|
| 1. |
Wu, Y., Connors, D., Barber, L., Jayachandra, S., Hanumegowda, U.M. and Adams, S.P.
(2009)
Multiplexed assay panel of cytotoxicity in HK-2 cells for detection of renal proximal tubule injury potential of compounds
Toxicology in Vitro
23
,
1170-1171
.
|
| |
Notes:
The authors describe a multiplexed in vitro assay to detect nephrotoxicity and gain information about mechanism of cell death in HK-2 (human kidney-2) cells. The multiplexed assay involved an LDH assay to detect necrosis, a caspase-3/7 assay to detect apoptosis, a reazurin assay to assess metabolic state, and a DNA dye staining assay to monitor nuclear morphology.
(0004002) |
| |
 |
| |
Products: Caspase-Glo® 3/7 Assay | Caspase-Glo® 3/7 Buffer | Caspase-Glo® 3/7 Substrate | CellTiter-Blue® Cell Viability Assay | CellTiter-Glo® Buffer | CellTiter-Glo® Luminescent Cell Viability Assay | CellTiter-Glo® Substrate (lyophilized) | CytoTox-ONE™ Homogeneous Membrane Integrity Assay | CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP |
| 2. |
Niles, A.L., Moravec, R.A. and Riss, T.L.
(2009)
In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high-throughput screening
Current Chemical Genomics
3
,
33-41
.
|
| |
Notes:
The authors review the use of in vitro cytotoxicity testing in drug discovery to characterize the toxic potential of new chemical entities (nce) at the earliest stages of profiling. DOI: 10.2174/1875397300903010033
(0004000) |
| |
|
| |
Products: Apo-ONE® Homogeneous Caspase-3/7 Assay | Apo-ONE® Homogeneous Caspase-3/7 Buffer | Caspase-Glo® 3/7 Assay | CellTiter-Blue® Cell Viability Assay | CellTiter-Fluor™ Cell Viability Assay | CellTiter-Glo® Assay Custom Solution | CellTiter-Glo® Luminescent Cell Viability Assay | MultiTox-Fluor Multiplex Cytotoxicity Assay | MultiTox-Glo Multiplex Cytotoxicity Assay |
| 3. |
Gupta, P.B., Onder, T.T., Jiang, G., Tao, K., Kuperwasser, C., Weinberg, R.A. and Lander, E.S.
(2009)
Identification of selective inhibitors of cancer stem by high-throughput screening.
Cell
138
,
645-59
.
|
| |
Notes:
The authors of this study describe a proof-of-concept screen to use mammary epithelial cells that have been induced to undergo an epithelial to mesenchymal transition (EMT) as model cells to identify agents that may be selectively toxic against "epithelial cancer stem cells" (CSCs). They induced the transformed breast cancer cell line HMLER to undergo a mesenchymal transition using shRNA directed against the E-cadherin gene. They characterized the responsiveness of these transitioned cells to common cytotoxic agents using the CellTiter® 96 AQueous Cytoxicity Assay and compared the response to that of HMLER cells containing a control shRNA. They showed that the HMLER cells induced to undergo EMT behaved more like CSCs. The researchers then performed a proof-of-concept high-throughput screen to identify compounds that targeted the HMLER cells induced to undergo EMT, using the CellTiter-Glo® Assay to assess cell viability.
(0004006) |
| |
|
| |
Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | CellTiter-Glo® Assay Custom Solution | CellTiter-Glo® Buffer | CellTiter-Glo® Luminescent Cell Viability Assay | CellTiter-Glo® Substrate (lyophilized) |
| 4. |
Grenier, A.L., Abu-Ihweij, K., Zhang, G., Ruppert, S.M., Boohaker, R., Slepkov, E.R., Pridemore, K., Ren, J.J., Fliegel, L. and Khaled, A.R.
(2008)
Apoptosis-induced alkalinization by the Na+/H+ exchanger isoform 1 is mediated through phosphorylation of amino
Am. J. Physiol. Cell Physiol.
