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Fujii, R., Yamashita, S., Hibi, M., and Hirano, T
(2000)
Asymmetric p38 activation in zebrafish: Its possible role in symmetric and synchronous cleavage.
J. Cell Biol.
150
,
1335-48
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Notes:
Early steps of embryogenesis are characterized by a series of symmetric and synchronous cell divisions. The authors show that p38 MAPK is asymmetrically activated on one side of early zebrafish embryos. Dually phosphorylated p38 protein was detected in lysates prepared from one-cell stage embryos by Western blot analysis with the Anti-ACTIVE® p38 pAb. Immunohistochemistry to detect active p38 was also performed on embryos fixed in 4% paraformaldehyde, permeabilized in methanol, and stained with the Anti-ACTIVE® p38 pAb at a 1:50 dilution. Active p38 levels were also determined by Western blot analysis in 293T cells expressing p38, MKK3, JNK, or human constitutively active form of zebrafish MKK4.
(0002406) |
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Products: Anti-ACTIVE® p38 pAb, Rabbit, (pTGpY) |