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1. Xie, Y., and Varshavsky, A. (2001) RPN4 is a ligand, substrate, and transcriptional regulator of the 26S proteasome: a negative feedback circuit. Proc. Natl. Acad. Sci. U S A 98 , 3056-61 .
  Notes: The RPN4 protein is involved in the regulation of levels of proteasomal subunits in Saccharomyces cerevisiae. The protein was found to be short-lived by fusing the RPN4 degradation signal to the long-lived β-galactosidase protein. Levels of this fusion protein were quantitated by immunoprecipitation of equal amounts of TCA-insoluble 35S labeled protein using the Anti-β-Galactosidase mAb. Yeast cells were lysed by vortexing with glass beads in lysis buffer (1% Triton® X-100/0.15 M NaCl/1 mM EDTA/50 mM Na-Hepes, pH 7.5) containing protease inhibitors prior to immunoprecipitation. (0002446)
 
  Products: Anti-β-Galactosidase, Purified Monoclonal Antibody
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