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| 1. |
Vijayan K.V., Liu Y., Dong J.F. and Bray P.F.
(2003)
Enhanced activation of mitogen-activated protein kinase and myosin light chain kinase by the Pro33 polymorphism of integrin beta 3.
J. Biol. Chem.
278
,
3860-3867
.
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Notes:
The role of integrin alpha(IIb)beta(3) in focal adhesion kinase activation and MAPK signaling was studied using Chinese hamster ovary and human kidney 293 cell lines expressing either the Leu(33) or Pro(33) isoform of beta(3). Compared with Leu(33) cells, Pro(33) cells demonstrated substantially greater activation of ERK2 (but not MAPK family members JNK and p38) upon adhesion to immobilized fibrinogen (but not fibronectin), and upon integrin cross-linking. ERK2 activation was mediated through MAPK kinase and required phosphoinositide 3-kinase signaling and an intact actin cytoskeleton. Levels of activated MAPK family members ERK1 and 2, JNK, and p38 were assessed by western blotting using Anti-ACTIVE® MAPK (1:5000 dilution), Anti-ACTIVE® JNK (1:5000 dilution), and Anti-ACTIVE® p38 (1:1000 dilution) pAbs. Anti-ERK 1/2 pAb was used at a 1:5000 dilution as a control for total protein amounts loaded on the blots.
(0002789) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) | Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) | Anti-ACTIVE® p38 pAb, Rabbit, (pTGpY) | Anti-ERK 1/2 pAb, Rabbit |
| 2. |
Le'Negrate, G., Rotagno, R., Auberger, P., Rossi, B., Hofman, P.
(2003)
Down regulation of caspases and Fas ligand expression, and increased lifespan of neutrophils after transmigration across intestinal epithelium
Cell Death Differ.
10
,
153-162
.
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Notes:
Anti-ACTIVE® JNK pAb was used in immunoblot analysis of human polymorphonuclear leukocyte protein lysates.
(0002665) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) |
| 3. |
Ciesielski-Treska, J., Ulrich, G., Chasserot-Golaz, S., Zwiller, J., Revel, M.O., Aunis, D., and Bader M.F.
(2001)
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia.
J. Biol. Chem.
276
,
13113-13120
.
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Notes:
The neurotoxic effects of chromogranin A activated microglia in neurodegenerative diseases was examined. Rat neurons were grown in the presence or absence of conditioned media from chromogranin A treated or untreated microglia. DNA fragmentation in apoptotic neurons was detected using the DeadEnd™ Fluorometric TUNEL System. The levels of dually phosphorylated, active JNK in these cells was determined by Western blot analysis using the Anti-ACTIVE® JNK pAb. A pan JNK antibody was used to normalize for total JNK protein on the Western blots.
(0002378) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) |
| 4. |
Rumora, L., Shaver, A., Zanic-Grubisic, T., and Maysinger, D.
(2001)
Differential regulation of JNK activation and MKP-1 expression by peroxovanadium complexes.
Neurochem. Int.
38
,
341-347
.
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Notes:
The effect of bisperoxovanadium complexes, known protein tyrosine phosphatase inhibitors, on the activation of JNK was examined in a number of cell lines, including HeLa, PC12 and OVCAR-3. The level of dually phosphorylated (activated) JNK was determined by Western blot analysis using a 1:1000 dilution of Promega's Anti-ACTIVE® JNK pAb.
(0002381) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) |
| 5. |
Terstegen, L., Gatsios, P., Bode, J.G., Schaper, F., Heinrich, P.C., and Graeve, L.
(2000)
The inhibition of interleukin-6-dependent STAT activation by mitogen-activated protein kinases depends on tyrosine 759 in the cytoplasmic tail of glycoprotein 130.
J. Biol. Chem.
275
,
18810-18817
.
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Notes:
The effect of suppressor of cytokine signaling (SOCS) expression on the Jak/STAT, MAPK, JNK, and p38 signaling pathways was examined in HepG2, Cos-7, and NIH 3T3 cells. Both PMA and bFGF (Promega) resulted in a rapid upregulation of SOCS-3 expression. MAPK, JNK, and p38 activation was monitored by Western blot analysis using the Anti-ACTIVE® MAPK Anti-ACTIVE® JNK pAb, and the Anti-ACTIVE® p38 pAb. Total levels of active and inactive MAPK protein was determined using the Anti-ERK 1/2 pAb, Rabbit.
(0002380) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) | Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) | Anti-ACTIVE® p38 pAb, Rabbit, (pTGpY) |
| 6. |
Paumelle, R., Tulasne, D., Leroy, C., Coll, J., Vandenbunder, B., and Fafeur, V.
(2000)
Sequential activation of ERK and repression of JNK by scatter factor/hepatocyte growth factor in madin-darby canine kidney epithelial cells.
Mol. Biol. Cell
11
,
3751-3763
.
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Notes:
The authors characterize the cell signaling pathways triggered by the multifunctional growth factor scatter factor/hepatocyte growth factor. In Madin-Darby Canine Kidney epithelial cells SF/HGF induces phosphorylation of MAPK while stimulating weakly and then repressing phosphorylation of JNK. The Anti-ACTIVE® MAPK pAb and Anti-ACTIVE® JNKpAb were used to quantitate the level of activation of the MAPK and JNK signaling pathways by Western blot analysis.
(0002379) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) | Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) |
| 7. |
Burns, K., Clatworthy, J., Martin, L., Martinon, F., Plumpton, C., Maschera, B., Lewis, A., Ray, K., Tschopp, J., and Volpe, F.
(2000)
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor.
Nat. Cell Biol.
2
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346-51
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Notes:
Activation of JNK in response to interleukin-1 treatment of 293 cells transfected with a type I IL-1 receptor expression plasmid was quantitated. Extracts of 293T cells were subjected to Western blot analysis with the Anti-ACTIVE® JNK pAb (1:5000 dilution) to detect the active, dually phosphorylated forms of JNK.
(0002459) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) |
| 8. |
Sawamoto, K., Winge, P., Koyama, S., Hirota, Y., Yamada, C., Miyao, S., Yoshikawa, S., Jin, M.H., Kikuchi, A., and Okano, H.
(1999)
The Drosophila Ral GTPase regulates developmental cell shape changes through the Jun NH(2)-terminal kinase pathway.
J. Cell Biol.
146
,
361-372
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Notes:
A Ral GTPase, which regulates developmental changes through inhibition of the JNK pathway, is identifed in Drosophila. Inhibition of the JNK pathway in the presence of a constitutively active Ral protein was examined by Western blot using Promega's Anti-ACTIVE® JNK pAb. Drosophila S2 cells were treated with 500 mM sorbitol for 5 min, lysed, subjected to SDS-PAGE, and transferred to nylon membranes. The membranes were incubated with Anti-ACTIVE® JNK to detect the dually phosphorlyated (active) form of JNK.
(0002385) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) |