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| 1. |
Brem, R., Li, F. and Karran, P.
(2009)
Reactive oxygen species generated by thiopurine/UVA cause irreparable transcription-blocking DNA lesions.
Nucleic Acids Res.
37
,
1951–1961
.
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Notes:
The association of thiopurines (anticancer drugs) with acute skin sensitivity to ultraviolet A (UVA) radiation and a high risk of skin cancer was tested using six human fibroblast and lymphoid cell lines. The thiopurine 6-thioguanine (6-TG) was added at 0.8 or 0.6mM to each of six cell lines and incubated for 48 hours to ensure incorporation. DNA and RNA were extracted and 40µg of nucleic acid were digested to nucleosides, separated by HPLC, and the 6-TG 20-deoxy and ribonucleosides quantified by absorbance at 342 nm. The same DNA isolation and digestion method was used when the cells were treated with 1µM of 6-TG and then irradiated with 5 kJ/m2 UVA after 48 hours. The Wizard® Genomic DNA Purification Kit was used for DNA extraction.
(0004013) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 2. |
Nair, N.U. and Zhao, H.
(2009)
Mutagenic inverted repeat assisted genome engineering (MIRAGE).
Nucleic Acids Res.
37
,
e9
.
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Notes:
In this paper, the researchers describe and demonstrate a new method for creating precise genome modifications in Saccharomyces cerevisiae. The mutagenic inverted repeat assisted genome engineering (MIRAGE) was tested in S. cerevisiae W303a by deleting gal7 as well as point and frameshift mutations. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit, amplified and modifications verified by gel analysis or DNA sequencing.
(0004014) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 3. |
Gill, M.B., Wright, D.E., Smith, C.M., May, J.S. and Stevenson, P.G.
(2009)
Murid herpesvirus-4 lacking thymidine kinase reveals route-dependent requirements for host colonization.
J. Gen. Virol.
90
,
1461–1470
.
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Notes:
The authors examined the role of thymidine kinase (TK) in establishing a herpesvirus infection via the upper respiratory tract. DNA was purified from ex vivo organs of female BALB/c mice infected with a murid herpesvirus-4 (MuHV-4) TK knockout using the Wizard® Genomic DNA Purification Kit. Real-time PCR was used with 50–80ng of purified DNA to determine viral load of the animals.
(0004015) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 4. |
Acke, E., McGill, K., Golden, O., Jones, B.R., Fanning, S. and Whyte, P.
(2009)
Prevalence of thermophilic Campylobacter species in household cats and dogs in Ireland.
Vet. Rec.
164
,
44–47
.
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Notes:
To examine the prevalence of Campylobacter species in asymtomatic carriers that can pass the bacteria onto humans, rectal swabs were collected from 147 dogs and 35 cats in Ireland and cultured on various diagnostic plates. The Wizard® Genomic DNA Purification System was used to isolate DNA from any suspect Campylobacter cultures. The purified DNA was used in multiplex PCR and RFLP to determine which species of Campylobacter was present.
(0004016) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 5. |
El Sheikh, A.F., Poret-Peterson, A.T. and Klotz, M.G.
(2008)
Characterization of two new genes, amoR and amoD, in the amo operon of the marine ammonia oxidizer Nitrosococcus oceani ATCC 19707.
Appl. Environ. Microbiol.
74
,
312-318
.
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Notes:
These authors investigated the amo operon of the marine ammonia oxidizer Nitrosococcus oceani. The bacteria were grown at 30°C for 3 weeks in 200-400ml batch cultures in artificial seawater in the dark without shaking. Genomic DNA was isolated from cells in stationary phase using the Wizard® Genomic DNA Purification Kit. The isolated DNA was then used for PCR analysis.
(0003740) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 6. |
Yunis, J.J., García, O., Moreno, S., Pineda, C., Rodriguez, C., Uriarte, I. and Yunis, E.J.
(2008)
Population data on Powerplex 2.1 (FGA, vWA, TPOX, THO1, Penta E, D18S51, D21S11, D3S1358, D8S1179) and Gammastar (D16S539, D7S820, D13S317, D5S818) in a sample of Caucasian-Mestizos from Colombia.
Int. Congr. Ser.
1239
,
207–12
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Notes:
The authors generated population data for unrelated Caucasian-Mestizos from Columbia using the PowerPlex® 2.1 System and the GammaSTR® Multiplex. DNA was isolated from whole blood using the Wizard® Genomic DNA Purification Kit or ReadyAmp™ Genomic DNA Purification System, then amplified per the manufacturer's recommendations. Amplified fragments were detected using a Hitachi FMBIO® II fluorescence imaging system.
(0003856) |
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Products: GammaSTR® Multiplex (Fluorescein) D16S539, D7S820, D13S317, D5S818 | PowerPlex® 2.1 System | ReadyAmp™ Genomic DNA Purification System | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 7. |
Fekete, E., Seiboth, B., Kubicek, C.P., Szentirmai, A., Karaffa, L.
(2008)
Lack of aldose 1-epimerase in Hypocrea jecorina (anamorph Trichoderma reesei): A key to cellulase gene expression on lactose
Proc. Natl. Acad. Sci. U S A
105
,
7141-7146
.
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Notes:
To amplify yeast mutarotase, S. cerevisiae was used, and E. coli strain JM109 (Promega Cat.# L2001) was used for plasmid propagation. Fungal mycelia were harvested by filtration, washed, frozen and ground under liquid nitrogen. Genomic DNA was extracted using the Wizard Genomic DNA Purification System (Promega Cat.# A1120). RNA for hybridization and RT-PCR was extracted from mycelia using the SV Total RNA Isolation System (Promega Cat.# Z3101) and plasmid DNA isolated using the PureYield(TM) Plasmid Midiprep System (Cat.# A2492).
(0003919) |
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Products: JM109 Competent Cells, >107cfu/μg | PureYield™ Plasmid Midiprep System | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 8. |
Flahaut, S., Vinogradov, E., Kelley, K.A., Brennan, S., Hiramatsu, K. and Lee, J.C.
(2008)
Structural and biological characterization of a capsular polysaccharide produced by Staphylococcus haemolyticus.
J. Bacteriol.
190
,
1649–1657
.
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Notes:
The authors wanted to purify and characterize the capsular polysaccharide (CP) produced by Staphylococcus haemolyticus strain JCSC1435. S. haemolyticus strains grown in TSB cultures were harvested, lysed with lysozyme and lysostaphin and genomic DNA isolated using the Wizard® Genomic DNA Purification Kit. The DNA was then used in CP gene PCR. Total RNA was isolated from exponential, postexponential, and stationary phases of S. haemolyticus growth and used in RT-PCR using the Access RT-PCR System.
(0003977) |
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Products: Access RT-PCR Introductory System | Access RT-PCR System | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 9. |
Kim, S., Lee, U.J., Kim, M.N., Lee, E.J., Kim, J.Y., Lee, M.Y., Choung, S., Kim, Y.J. and Choi, Y.C.
(2008)
MicroRNA miR-199a* regulates the MET proto-oncogene and the downstream extracellular signal-regulated kinase 2.
J. Biol. Chem.
283
,
18158–18166
.
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Notes:
To examine the methylation state of DNA of cultured cells, genomic DNA was purified using the Wizard® Genomic DNA Purification Kit. The extracted DNA was restriction enzyme digested and then treated with bisulfite.
(0003978) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 10. |
Hopkins, M.T., Lampi, Y., Wang, T.W., Liu, Z. and Thompson, J.E.
(2008)
Eukaryotic translation initiation factor 5A is involved in pathogen-induced cell death and development of disease.
Plant Physiol.
148
,
479–489
.
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Notes:
Genomic DNA from Arabidopsis thaliana was isolated using the Wizard® Genomic DNA Purification Kit. The extracted DNA was used to amplify the 3´ UTR of AteIF5A-2, A. thaliana translation initiation factor 5A, or the entire gene for creating transgenic plants. Leaf discs from wild-type Arabidopsis were infected with Pseudomonas syringae pv tomato DC3000, a virulent strain regulated by AteIF5A-2, using a syringe. After 24 and 72 hours, 0.4cm leaf discs were fixed, labeled using the DeadEnd™ Fluorometric TUNEL System and stained for AteIF5A-2 protein.
(0003979) |
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Products: DeadEnd™ Fluorometric TUNEL System | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 11. |
Baker, S., Holt, K., van de Vosse, E., Roumagnac, P., Whitehead, S., King, E., Ewels, P., Keniry, A., Weill, F.X., Lightfoot, D., van Dissel, J.T., Sanderson, K.E., Farrar, J., Achtman, M., Deloukas, P. and Dougan, G.
(2008)
High-throughput genotyping of Salmonella enterica serovar Typhi allowing geographical assignment of haplotypes and and pathotypes within an urban District of Jakarta, Indonesia.
J. Clin. Microbiol.
46
,
1741–1746
.
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Notes:
The authors examined strains of Salmonella enterica serovar Typhi isolated from typhoid cases originating in or around Indonesia or from travelers returning from Indonesia to examine if serovar Typhi from this area has a greater level of genetic diversity compared to other countries. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit, diluted to 4 ng/µl and used in locus-specific PCR genotyping.
(0003980) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 12. |
Monje, L., Varayoud, J., Luque, E.H. and Ramos, J.G.
(2007)
Neonatal exposure to bisphenol A modifies the abundance of estrogen receptor alpha transcripts with alternative 5'-untranslated regions in the female rat preoptic area.
J. Endocrinol.
197
,
201–12
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Notes:
The authors investigated the effect of neonatal bisphenol A (BPA) exposure in rats on expression of estrogen receptor α (ERα) transcripts. Alternative ERα transcripts in preoptic area of treated and untreated rats were quantified using real-time RT-PCR. Reverse transcription was performed using 4µg of total RNA, 200pmol random primers and 300 units M-MLV Reverse Transcriptase. Real-time PCR was performed using SYBR® Green I to quantify amplified products. To determine if the changes in BPA-induced ERα transcript expression were caused by DNA methylation, the methylation status of the five ERα promoters was examined by bisulfite modification. Genomic DNA was isolated from rat tissue using the Wizard® Genomic DNA Purification Kit, denatured with NaOH, then treated with hydroquinone and sodium bisulfite. Prior to methylation-specific PCR, DNA was cleaned up using the Wizard® DNA Purification Resin as directed by the manufacturer. PCR products were cleaned up again using the Wizard® SV Gel and PCR Clean-Up System, then subjected to restriction enzyme digestion and agarose gel electrophoresis to reveal methylation-dependent sequence differences.
(0003911) |
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Products: M-MLV Reverse Transcriptase | Random Primers | Set of dATP, dCTP, dGTP, dTTP | Wizard® Genomic DNA Purification Kit | Wizard® SV Gel and PCR Clean-Up System |
| 13. |
Soltani-Bejnood, M., Thomas, S.E., Villeneuve, L., Schwarz, K.T., Hong, C.S. and McKee, B.D.
(2007)
Role of the mod(mdg4) common region in homolog segregation in Drosophila male meiosis.
Genetics
176
,
161–180
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Notes:
In this paper, the authors studied the common region of mod(mdg4) and its role in homolog conjunction during meiosis. Genomic DNA was isolated from adult Drosophila using the Wizard® Genomic DNA Purification Kit and mutations identified by PCR and sequencing of the introns.
(0003576) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 14. |
Lucas, P.M., Claisse, O. and Lonvaud-Funel, A.
(2007)
High frequency of histamine-producing bacteria in enological environment and instability of the phenotype.
Appl. Environ. Microbiol.
74
,
811-817
.
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Notes:
Because of concerns about the consumption of histamine in wine (which makes histamine more potent), the quantity of lactic acid bacteria (LAB), which can produce histamine during winemaking, was determined in 264 samples of red wine from 116 wineries. DNA was isolated from LAB strains grown in De Man Rogosa Sharpe (MRS) broth using the Wizard® Genomic DNA Purification Kit. Glass beads were used to disrupt the cells, and a modified Wizard® Genomic DNA Purification protocol that included PVP treatment was performed. The purified DNA was used in qPCR analysis.
(0003741) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 15. |
Palmer, M.T., Kirkman, R., Kosloff, B.R., Eipers, P.G. and Morrow, C.D.
(2007)
tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation.
J. Virol.
81
,
4397-4404
.
