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| 1. |
Hawes, J.J., Nerva, J.D. and Reily, K.M.
(2008)
Novel dual-reporter preclinical screen for antiastrocytoma agents identifies cytostatic and cytotoxic compounds
J. Biolmol. Scr.
13
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795-803
.
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Notes:
The authors of this paper created a mouse model for in vitro assays to screen for therapeutic compounds specifically active against astrocytic gliomas. A Green/Red luciferase (G/R-luc) dual-reporter system was created in KR158 cells (derived from grade III agressive mouse anaplastic astrocytoma). The green click beetle luciferase gene (from pCBC68-Basic Vector) was placed under the control of the E2F1 promoter, and the red click beetle luciferase gene (from pCBR-Basic Vector) was placed under the control of the CMV promoter. The dual-reporter assay simultaneously evaluates E2F1 promoter activity and assesses cytotocity; the assay also distinguishes cytostatic from cytotoxic compounds.
(0003949) |
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Products: Chroma-Glo™ Luciferase Assay System | pCBG68-Basic Vector | pCBR-Basic Vector |
| 2. |
Davis, R. E., Zhang, Y-Q., Suthall, N., Staudt, L.M., Austin, C.P., Inglese, J. and Auld, D.S.
(2007)
A cell-based assay for IκBα stabilization using a two-color dual luciferase-based sensor
ASSAY and Drug Development Technologies
5
,
85-103
.
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Notes:
The Chroma-Luc™ vectors, which encode red light-emitting and green-light emitting beetle luciferases, were used to develop an HTS cell-sensor assay (1536-well format) to detect IκBα stabilization. The assay was able to identify specific stabilizers of IκBα. Known and novel inhibitors of NFκB signaling were detected in the assay.
(0003727) |
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Products: Chroma-Glo™ Luciferase Assay System | pCBG68-Basic Vector | pCBG68-Control Vector | pCBG99-Basic Vector | pCBG99-Control Vector | pCBR-Basic Vector | pCBR-Control Vector |
| 3. |
Ogura, R., Matsuo, N., Wako, N., Tanaka, T., Ono, S. and Hiratsuka, K.
(2005)
Multi-color luciferases as reporters for monitoring transient gene expression in higher plants
Plant Biotechnol.
22
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151–5
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Notes:
The authors evaluated use of a dual-color reporter assay for plant gene expression using the red and green light-emitting click beetle luciferase genes from the Chroma-Luc™ vectors (pCBR-Basic Vector, pCBG99-Basic Vector and pCBG68-Basic Vector). Plant expression vectors were constructed based on the reporter plasmid, pBI221, which contains the CaMV35S promoter, GUS reporter and nos-terminator cassette. The GUS cassette was replaced by the CBRluc, CBF99luc and CBR68luc genes. Twenty-five microliters of the gold microcarrier (1.6 mm) coated with 2µg plasmid DNA and were used to bombard plant specimens. Six hours after bombardment, an aqueous solution of 0.1mM D-luciferin potassium salt was sprayed on the transiently-tranfected plants and luminescence detected using a CCD camera system. For the color-specific detection, the researchers used interference filters for wavelengths greater than 610nm (high-pass filter) and wavelengths between 510nm and 560nm (band-pass filter). To test luciferase activity in cell extracts, cultured tobacco (BY-2) and onion epidermal cells were cotranfected by bombardment using the click beetle constructs and a plasmid with the 35S promoter driving Renilla luciferase. The cells were homogenized in Passive Lysis Buffer and 8µl of the prepared lysate was assayed using the Dual-Luciferase® Reporter Assay. A second plasmid containing the inducible CAB1 promoter from Arabidopsis thaliana, was made by replacing the firefly luciferase gene with the CBG99luc-coding sequence from the pCBG99-Basic Vector. Spinach leaves were transfected by bombardment with the CAB1 construct, exposed to different light conditions for 21 hours and luminescence levels detected by CCD camera and interference filters. In all cases, the authors were able to detect click beetle luciferase expression in plants and cultured cells.
(0003293) |
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Products: Dual-Luciferase® Reporter Assay System | Dual-Luciferase® Reporter Assay System 10-Pack | pCBG68-Basic Vector | pCBG99-Basic Vector | pCBR-Basic Vector |
| 4. |
Doan, L.L., Porter, S., Duan, Z., Flubacher, M.M., Montoya, D., Tsichlis, P.N., Horwitz, M., Gilks, C.B. and Grimes, H.L.
(2004)
Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells.
Nucleic Acids Res.
32
,
2508–19
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Notes:
The researchers studied the activity of Growth factor independence-1 (GFI1) transcriptional repressors in T cells. The rat Gfi1 promoter and intron were cloned into the pCBG99-Basic Vector and the GFI1 binding sites subjected to site-directed mutagenesis. Jurkat T cells were then cotransfected with wildtype or mutant Gfi1 promoter constructs and a GFI1 expression vector. Click beetle luciferase activity was measured using the Chroma-Glo™ Luciferase Assay System and compared to that of a control luciferase vector.
(0003284) |
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Products: Chroma-Glo™ Luciferase Assay System | pCBG99-Basic Vector | pCBG99-Control Vector |