295
,
C883-C896
.
|
| |
Notes:
The authors wanted to examine the role of plasma membrane protein Na+/H+ exchanger isoform 1 (NHE1) in apoptosis. API cells, a NHE1-deficient Chinese hamster ovary cell line, was cotransfected with wild-type NHE1 or mutant NHE1 constructs and destabilized yellow fluorescent protein (YFP). Cells were plated at a density of 1 × 104 cells/well in a 96-well plate with or without FBS. To induce apoptosis in the cells, serum was withdrawn for 24 hours. The ratio of dead-to-live cells was measured using the MultiTox-Fluor Multiplex Cytotoxicity Assay. Cell death was also determined by examining the loss of YFP fluorescence under a microscope.
(0003937) |
| |
|
| |
Products: MultiTox-Fluor Multiplex Cytotoxicity Assay |
| 5. |
Hahn, C.K., Ross, K.N., Warrington, I.M., Mazitschek, R., Kanegai, C.M., Wright, R.D., Kung, A.L., Golub, T.R. and Stegmaier, K.
(2008)
Expression-based screening identifies the combination of histone deacetylase inhibitors and retinoids for neuroblastoma differentiation.
Proc. Natl. Acad. Sci. USA
105
,
9751-9756
.
|
| |
Notes:
The authors designed a high-throughput gene-expression screen to identify compounds that induce a neuroblastoma gene signature in BE(2)-C cells. The screen used Valproic Acid (VPA), an inhibitor of histone deacetylase, as an enhancer. The top hit from the screen was all-trans retinoic acid (ATRA). The authors proposed that ATRA + VPA would reduce cell viability and might induce apoptosis by inducing genes associated with terminal differentiation. They used the CellTiter-Glo® Luminescent Cell Viability Assay in a 96-well format to assess the effects of ATRA and a variety of inhibitors of histone deacetylase on BE(2)-C cell viability.
(0003931) |
| |
|
| |
Products: CellTiter-Glo® Luminescent Cell Viability Assay |
| 6. |
Case, M., Matheson, E., Minto, L., Hassan, R., Harrison, C.J., Bown, N., Bailey, S., Vormoor, J., Hall, A.G. and Irving, J.A.E.
(2008)
Mutation of genes affecting the RAS pathway is common in childhood acute lymphoblastic leukemia
Cancer Res.
68
,
6803-6809
.
|
| |
Notes:
The authors of this study investigated the relationship of somatic mutations that deregulate the RAS-RAF-MEK-ERK pathway and Acute Lymphoblastic Leukemia (ALL) and its progression to relapse in some patients. They show that such mutations are common in ALL and its recurrence. Furthermore, lymphoblasts from patients with mutations that were associated with upregulation of ERK showed increased cytotoxicity over wild-type controls when treated with U0126, suggesting that specific ERK inhibitors may eventually yield useful therapeutics. Following drug exposure, cytotoxic effects were assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay.
(0003905) |
| |
|
| |
Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay | MEK Inhibitor U0126 |
| 7. |
Yoon, J.-J., Chawla, D., Pall, T., Ndungu, M., Du, Y., Kurtkaya, S., Sun, A., Snyder, J.P. and Plemper, R.K.
(2008)
High-throughput screening-based identification of paramyxovirus inhibitors.
J. Biomol. Screen
13
,
591-608
.
|
| |
Notes:
The authors describe an HTS assay to screen for inhibitors of measles virus infection. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess toxicity of all confirmed hit compounds from the primary screen. A luminescent assay was used to assess cell-to-cell fusion in the presence candidate compounds that appeared to inhibit entry. Effector cells that expressed the T7 polymerase and measles virus H and F envelope proteins were overlaid on target cells expressing firefly luciferase under the control of a T7 polymerase promoter. Inhibition of fusion should reduce luciferase expression compared to a positive fusion control. Ten of the eleven compounds tested caused a dose-dependent reduction in luciferase expression, suggesting they block viral entry into cells. Luminescence was detected using the Bright-Glo™ Assay System.
(0003933) |
| |
|
| |
Products: Bright-Glo™ Luciferase Assay System | CytoTox 96® Non-Radioactive Cytotoxicity Assay |
| 8. |
Prichard, M.N., Daily, S.L., Jefferson, G.M., Perry, A.L. and Kern, E.R.
(2007)
A rapid DNA hybridization assay for the evaluation of antiviral compounds against Epstein-Barr virus.
J. Virol. Methods.
144
,
86–90
.
|
| |
Notes:
The authors developed an assay to evaluate antiviral compounds for Epstein-Barr virus (EBV) infections. EBV DNA was isolated using the Wizard® SV 96 Genomic DNA Purification System, and 5µl was used for real-time PCR to quantify the viral DNA. Cytotoxicity of the antiviral compounds was assessed using treated, uninfected Akata cells compared to those with no treatment or infection. After three days when the viral DNA was harvested, cell viability was measured using the CellTiter-Glo® Luminescent Cell Viability Assay.
(0003761) |
| |
|
| |
Products: CellTiter-Glo® Luminescent Cell Viability Assay | Wizard® SV 96 Genomic DNA Purification System |
| 9. |
Wu, D., Ren, Z., Pae, M., Guo, W., Cui, X., Merrill, A.H. and Meydani, S.N.
(2007)
Aging up-regulates expression of inflammatory mediators in mouse adipose tissue.
J. Immunol.
179
,
4829–4839
.
|
| |
Notes:
The authors examined the role of ceramide and NF-κB in age-related adipose tissue inflammation in mice. Levels of inflammation-associated molecules, IL-1β, IL-6, TNF-α, COX-2 and peroxisome proliferator-activated receptor (PPAR)-γ mRNA, were quantified by quantitive PCR (qPCR). RNA was isolated from adipose tissues, and first-strand cDNA was synthesized using the Reverse Transcription System prior to qPCR. mRNA levels were also quantified in peritoneal macrophages grown in the presence of young adipocyte-conditioned medium and old adipocyte-conditioned medium. Viability of the primary adipocytes used in these experiments was confirmed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay and CytoTox 96® Non-Radioactive Cytotoxicity Assay.
(0003777) |
| |
|
| |
Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay | CytoTox 96® Non-Radioactive Cytotoxicity Assay | Reverse Transcription System |
| 10. |
Niles, A.L., Moravec, R.A., Hesselberth, P.E., Scurria, M.A., Daily, W.J. and Riss, T.L.
(2007)
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Anal. Biochem.
366
,
197–206
.
|
| |
Notes:
The authors of this paper describe an assay that uses protease biomarkers to assess cell viability and cell death simultaneously in a population of cells. The assay detects an ubiquitous protease activity that is associated with live cells and a second protease activity that is associated with cells that have lost membrane integrity. The readouts are either fluorescent or fluorescent and luminescent. The assay can be performed in multiplex with other assays, such as caspase assays, to gain additional information on the cell population, and it is amenable to high-throughput screening.
(0003927) |
| |
|
| |
Products: CytoTox-Glo™ Cytotoxicity Assay | MultiTox-Fluor Multiplex Cytotoxicity Assay | MultiTox-Glo Multiplex Cytotoxicity Assay |
| 11. |
Rossi, C., Padmanaban, D., Ni, J., Yeh, L-A., Glicksman, M.A. and Waldner, H.
(2007)
Identifying druglike inhibitors of myelin-reactive T cells by phenotypic high-throughput screening of a small-molecule library.
J. Biomol. Screen.
12
,
481–489
.
|
| |
Notes:
The authors of this study sought to identify inhibitors of myelin-reactive T-cell proliferation. They used spleen cells isolated from transgenic mice expressing T-cell receptors that specifically recognize myelin proteolipid protein residues 139-151 (PLP139-151). Spleen cell suspensions were prepared from the transgenic mice and exposed to PLP in the presence of test compounds. Proliferation was assessed relative to positive and negative controls using the CellTiter-Glo® Luminescent Cell Viability Assay. Of the 41,184 compounds screened, 302 hits were obtained. These 302 compounds were next evaluated for nonspecific cytotoxicity using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay, and compounds causing nonspecific toxicity were eliminated from further consideration. Z´-factor values for the primary screen were robust (> 0.5).
(0003733) |
| |
|
| |
Products: CellTiter-Glo® Luminescent Cell Viability Assay | CytoTox-ONE™ Homogeneous Membrane Integrity Assay | CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP |
| 12. |
Chaea, S.Y., Leeb, M., Kimb, S.W. and Baeb, Y.H.
(2004)
Protection of insulin secreting cells from nitric oxide induced cellular damage by crosslinked hemoglobin.
Biomaterials
25
,
843–850
.
|
| |
Notes:
The authors studied the effect of nitric oxide stress on rat Islets of Langerhans cells and rat insulinoma cell line (RINm5F) and to determine if cross-linked hemoglobin (Hb-C)could mediate the stress and subsequent apoptosis. The rat cells were treated with a nitric oxide donor SNAP and the DeadEnd™ Colorimetric TUNEL System was used to measure the effect of NO with or without Hb-C. In addition, the cell viability was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. Twenty-four hours after SNAP treatment, the level of NO2 (a by-product of NO) was measured using Griess Reagent to determine the level of nitric oxide production induced.
(0003053) |
| |
|
| |
Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay | DeadEnd™ Colorimetric TUNEL System | Griess Reagent System |
| 13. |
Spagnou, S., Miller, A.D. and Keller, M.
(2004)
Lipidic carriers of siRNA: Differences in the formulation, cellular uptake, and delivery with plasmid DNA.
Biochemistry
43
,
13348-13356
.
|
| |
Notes:
The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to determine the cytotoxic effect of lipophilic transfection reagents commonly used for siRNA transfection. Data are presented as the percent cell death observed in HeLa and IGROV-1 cells at 24 hours post-transfection. The researchers tested the effect of each reagent with and without siRNA during transfections.
(0003178) |
| |
|
| |
Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay |
| 14. |
Lee, J.M., Shih, A.Y., Murphy, T.H., and Johnson, J.A.
(2003)
NF-E2-related factor-2 mediates neuroprotection against mitochondrial complex I inhibitors and increased concentrations of intracellular calcium in primary cortical neurons.
J. Biol. Chem.
278
,
37948–37956
.
|
| |
Notes:
NF-E2-related factor-2 (NRF2) upregulates transcription of antioxidant response element (ARE)-driven genes. The authors examined the sensitivity of Nrf-2–/– primary mouse neurons to oxidative stress-induced apoptosis using the mitochondrial toxins 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine (MPP+) and rotenone. Primary cultures were >090% neurons, as judged by immunostaining with the Anti-βIII Tubulin mAb and other neuron-specific antibodies. Neuronal cytotoxicity in the presence of MPP+ and rotenone was monitored using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay and TUNEL assays. The level of expression of the antioxidant-responsive gene quinone oxidoreductase was measured by mRNA quantitation using RT-PCR, enzyme activity assays and immunocytochemistry. The Reverse Transcription System was used to synthesize cDNA for subsequent amplification by PCR.
(0003570) |
| |
|
| |
Products: Anti-βIII Tubulin mAb | CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | Reverse Transcription System |
| 15. |
Hernández, J.M., Bui, M.H.T., Han, K-r., Mukouyama, H., Freitas, D.G., Nguyen, D., Caliliw, R., Shintaku, P.I., Paik, S.H., Tso, C-L., Figlin, R.A., Belldegrun, A.S.
(2003)
Novel kidney cancer immunotherapy based on the granulocyte-macrophage colony-stimulating factor and carbonic anhydrase IX fusion gene
Clin. Cancer Res.
9
,
1906-1916
.
|
| |
Notes:
pGEM®-T Easy Vector was used to clone PCR products. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to determine specific cytotoxicity of human dendritic cells that were transduced with recombinant adenoviruses containing the gene encoding a fusion protein of granulocyte-macrophage colony stimulating factor and carbonic anhydrase IX.