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Notes:
These authors examined how the retrovirus Moloney murine leukemia virus (MuLV) selected tRNA, which is used for primer binding and initiating reverse transcription, by mutating in the primer binding sites (PBS). At various time points, high-molecular-weight DNA was isolated from cells infected with MuLV using the Wizard® Genomic DNA Purification Kit. The purified DNA was amplified using primers to the PBS, sequenced and analyzed by an alignment program.
(0003742) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 16. |
Starkenburg, S.R., Chain, P.S., Sayavedra-Soto, L.A., Hauser, L., Land, M.L., Larimer, F.W., Malfatti, S.A., Klotz, M.G., Bottomley, P.J., Arp, D.J. and Hickey, W.J.
(2006)
Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.
Appl. Environ. Microbiol.
72
,
2050–2063
.
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Notes:
To construct a genome library of the nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255, genomic DNA was isolated from batch cultures using the Wizard® Genomic DNA Purification Kit.
(0003416) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 17. |
Roberti, M., Bruni, F., Polosa, P.L., Gadaleta, M.N. and Cantatore, P.
(2006)
The Drosophila termination factor DmTTF regulates in vivo mitochondrial transcription.
Nucleic Acids Res.
34
,
2109–2116
.
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Notes:
To examine if the depletion of Drosophila transcription termination factor (DmTTF) after RNAi treatment could reduce the gene copy number, genomic DNA was isolated from RNAi-treated and untreated Drosophila embryonic D.Mel-2 cells using the Wizard® Genomic DNA Purification Kit. The mitochondrial ND3 gene and the nuclear H2B histone gene were used as probes for the Xho I-digested, Southern-blotted genomic DNA to compare the treatment groups. The Wizard® SV Gel and PCR Clean-Up System was used to clean up the PCR-amplified probes.
(0003418) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit | Wizard® SV Gel and PCR Clean-Up System |
| 18. |
Oka, M., Rodic, N., Graddy, J., Chang, L.J. and Terada, N.
(2006)
CpG sites preferentially methylated by Dnmt3a in vivo.
J. Biol. Chem.
281
,
9901–9908
.
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Notes:
The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from mouse embryonic stem (ES) cells, embryonic bodies (EB) and adult tissues. The purified genomic DNA was subjected to methylation-sensitive restriction fingerprinting (MSRF; digestion with methylation-sensitive restriction enzymes followed by amplification of 100ng of digested DNA), or 2µg DNA treated with bisulfite.
(0003419) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 19. |
Pomerantsev, A.P., Sitaraman, R., Galloway, C.R., Kivovich, V. and Leppla, S.H.
(2006)
Genome engineering in Bacillus anthracis using Cre recombinase.
Infect. Immun.
74
,
682–693
.
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Notes:
Chromosomal DNA from Gram-positive Bacillus anthracis was isolated using the Wizard® Genomic DNA Purification Kit.
(0003420) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 20. |
Yu, Z., Loehr, C.V., Fischer, K.A., Louderback, M.A., Krueger, S.K., Dashwood, R.H., Kerkvliet, N.I., Pereira, C.B., Jennings-Gee, J.E., Dance, S.T., Miller, M.S., Bailey, G.S. and Williams, D.E.
(2006)
In utero exposure of mice to dibenzo[a,l]pyrene produces lymphoma in the offspring: role of the aryl hydrocarbon receptor.
Cancer Res.
66
,
755–762
.
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Notes:
To examine the role of aryl hydrocarbon receptor (AHR) in dibenzo[a,l]pyrene (DBP) tumor development, AHR-responsive and nonresponsive murine fetuses were treated in utero with DBP. Genomic DNA was isolated from lymphomas using the Wizard® Genomic DNA Purification Kit. Two microliters of the purified DNA was PCR-amplified for analysis of the Ki-ras locus, where mutations are associated with tumor susceptibility.
(0003421) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 21. |
Tan, A., Westerman, D., McArthur, G.A., Lynch, K., Waring, P. and Dobrovic, A.
(2006)
Sensitive detection of KIT D816V in patients with mastocytosis.
Clin. Chem.
52
,
2250–2257
.
