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| 1. |
Heerssen, H.M., Pazyra, M.F. and Segal, R.A.
(2004)
Dynein motors transport activated Trks to promote survival of target-dependent neurons.
Nat. Neurosci.
7
,
596-604
.
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Notes:
This paper describes an assay to test BDNF dissociation from FluoSpheres (amine-modified microspheres, 1μM diameter; Molecular Probes). BDNF was covalently attached to the microspheres. Release of BDNF was tested in supernatants from the BDNF-microspheres in PBS, in complete media, and in culture with rat Dorsal root ganglia. Promega’s BDNF Emax® ImmunoAssay System was used to determine the amounts of BDNF in these samples. The DeadEnd™ Fluorometric TUNEL System was used to examine rat Dorsal root ganglia cells that had been transfected with either dynamitin or β-Galactosidase. In the TUNEL experiments, cells were fixed in 4% paraformaldehyde and counterstained with DAPI. Cells were also stained with Promega’s Anti-β-Galactosidase, Purified Monoclonal Antibody. For these immunocytochemical stains, a 1:500 dilution of Anti-β-Galactosidase antibody and a 1:1,500 dilution of a secondary antibody conjugated to Alexa Fluor-488 (Molecular Probes) were used. The stains were visualized by fluorescence microscopy.
(0003055) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody | BDNF Emax® ImmunoAssay System | DeadEnd™ Fluorometric TUNEL System |
| 2. |
Jarriault, S. and Greenwald, I.
(2002)
Suppressors of the egglaying defective phenotype of sel-12 presenilin mutants implicate the CoREST corepressor complex in LIN-12/Notch signaling in C. elegans.
Genes Dev.
16
,
2713–2728
.
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Notes:
The authors of this study identified pi cells based on the specific expression of a lin-11::lac-Z transgene, detected using the Anti-β-Galactosidase mAb. In these experiments, whole C. elegans worms were mounted on slides with antifade (Molecular Probes) and counterstained with DAPI.
(0003198) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |
| 3. |
Wijgerde, M., McMahon, J.A., Rule, M. and McMahon, A.P.
(2002)
A direct requirement for Hedgehog signaling for normal specification of all ventral progenitor domains in the presumptive mammalian spinal cord.
Genes Dev.
16
,
2849–2864
.
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Notes:
In this study, the authors used the Anti-β-Galactosidase mAb to distinguish modified embryonic stem cells from wild-type host cells in mouse chimeras. During the creation of the chimeras, β-galactosidase was used as a marker for genetically modified stem cells. In these experiments whole embryos were fixed in 4% paraformaldehyde with 30% sucrose and mounted with OCT medium. The Anti-β-Galactosidase mAb was used at a dilution of 1:1000.
(0003199) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |
| 4. |
Zhang, F., White, R.L., and Neufeld, K.L.
(2000)
Phosphorylation near nuclear localization signal regulates nuclear import of adenomatous polyposis coli protein.
Proc. Natl. Acad. Sci. U S A
97
,
12577-82
.
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Notes:
Two potential nuclear localization signals in the adenomatous polyposis coli gene were scrutinized by inserting these signals into a β-galactosidase expression vector. Mouse L cells and Cos7 cells were transfected with the β-galactosidase construct and immunostained to monitor nuclear transport. The Anti-β-Galactosidase mAb (1:1,000 dilution) and a goat anti-mouse IgG-FITC were used to immunostain the transfected cells.
(0002423) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |
| 5. |
Hou, L., Panthier, J.J., and Arnheiter, H.
(2000)
Signaling and transcriptional regulation in the neural crest-derived melanocyte lineage: Interactions between KIT and MITF.
Development
127
,
5379-89
.
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Notes:
Knockout mice deficient in the tyrosine kinase receptor Kit, which is critical for the development of melanocytes from neural crest-derived precursor cells, were generated. The Kit encoding region was replaced in these mice with the LacZ gene, a convenient marker for Kit expression. Kit-LacZ homozygous mices were identified by PCR using Promega's Taq DNA Polymerase to amplify a 800bp band corresponding to LacZ and to confirm the absence of the 148bp band of Kit. Primary neural crest cell cultures from knockout mouse embryos were immunostained with the Anti-β-Galactosidase mAb to localize Kit expression. Cells were fixed in 4% paraformaldhyde and permeabilized with 0.1% Triton®-X-100 prior to immunostaining.
(0002422) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody |
| 6. |
Lewis, S.E., Mannion, R.J., White, F.A., Coggeshall, R.E., Beggs, S., Costigan, M., Martin, J.L., Dillmann, W.H. and Woolf, C.J.
(1999)
A role for HSP27 in sensory neuron survival.
J. Neurosci.
19
,
8945-8953
.
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Notes:
The 2.5S Nerve Growth Factor was used to culture rat dorsal root ganglia neurons. The primary rat cells were infected with an adenovirus expressing E. coli β-galactosidase (lacZ) and the enzyme was detected by immunocytochemistry with the Anti-β-Galactosidase mAb. Primary cells infected with either an HSP27 adenovirus or LacZ adenovirus were withdrawn from NGF and survival assayed 48 hours later with the CellTiter 96® Non-Radioactive Cell Proliferation Assay. Only those cells expressing HSP27 showed some survival and percent survival was dependent upon the multiplicity of infection.
(0000788) |
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Products: Anti-β-Galactosidase, Purified Monoclonal Antibody | CellTiter 96® Non-Radioactive Cell Proliferation Assay | mNGF, 2.5S |