Polymerase and 1ng of DNA template in a 50µl reaction.
(0003880) |
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Products: GoTaq® Flexi DNA Polymerase |
| 8. |
Miozzo, M.C., Maxzud, M.K., Pacharoni, C.M., Mutal, S.A. and Modesti, N.M.
(2007)
Characterization of the variant allele 9.2 of Penta D locus.
J. Forensic Sci.
52
,
1073–6
.
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Notes:
During casework analysis using the PowerPlex® 16 System, the authors identified an off-ladder allele at the Penta D locus as the microvariant allele 9.2. DNA from individuals with the 9.2 allele was amplified using a single primer pair specific for Penta D, and the amplification products were purified and sequenced to characterize the microvariant allele. PCR products were purified using the Wizard® PCR Preps DNA Purification System. Sequence analysis revealed that the 9.2 allele has 10 STR repeats and a TAA deletion in the 3´ flanking region.
(0003771) |
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Products: PowerPlex® 16 System | Wizard® PCR Preps DNA Purification System |
| 9. |
De Maesschalck, K,. Vanhoutte, E., Knaepen, K., Vanderheyden, N., Cassiman, J.J., and Decorte, R.
(2007)
Y-chromosomal STR haplotypes in a Belgian population sample and identification of a micro-variant with a flanking site mutation at DYS19.
Forensic Sci. Int.
152
,
89–94
.
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Notes:
The authors collected DNA samples from 113 unrelated Belgian males. The PowerPlex® Y System and a GeneAmp® 9700 PCR system were used to amplify 12 Y-chromosome STR loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Amplification products were detected using an ABI PRISM® 3100 genetic analyzer and POP-6™ polymer. Allele and haplotype frequencies and haplotype diversity were calculated. A total of 99 different haplotypes were observed.
(0003655) |
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Products: PowerPlex® Y System |
| 10. |
Zhang, H., Li, Y., Jiang, J., Zhang, J., Wu, J., Du, H., Yan, J., Shen, Y. and Hou, Y.
(2007)
Analysis of 15 STR loci in Chinese population from Sichuan in West China.
Forensic Sci. Int.
171
,
222–225
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Notes:
The authors generated population data from 200 unrelated individuals in the Sichuan area of China using the PowerPlex® 16 System. DNA was isolated from blood by phenol:chloroform extraction and quantitated by UV spectrometry. One nanogram of DNA was amplified, and the amplification products were analyzed on an ABI PRISM® 310 Genetic Analyzer.
(0003810) |
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Products: PowerPlex® 16 System |
| 11. |
Davoren, J., Vanek, D., Konjhodzic, R., Crews, J., Huffine, E. and Parsons, T.J.
(2007)
Highly effective DNA extraction method for nuclear short tandem repeat testing of skeletal remains from mass graves.
Forensic Sci. Int.
48
,
478–85
.
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Notes:
The authors compared two DNA extraction methods: the International Commission on Missing Persons silica method and the standard phenol:chloroform method to determine the preferred method for extraction of DNA from skeletal remains. The efficacy of DNA extraction was measured by real-time PCR to quantify DNA and to check for the presence of PCR inhibitors, and by amplification with the PowerPlex® 16 System. DNA was extracted from processed bone powder, and 10µl of the final extract was amplified using the PowerPlex® 16 System and GeneAmp® PCR System 9700 according to the manufacturer's recommendations, except that the extension time was doubled from 30 seconds to 60 seconds for the first 10 cycles and from 45 seconds to 90 seconds for the next 22 cycles. Amplified products were detected using the ABI PRISM® 3100 Genetic Analyzer. The authors concluded that the silica-based method gave better results in autosomal STR typing than the organic extraction method.
(0003818) |
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Products: PowerPlex® 16 System |
| 12. |
Marjanovic, D., Durmic-Pasic, A., Bakal, N., Haveric, S., Kalamujic, B., Kovacevic, L., Ramic, J., Pojskic, N., Skaro, V., Projic, P., Bajrovic, K., Hadziselimovic, R., Drobnic, K., Huffine, E., Davoren, J. and Primorac, D.
(2007)
DNA identification of skeletal remains from the World War II mass graves uncovered in Slovenia.
Croat. Med. J.
48
,
513–519
.
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Notes:
The authors used the PowerPlex® 16 System to perform DNA typing of 27 sets of World War II skeletal remains found in two mass graves in Slovenia. Each bone sample was sanded to remove potential contaminants from exterior surfaces, then washed and air-dried. The same procedure was used to process teeth, except that the teeth were not sanded. DNA was isolated from the bone powder using organic extraction, then quantified. DNA was amplified using the PowerPlex® 16 System as per the manufacturer's recommendations; for samples with small amounts of DNA, the number of cycles was increased to 32 and the elongation time extended to 90 seconds. Fifteen sets of remains yielded full profiles, and 12 sets yielded partial profiles, with the least successful profile including 13 loci. DNA was also extracted and amplified from 69 reference buccal swab samples from potential relatives.
(0003817) |
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Products: PowerPlex® 16 System |
| 13. |
Narkuti, V., Vellanki, R.N., Gandhi, K.P., Doddapaneni, K.K., Yelavarthi, P.D. and Mangamoori, L.N.
(2007)
Microsatellite mutation in the maternally/paternally transmitted D18S51 locus: two cases of allele mismatch in the child.
Clinica Chimica Acta
381
,
171-175
.
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Notes:
The authors describe two cases of paternity dispute, one with a maternally mismatched allele at the D18S51 locus and a second with a paternally mismatched D18S51 allele. Seventeen autosomal STR loci were analyzed using the PowerPlex® 16 System and AmpFlSTR® Identifiler® kit. Amplifications were performed using a GeneAmp® PCR System 9700, and amplification products were detected using an ABI PRISM® 310 Genetic Analyzer. Y-STR loci and mitochondrial DNA hypervariable regions HV1 and HV2 were also examined. Sequence analysis of the D18S51 locus revealed an expansion of the maternal allele by one repeat unit in one case and an expansion of the paternal allele by two repeat units in the second case.
(0003809) |
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Products: PowerPlex® 16 System |
| 14. |
Hill, C.R., Kline, M.C., Mulero, J.J., Lagacé, R.E., Chang, C.W., Hennessy, L.K. and Butler, J.M.
(2007)
Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits.
J. Forensic Sci.
52
,
870–3
.
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Notes:
The authors analyzed 1,308 samples for concordance between the Identifiler® kit, AmpFlSTR® Minifiler™ kit and PowerPlex® 16 System. DNA was isolated from liquid blood using the manual DNA IQ™ System protocol, and STR amplifications were performed as per the manufacturer's recommendations except that reaction volumes were decreased by half. Amplified products were analyzed using an Applied Biosystems 3130xl and POP™-4 or POP™-6 polymer. Twenty seven disconcordant phenotypes were identified between the Minifiler™ and Identifiler® kits, 14 between Minifiler™ and PowerPlex® 16 kits, and 4 between PowerPlex® 16 and Identifiler® kits.
(0003770) |
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Products: DNA IQ™ System | PowerPlex® 16 System |
| 15. |
Vanderheyden, N., Mai, A., Gilissen, A., Cassiman, J.J. and Decorte, R.
(2007)
Identification and sequence analysis of discordant phenotypes between AmpFlSTR SGM Plus™ and PowerPlex® 16.
Int. J. Legal Med.
121
,
297–301
.
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Notes:
The authors analyzed duplicate buccal swabs and observed eight disconcordant phenotypes with SGM Plus™ (2 at TH01, 4 at vWA and 2 at D18S51) and one disconcordant phenotype (at D18S51) with PowerPlex® 16 out of a total of 1,377 individuals. In each case, the discrepancy was due to allele dropout of the second allele in a heterozygous genotype. DNA sequencing using primers that encompass the locus-specific STR primers was performed to characterize the mutations responsible for allele dropout for each locus.
(0003769) |
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Products: PowerPlex® 16 System |
| 16. |
Bain, J.M., Tavanti, A., Davidson, A.D., Jacobsen, M.D., Shaw, D., Gow, N.A. and Odds, F.C.
(2007)
Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus.
J. Clin. Microbiol.
45
,
1469–1477
.
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Notes:
The authors developed a multilocus sequence typing scheme (MLST) to examine sequence variation and discriminate between Aspergillus fumigatus strains. They also examined the distribution of MAT1-1 and MAT1-2 sexual idiomorphs in 100 clinical and environmental isolates. Sexual idiomorphs were determined using PCR and a reverse primer to both idiomorphs and a forward primer specific to either MAT-1 or MAT-2. PCRs consisted of 2mM MgCl2, 200µM DNTPs and 2.5 units of GoTaq® DNA Polymerase.
(0003714) |
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Products: GoTaq® DNA Polymerase |
| 17. |
Kraaijenbrink, T., van Driem, G.L., Opgenort, J.R., Tuladhar, N.M. and de Knijff, P.
(2007)
Allele frequency distribution for 21 autosomal STR loci in Nepal.
Forensic Sci. Int.
168
,
227–231
.
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Notes:
Allele frequency data for 21 autosomal loci was studied in 953 unrelated individuals belonging to 12 major Nepalese groups. DNA was extracted from whole blood using the Autopure LS® kit, then amplified using the PowerPlex® 16 System, AmpFlSTR® Identifiler® kit or F13A01, FESFPS, F13B, LPL Multiplex (FFFL) Multiplex. Amplification products were analyzed using the ABI PRISM® 3100 Genetic Analyzer. Several new alleles not reported in the NIST short tandem repeat database were detected in this population.
(0003811) |
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Products: F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein) | PowerPlex® 16 System |
| 18. |
Kraaijenbrink, T., van Driem, G.L., Tshering of Gaselô, K. and de Knijff, P.
(2007)
Allele frequency distribution for 21 autosomal STR loci in Bhutan.
Forensic Sci. Int.
170
,
68–72
.
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Notes:
The authors determined the allele frequencies for 21 autosomal STR loci in 936 individuals from the Royal Kingdom of Bhutan using the AmpFlSTR® Identifiler® kit, PowerPlex® 16 System and the F13A01, FESFPS, F13B, LPL (FFFL) Multiplex. DNA was extracted from whole blood samples using the Autopure LS® kit and amplified as directed by the manufacturers. Amplified products were detected using an ABI PRISM® 3100 Genetic Analyzer.
(0003822) |
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Products: F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein) | PowerPlex® 16 System |
| 19. |
Rapone, C., Geraci, A., Capelli, C., De Meo, A., D'Errico, G., Barni, F., Berti, A. and Lago, G.
(2007)
Y chromosome haplotypes in Central-South Italy: Implication for reference database.
Forensic Sci. Int.
172
,
67–71
.
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Notes:
Y-chromosome haplotypes of 150 males from 10 regions in Italy were determined using the PowerPlex® Y System and the ABI PRISM®3100 genetic analyzer. DNA samples were isolated from blood using the DNA IQ™ System. The total number of haplotypes oberved was 141, and 146 of the haplotypes were unique. Ten samples showed locus duplication.
(0003686) |
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Products: DNA IQ™ System | PowerPlex® Y System |
| 20. |
Fernandes, A.T., Fernandes, S., Gonçalves, R., Sá, R., Costa, P., Rosa, A., Ferrás, C., Sousa, M., Brehm, A., and Barros, A.
(2006)
DAZ gene copies: evidence of Y chromosome evolution.