(0002674) |
| |
|
| |
Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay | pGEM®-T Easy Vector System I | pGEM®-T Easy Vector System II |
| 16. |
Kato, A., Okaya, T., and Lentsch, A.B.
(2003)
Endogenous IL-13 protects hepatocytes and vascular endothelial cells during ischemia/reperfusion injury.
Hepatology
37(2)
,
304-312
.
|
| |
Notes:
Mouse hepatocytes (AML-12 cell line) were assayed for cytotoxicity to 1 mmol/L hydrogen peroxide with and without 20ng/mL IL-13 using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay. For these assays 1 x 105 cells/well were seeded in 96-well microplates and cultured for 24 hours before IL-13 treatment. One hour later, hydrogen peroxide was added to the cultures. The cells were then cultured for an additional 24 hours before analysis with the CytoTox-ONE™ Homogeneous Membrane Integrity Assay.
(0003030) |
| |
|
| |
Products: CytoTox-ONE™ Homogeneous Membrane Integrity Assay | CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP |
| 17. |
Suzuki, Y., Shibata, K., Kikkawa, F., Kajiyama, H., Kazuhiko, I., Nomura, S., Tsujumoto, M., Mizutani, S.
(2003)
Possible role of placental leucine aminopeptidase in the antiproliferative effect of oxytocin in human endometrial adenocarinoma
Clin. Cancer Res.
9
,
1528-1534
.
|
| |
Notes:
The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to evaluate the effect of oxytocin on proliferation of human endometrial endometriod adenocarcinoma cell lines.
(0002654) |
| |
|
| |
Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 18. |
Gaunitz, F. and Heise, K.
(2003)
HTS Compatible Assay for Antioxidative Agents Using Primary Cultured Hepatocytes
Assay Drug Dev. Technol.
3
,
469-477
.
|
| |
Notes:
Primary hepatocytes from Sprauge-Dawley rats were incubated with various concentrations of tert-butylhydroperoxide to induce oxidative stress and then cell viability, cytotoxicity and apoptosis were assayed with Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay, CellTiter-Blue Cell Viability Assay, CellTiter-Glo Luminescent Cell Viability Assay, CytoTox-ONE Homogeneous Membrane Integrity Assay, Apo-ONE Homogeneous Caspase-3/7 Assay and assay systems.
(0002969) |
| |
|
| |
Products: Apo-ONE® Homogeneous Caspase-3/7 Assay | Apo-ONE® Homogeneous Caspase-3/7 Buffer | CellTiter 96® AQueous One Solution Cell Proliferation Assay | CellTiter-Blue® Cell Viability Assay | CellTiter-Glo® Luminescent Cell Viability Assay | CytoTox-ONE™ Homogeneous Membrane Integrity Assay | CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP |
| 19. |
Theodossiou, T., Hothersall, J.S., Woods, E.A., Okkenhaug, K., Jacobson, J. and MacRobert, A.J.
(2003)
Firefly luciferin-activated Rose Bengal: In vitro photodynamic therapy by
intracellular chemiluminescence in transgenic NIH 3T3 cells.
Cancer Res.
63
,
1818-1821
.
|
| |
Notes:
NIH 3T3 cells were transfected using Superfect (Qiagen) with Promega's pGL3-Control Vector and a marker for antibiotic resistance at a 5:1 ratio. Transfected cells were visualized after in situ addition of 500μM Beetle Luciferin in 10% FCS medium. The transfected cells were used to show that light from a luciferase reaction can be used to create targeted cytotoxicity in the presence of the photosensitizer Rose Bengal (RB). This application has specific relevance as a tumor treatment by photodynamic therapy.
(0002671) |
| |
|
| |
Products: Beetle Luciferin, Potassium Salt | pGL3-Control Vector |
| 20. |
Mellström, B., Ceña, V., Lama, M., Perales, C., Gonzalez, C., Naranjo, J.R.
(2002)
Gas1 is induced during and participates in excitotoxic neuronal death
Mol. Cell. Neurosci.
19
,
417-29
.
|
| |
Notes:
Transiently transfected primary neuronal cells expressing beta-galactosidase were treated with NMDA and stained with X-Gal using the Promega method. Cytotoxicity of the treated cells was analyzed using the CytoTox 96® Non-Radioactive Cytotoxicity Assay.
(0002580) |
| |
|
| |
Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay | X-Gal |
| 21. |
Hardingham, G.E., Fukunaga, Y., Bading, H.
(2002)
Extrasynaptic NMDARs oppose synaptic NMDARs by triggering CREB shut-off and cell death pathways
Nat. Neurosci.
5
,
405-414
.
|
| |
Notes:
Hipppocampal neurons were cultured for 10-12 days and then hypoxic or ischemic conditions were induced. Eight hours after this event acute cell death was quantified by measurements of LDH release using Promega's CytoTox 96® Non-Radioactive Cytotoxicity Assay.
(0002439) |
| |
|
| |
Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay |
| 22. |
Mizumoto, N., Kumamoto, T., Robson, S.C., Sevigny, J., Matsue, H., Enjyoji, K., Takashima, A.
(2002)
CD39 is the dominant Langerhans cell-associated ecto-NTPDase: Modulatory roles in inflammation and immune responsiveness
Nat. Med.
8
,
358-365
.
|
| |
Notes:
Pam-212 keratinocytes (murine) or freshly isolated epidermal cells were treated in PBS with a variety of skin irritants in an attempt to stimulate release of ATP from the cells. Supernatants from the cell cultures were examined for ATP concentrations using a luciferin-luciferase assay and for LDH release using Promega's CytoTox 96® Assay. The percent -specific release of LDH was calculated based on total LDH concentration detected in the supernatants after permeabilization with 0.3% Triton X-100. The authors conclude that injured keratinocytes release ATP.
(0002431) |
| |
|
| |
Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay |
| 23. |
Tonello, F., Seweso, M., Marin, O., Mock, M., and Montecucco, C.
(2002)
Screening inhibitors of anthrax lethal factor.
Nature
418
,
386
.
|
| |
Notes:
This brief communication discusses substrates of anthrax lethal factor that can be used for high-throughput screening of potential inhibitors. The CellTiter® Aqueous Cell Proliferation Assay was used to assess the effect of selected inhibitors on cytotoxicity of lethal factor in RAW264.7 cells.
(0002555) |
| |
|
| |
Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 24. |
Rees, D.A., Lewis, M.D., Lewis, B.M., Smith, P.J., Scanlon, M.F., and Ham, J.
(2002)
Adenosine-regulated cell proliferation in pituitary folliculostellate and endocrine cells: differential roles for the A1 and A2B adenosine receptors.
Endocrinology
143
,
2427-2436
.
|
| |
Notes:
Promega's CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to examine the effects of adenosine and adenosine receptor agonists on a pituitary folliculostellate cell line and two pituitary endocrine cell lines. The authors also used RQ1 RNase-free DNase during the course of their experiments.
(0002495) |
| |
|
| |
Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | RQ1 RNase-Free DNase |
| 25. |
Krebs, F.C., Miller, S.R., Catalone, B.J., Fichorova, R., Anderson, D., Malamud, D., Howett, M.K., and Wigdahl, B.
(2002)
Comparative in vitro sensitivities of human immune cell lines, vaginal and cervical epithelial cell lines, and primary cells to candidate microbicides nonoxynol 9, C31G, and sodium dodecyl sulfate.
Antimicrob. Agents Chemother.
46(7)
,
2292-2298
.
|
| |
Notes:
In this paper, cytotoxicity results obtained using an MTT-based assay were confirmed using the CellTiter-96® AQueous Non-Radioactive Cell Proliferation Assay and the CellTiter-Glo™ Luminescent Cell Viability Assay on primary human vaginal keratinocyte cultures and immortalized VK2/E6E7 cells.
(0002605) |
| |
|
| |
Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | CellTiter-Glo® Luminescent Cell Viability Assay |