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| 1. |
Chung, R.T., He, W., Saquib, A., Contreras, A.M., Xavier, R.J., Chawla, A., Wang, T.C., and Schmidt, E.V.
(2001)
Hepatitis C virus replication is directly inhibited by IFN-alpha in a full-length binary expression system.
Proc. Natl. Acad. Sci. U S A
98
,
9847-9852
.
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Notes:
The authors use a T7 polymerase-driven full-length hepatitis C viral cDNA plasmid to reproduce the early steps of the HCV life cycle in vivo. The positive control plasmid used in these experiments contained a T7 promoter flanking the β-galactosidase gene. Expression of HCV proteins and the control β-galactosidase protein in CV-1 and HepG2 cells was detected by Western blot analysis. The Anti-β-Galactosidase mAb was used at a 1:5,000 dilution.
(0002417) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |
| 2. |
Kedersha, N.L., Gupta, M., Li, W., Miller, I., and Anderson P.
(1999)
RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules.
J. Cell Biol.
147
,
1431-42
.
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Notes:
The authors examine the colocalization of several RNA-binding proteins and poly(A)(+) RNA at mammalian stress granules, cytoplasmic foci that result from an environmental stress. The effects of wild-type and mutant eIF-2 on stress granule formation was also examined. COS cells were transiently transfected with a ß-galactosidase reporter plasmid and plasmids encoding wild-type and mutant eIF-2. Cell lysates were subjected to Western blot analysis with the Anti–ß-Galactosidase mAb (1:2000 dilution) to monitor ß-galactosidase expression.
(0002419) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |
| 3. |
Wang, L., Elliott, M., and Elliott T.
(1999)
Conditional stability of the HemA protein (glutamyl-tRNA reductase) regulates heme biosynthesis in Salmonella typhimurium.
J. Bacteriol.
181
,
1211-1219
.
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Notes:
Regulation of glutamyl-tRNA reductase (HemA), which catalyzes the first committed step in heme synthesis appears to be through stabilization of the protein rather than transcriptional regulation. Western blot analysis of HemA and a HemA-LacZ fusion protein revealed that the HemA protein accumulates under low heme conditions. The Anti-B-Galactosidase mAb was used to detect the HemA-LacZ fusion protein in Salmonella typhimurium and Escherichia coli. The same antibody was also used for immunoprecipitation of the HemA-LacZ fusion protein.
(0002420) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |