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| 1. |
Xiao, Y., Zhong, Y., Greene, W., Dong, F. and Zhong, G.
(2004)
Chlamydia trachomatis infection inhibits both Bax and Bak activation induced by staurosporine.
Infect. Immun.
72
,
5470-5474
.
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Notes:
The DeadEnd™ Fluorometric TUNEL System was used to label apoptotic Hela cells that were infected with C. trachomatis for 40 hours before treatment with 2μg/ml Staurosporine for an additional 5 hours. Cultures were co-stained with either Anti-ACTIVE® Caspase-3 pAb or Anti-Cytochrome c mAb. Cy®3-conjugated goat anti-rabbit or -mouse IgG was used as a secondary labeling antibody and the cells were visualized by confocal microscopy.
(0003252) |
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Products: Anti-ACTIVE® Caspase-3 pAb | Anti-Cytochrome c mAb | DeadEnd™ Fluorometric TUNEL System |
| 2. |
Yu, A., McMaster, C.R., Byers, D.M., Ridgway, N.D. and Cook, H.W.
(2003)
Stimulation of phosphatidylserine biosynthesis and facilitation of UV-induced apoptosis in Chinese hamster ovary cells overexpressing phospholipid scramblase 1.
J. Biol. Chem.
278 (11)
,
9706-9714
.
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Notes:
Researchers used the Anti-ACTIVE® Caspase-3 polyclonal antibody to immunocytochemically stain transiently transfected CHO-K1 (Chinese Hamster) cells overexpressing PLSCR1 or PLSCR2. Confocal microscopy, in conjuction with an immunofluorescent Alexa fluor 488 secondary antibody, was used to visualize samples. The authors provide details of the staining procedure. They used a 1:500 dilution of the Anti-ACTIVE® Caspase-3 pAb and counterstained samples with propidium iodide. Representative microscopic images were displayed as well as a graphical depiction of the percent of active caspase-3-positive cells.
(0002832) |
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Products: Anti-ACTIVE® Caspase-3 pAb |
| 3. |
Wyttenbach, A., Sauvageot, O., Carmichael, J., Diaz-Latoud, C., Arrigo, A.P. and Rubinsztein, D.C.
(2002)
Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Hum. Mol. Genet.
11
,
1137-1151
.
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Notes:
Anti-ACTIVE® Caspase-3 antibody was used in immunocytochemical analysis of human neuroblastoma (SK-N-SH) cells transiently transfected with various vectors and an expression vector expressing huntingtin exon 1 (httEx1) containing 103 glutamines fused to enhanced green fluorescent protein (EGFP). The authors also used Promega’s SB 203580 MAP kinase homologue, p38α, p38β and p38β2 inhibitor in both COS-7 and SK-N-SH huntingtin exon 1-transfected cell cultures. Decreased nuclear fragmentation was reported when 1 or 10μM SB 203580 inhibitor was added to the transfected cell cultures.
(0002828) |
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Products: Anti-ACTIVE® Caspase-3 pAb | SB 203580 |
| 4. |
Bezzi, P., Domercq, M., Brambilla, L., Galli, R., Schols, D., De Clercq, E., Vescovi, A., Bagetta, G., Kollias, G., Meldolesi, J., and Volterra, A.
(2001)
CXCR4-activated astrocyte glutamate release via TNFalpha: amplification by microglia triggers neurotoxicity.
Nat. Neurosci.
4
,
702-710
.
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Notes:
The apoptotic nature of neuron cell death via a chemokine-activated cell–cell communication system involving microglia was characterized. Hippocampal pyramidal neurons were obtained from embryonic day 17 rat brain. Cells were exposed to gp120IIIB and stained for neuronal death by apoptosis using the DeadEnd Colorimetric TUNEL System. Neuronal death was also detected by immunocytochemistry using the Anti-ACTIVE® Caspase-3 pAb (1:250 dilution).
(0002352) |
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Products: Anti-ACTIVE® Caspase-3 pAb | DeadEnd™ Colorimetric TUNEL System |