Mol. Human Reprod.
12
,
519–23
.
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Notes:
The polymorphic DAZ gene family is located at the AZFc region of the human Y chromosome, and mutations or deletions within these genes can contribute to male infertility. The authors found an association between DAZ haplotypes and Y-STR haplotypes and used variation within 10 Y-STR loci to determine the coalescence time of DAZ haplotypes. The Y-STR haplotypes were determined using the PowerPlex® Y System.
(0003658) |
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Products: PowerPlex® Y System |
| 21. |
Morikawa, T., Nakaki, S., Moriyoshi, H., Nakayama, H., Hino, D., Miyoshi, M. and Itohara, K.
(2006)
Allele frequencies and haplotypes of the 12 Y-STR loci using the PowerPlex Y system in Japanese population.
J. Forensic Sci.
51
,
941–944
.
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Notes:
The authors isolated DNA from 153 unrelated individuals fron the Hiroshima prefecture in Japan. Amplifications were carried out with 0.2–0.5ng of DNA and 10/20 cycling, and amplification products were detected using an ABI PRISM® 3100 genetic analyzer. Allele frequencies and gene diversity values are presented.
(0003644) |
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Products: PowerPlex® Y System |
| 22. |
Turrina, S., Atzei, R. and De Leo, D.
(2006)
Haplotype analysis of the PowerPlex® Y System in Northeast population from Italy.
Int. Congr. Ser.
1288
,
265–7
.
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Notes:
The authors generated population data and Y-STR haplotype frequencies for 105 unrelated males and 50 father-son pairs in northeast Italy. DNA was collected as blood or saliva samples, isolated then amplified using the PowerPlex® Y System as directed by the manufacturer. Amplified products were detected using an ABI PRISM® 3100-Avant Genetic Analyzer.
(0003867) |
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Products: PowerPlex® Y System |
| 23. |
Pepinski, W., Niemcunowicz-Janica, A., Skawronska, M., Janica, J.R., Koc-Zorawska, E., Janica, J. and Soltyszewski, I.
(2006)
Y-chromosome variation in northeastern Poland.
Int. Congr. Ser.
1288
,
249–51
.
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Notes:
The authors generated Y-STR haplotype frequency data for several ethnic populations in Poland using the PowerPlex® Y System. DNA was isolated using Chelex® resin and a proteinase K protocol and amplified, and amplification products were detected using an ABI PRISM® 310 Genetic Analyzer.
(0003869) |
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Products: PowerPlex® Y System |
| 24. |
Haas, C., Wangensteen, T., Giezendanner, N., Kratzer, A. and Bär, W.
(2006)
Y-chromosome STR haplotypes in a population sample from Switzerland (Zurich area).
Forensic Sci. Int.
158
,
213–218
.
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Notes:
The authors collected buccal swabs from 150 unrelated Swiss males and isolated DNA for use in determination of haplotype frequencies of 12 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Purified DNA was amplified using the PowerPlex® Y System and a total of 30 cycles, and amplification products were detected using an ABI PRISM® genetic analyzer. Allele frequencies were calculated by direct counting.
(0003647) |
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Products: PowerPlex® Y System |
| 25. |
Opel, K.L., Chung, D.T., Drábek, J., Tatarek, N.E., Jantz, L.M. and McCord, B.R.
(2006)
The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains.
J. Forensic Sci.
51
,
351–356
.
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Notes:
The authors developed a new set of miniplex primers for DNA typing of degraded DNA from human skeletal remains. The miniplex primers produced smaller amplicons (50–280 base pairs) than standard STR systems. The DNA-typing results obtained with the miniplex primers were compared to results obtained with the PowerPlex® 16 System. The authors determined that larger loci failed to amplify when using degraded DNA and that the degradation cut-off length of template fragments occurred predominantly at 200bp and is not kit-dependent.
(0003808) |
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Products: PowerPlex® 16 System |