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Notes:
The authors wanted to develop a more sensitive assay to detect codon 816 pathogenic variations in people diagnosed with systemic mastocytosis. The Wizard® Genomic DNA Purification Kit was used to isolate DNA from peripheral blood and bone marrow aspirate samples. To extract DNA from 2–5 micron, paraffin-embedded samples of bone marrow trephine, skin, spleen or liver, the tissues were digested with Proteinase K for four days at 56°C prior to DNA purification using the Magnesil® Genomic Fixed Tissue System. The isolated DNA was subjected to two assays: enriched sequencing of mutant alleles (ESMA) after BsmAI restriction enzyme digestion, and allele-specific competitive blocker PCR (ACB-PCR).
(0003575) |
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Products: MagneSil® Genomic, Fixed Tissue System | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 22. |
Heg, D., Bergmuller, R., Bonfils, D., Otti, O., Bachar, A., Burri, R., Heckel, G. and Taborskya, M.
(2006)
Cichlids do not adjust reproductive skew to the availability of independent breeding options.
Behav. Ecol.
17
,
419–429
.
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Notes:
These authors sought to determine whether helpers in cooperatively breeding species reproduced or if all offspring were from the breeders. They created 32 breeding groups and tested the possibilities of reproductive skew under various conditions. Genomic DNA from breeders and helpers was isolated from ethanol-preserved 1–2 mm2 finclip samples or from whole offspring using the Wizard® Genomic DNA Purification Kit. Purified DNA was resuspended in 50µl of DNA Rehydration Solution and analyzed by PCR using seven microsatellite primer pairs.
(0003679) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 23. |
Jacobs, M.E., DeSouza, L.V., Samaranayake, H., Pearlman, R.E., Siu, K.W., and Klobutcher, L.A.
(2006)
The Tetrahymena thermophila phagosome proteome.
Eukaryot. Cell.
5
,
1990–2000
.
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Notes:
The authors wanted to learn more about the proteins that comprise the Tetrahymena
phagosome proteome. Proteins were isolated from phagosome preparations, denatured by adding 5mM dithiothreitol and heating to 60°C for 1 hour. The samples were cooled to room temperature and alkylated by incubation with 10mM iodoacetamide for 1 hour protected from light. Sequencing Grade Modified Trypsin, at a 1:20 concentration (wt/wt) with an equal volume of 50mM ammonium bicarbonate, was added to the sample and incubated overnight at 37°C. Each sample was analyzed by two-dimensional liquid chromatography–tandem mass spectrometry (LC-MS/MS). Whole-cell Tetrahymena DNA was isolated using the Wizard® Genomic DNA Purification Kit protocol for isolating genomic DNA from plant tissue omitting the use of liquid nitrogen with mortar and pestle. The isolated DNA was then used for restriction digestion, ligation and sequencing.
(0003680) |
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Products: Sequencing Grade Modified Trypsin | Sequencing Grade Modified Trypsin, Frozen | Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 24. |
Rossi, F., Morlacchini, M., Fusconi, G., Pietri, A., Mazza, R., and Piva, G.
(2005)
Effect of Bt corn on broiler growth performance and fate of feed-derived DNA in the digestive tract.
Poult. Sci.
84
,
1022–1030
.
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Notes:
These authors studied the effect of transgenic Bacillus thuringiensis (Bt) corn feed containing the Cry1A gene on broiler chicken performance. They also analyzed the degradation of the Cry1A(b) transgene in the digestive tract of the chickens. Blood and samples from cecum, jejunum, gizzard, and crop were collected from ten broilers per treatment (fed Bt corn versus near isogenic corn). Genomic DNA was isolated from blood samples and the intestinal contents of the crop and gizzard using the Wizard® Genomic DNA Purification Kit. Fifty nanograms of purified DNA were then used in PCR analysis.
(0003678) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |
| 25. |
van Schaik, W., Tempelaars, M.H., Wouters, J.A., de Vos, W.M. and Abee, T.
(2004)
The alternative sigma factor σB of Bacillus cereus: Response to stress and role in heat adaptation.
J. Bacteriol.
186
,
316–325
.
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Notes:
The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from Bacillus cereus. The isolated DNA was used in PCR to create probes for Northern blotting Bacillus cereus total RNA to detect rsbV and orf4 gene transcripts.
(0003100) |
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Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit |