|
| 1. |
Matsumoto, Y., Maller, J.L.
(2002)
Calcium, calmodulin, and CaMKII requirement for initiation of centrosome duplication in Xenopus egg extracts
Science
295
,
499-502
.
|
| |
Notes:
CaMKII(281-309) was added to metaphase-arrested extracts. After adding calcium, the extracts were incubated at room temperature. Anti-ACTIVE® CaMKII pAb and Anti-ACTIVE® qualified HRP secondary antibodies were used to probe immunoblots for phospho-T286 CaMKIIα.
(0002590) |
| |
 |
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) | Donkey Anti-Rabbit IgG (H+L), HRP |
| 2. |
Taha, S., Hanover, J.L., Silva, A.J. and Stryker, M.P.
(2002)
Autophosphorylation of αCaMKII is required for ocular dominance plasticity.
Neuron
36
,
483–491
.
|
| |
Notes:
Anti-ACTIVE® CaM KII pAb was used to detect phosphorylated CAM KII by immunohistochemistry and Western blot analysis in tissues isolated from transgenic mice. A 1:200 dilution of the Anti-ACTIVE® CaM KII pAb was used in conjunction with an immunoperoxidase detection kit for immunohistochemical analysis. For Western blot analysis, a 1:5000 dilution of the antibody was used.
(0003202) |
| |
|
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) |
| 3. |
Blank, T., Nijholt, I., Eckart, K. and Spiess, J.
(2002)
Priming of long-term potentiation in mouse hippocampus by corticotropin-releasing factor and acute stress: implications for hippocampus-dependent learning.
J. Neurosci.
22
,
3788-3794
.
|
| |
Notes:
This paper studied the effects of both acute stress and Corticotropin-releasing factor (CRF) on hippocampus-dependent learning and on long-term synaptic plasticity in the mouse hippocampus. In particular, the roles of PKC and CaMKII in the regulation of hippocampal long-term synaptic plasticity and in the performance of mice in a hippocampus-dependent learning task were studied. The level of active CaM KII in mouse hippocampal extracts was investigated by western blotting using the Anti-ACTIVE™ CaM KII antibody.
(0002765) |
| |
|
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) |
| 4. |
Lengyel, I., Cammarota, M., Brent, V.A., and Rostas, J.A.
(2001)
Autonomous activity and autophosphorylation of CAMPK-II in rat hippocampal slices: Effects of tissue preparation.
J. Neurochem.
76
,
149-54
.
|
| |
Notes:
The authors show how certain common tissue handling techniques, such as equilibration for 1-2 hours in a physiological medium and freezing, can dramatically affect the level of autonomous CaM KII activity in hippocampal tisuse, leading to potential artefacts in data. Activation of Cam KII was monitored by Western blot analysis using the Anti-ACTIVE® CaM KII pAb primary antibody.
(0002408) |
| |
|
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) |
| 5. |
Wang, H., Yang, B., Zhang, Y., Han, H., Wang, J., Shi, H., and Wang, Z.
(2001)
Different subtypes of alpha1-adrenoceptor modulate different K+ currents via different signaling pathways in canine ventricular myocytes.
J. Biol. Chem.
276
,
40811-6
.
|
| |
Notes:
The signal transduction mechanisms of adrenoreceptors in regulating K+ currents in isolated canine ventricular myocytes and human embryonic kidney (HEK 293) cells were examined. The CamKII pathway was monitored in the presence or absence of a specific inhibitor CaM KII inhibitor by Western blot using the Anti-ACTIVE® CaM KII pAb.
(0002407) |
| |
|
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) |
| 6. |
Pak, J.H., Huang, F.L., Li, J., Balschun, D., Reymann, K.G., Chiang, C., Westphal, H., and Huang, K.P
(2000)
Involvement of neurogranin in the modulation of calcium/calmodulin-dependent protein kinase II, synaptic plasticity, and spatial learning: a study with knockout mice.
Proc. Natl. Acad. Sci. U S A
97
,
11232-7
.
|
| |
Notes:
Neurogranin deficient mice did not exhibit developmental or neuroanatomical abnormalities but had deficits in learning and memory. The levels of phosphorylated CaM KII in hippocampal extracts was determined by quantitative immunoblotting using the Anti-ACTIVE® CamKII pAb.
(0002410) |
| |
|
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) |
| 7. |
Kuhl, M., Sheldahl, L.C., Malbon, C.C., and Moon, R.T.
(2000)
Ca2+/Calmodulin-dependent Protein Kinase II Is Stimulated by Wnt and Frizzled Homologs and Promotes Ventral Cell Fates in Xenopus
J. Biol. Chem.
275(17)
,
12701-12711
.
|
| |
Notes:
Xenopus embryos were split into dorsal and ventral halves at stage 7. The total CaM KII was immunoprecipitated then blotted with the Anti-ACTIVE® CaM KII pAb. Both halves had activated CaM KII but the ventral portion displayed a greater increase in phosphorylation. Stripping and reprobing the membranes with a normal CaM KII antibody demonstrated that equal amounts of CaM KII were present in the two samples.
(0000070) |
| |
|
| |
Products: Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) |