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| 1. |
Paroni, G., Mizzau, M., Henderson, C., Del Sal, G., Schneider, C. and Brancolini, C.
(2004)
Caspase-dependent regulation of histone deacetylase 4 nuclear-cytoplasmic shuttling promotes apoptosis.
Mol. Biol. Cell
15
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1804-2818
.
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Notes:
In this study, the Anti-PARP p85 Fragment polyclonal antibody was used to confirm apoptosis in human IMR90 and MCF-7 cells by Western blotting lysates from UV- treated cells. The Anti-Cytochrome c monoclonal antibody was also used to immunocytochemically stain IMR90 cells that were transiently transfected with various mutant histone deacetylase-4 (HDAC4)-expressing constructs. Data from these experiments was expressed as the percent of cells showing cytochrome c staining in the cytosol. The researchers also mention using a TNT® Coupled Reticulocyte Lysate System to express and label various histone deacetylases (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, and HDAC6). The translation products were then used in in vitro cleavage assays with recombinant caspase-2 or caspase-3.
(0003174) |
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Products: Anti-Cytochrome c mAb | Anti-PARP p85 Fragment pAb |
| 2. |
Henderson, C., Mizzau, M., Paroni, G., Maestro, R., Schneider, C. and Brancolini, C.
(2003)
Role of caspases, Bid, and p53 in the apoptotic response triggered by histone deacetylase inhibitors trichostatin-A (TSA) and suberoylanilide hydroxamic acid (SAHA).
J. Biol. Chem.
278
,
12579–12589
.
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Notes:
The Anti-PARP p85 Fragment polyclonal antibody was used to detect cleaved p85 fragment PARP on Western blots of lysates from caspase-3 deficient human MCF-7 cells, and from cell lines MCF-7/C3WT or MCF-7/C3Cl with rescued caspase-3 expression. Anti-PARP p85 Fragment polyclonal antibody was also used on Western blots of lysates from stably transfected IMR90 human fibroblasts. A variety of treatments were used for inducing apoptosis including serum-free media, colchicine, daunorubicin, TSA (Trichostatin-A), and a compound called ET1 8OCH3. The researchers also used the Anti-Cytochrome c monoclonal antibody to immunocytochemically stain MCF-7/NEO cells.
(0003173) |
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Products: Anti-Cytochrome c mAb | Anti-PARP p85 Fragment pAb |
| 3. |
Mejillano, M., Yamamoto, M., Rozelle, A.L., Sun, H.-Q., Wang, X., and Yin, H.L.
(2001)
Regulation of apoptosis by phosphatidylinositol 4,5-bisphosphate inhibition of caspases, and caspase inactivation of phosphatidylinositol phosphate 5-kinases
J. Biol. Chem.
276(3)
,
1865-1872
.
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Notes:
The Anti-PARP p85 Fragment pAb was used to assess apoptosis in human HEK293 cells transfected and overexpressing phosphatidylinositol phosphate 5-kinase and/or caspase-9. Less cleaved PARP was observed by Western blotting of extracts of cells expressing the kinase in addition to the caspase-9.
(0000019) |
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Products: Anti-PARP p85 Fragment pAb |
| 4. |
Salvucci, O., Carsana, M., Bersani, I., Tragni, G., and Anichini A.
(2001)
Antiapoptotic role of endogenous nitric oxide in human melanoma cells.
Cancer Res.
61
,
318-326
.
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Notes:
The authors find that constitutive production of endogenous NO has an antiapoptotic function in human melanoma cells but not in normal melanocytes. To monitor apoptosis, Western blots were carried out with the Anti-PARP p85 Fragment pAb to monitor PARP cleavage. Melanoma cell extracts (10ug) were separated on 8% polyacrylamide minigels, and transferred to a PVDF membrane. Membranes were incubated with a 1:1000 dilution of the Anti-PARP p85 Fragment pAb, washed, and incubated with a biotinylated-anti rabbit IgG. Detection involved a streptavidin-horseradish peroxidase conjugate and a chemiluminescence substrate. Jurkat cells treated for 6 h with anti-FAS mAb were used as positive controls for Western blot detection of PARP cleavage
(0002354) |
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Products: Anti-PARP p85 Fragment pAb |
| 5. |
Bushell, M., Wood, W., Clemens, M.J., Morley, S.J.
(2000)
Changes in integrity and association of eukaryotic protein synthesis initiation factors during apoptosis.
Eur. J. Biochem.
267
,
1083-1091
.
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Notes:
The human BJAB Burkitt's lymphoma cell line was treated with 150ng/ml anti-Fas mAb (clone CH-11) for 4hr. Cell extracts were prepared and analyzed for cleavage products of translation initiation factors. To demonstrate that caspases were active and cleaving known substrates, the extracts were also analyzed for PARP cleavage with the Anti-PARP p85 Fragment pAb. PARP cleavage products were only obtained from cells treated with the anti-Fas mAb. No PARP cleavage was detected when the cells were treated with the caspase inhibitor Z-VAD-FMK concurrently with anti-Fas treatment.
(0001375) |
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Products: Anti-PARP p85 Fragment pAb |
| 6. |
Berneburg, M., Lowe, J.E., Nardo, T., Araujo, S., Fousteri, M.I., Green, M.H.L., Krutmann, J., Wood, R.D., Stefanini, M. and Lehmann, A.R.
(2000)
UV damage causes uncontrolled DNA breakage in cells from patients with combined features of XP-D and Cockayne syndrome.
EMBO J.
19
,
1157-1166
.
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Notes:
Primary human fibroblasts from normal and DNA-repair -deficient individuals were treated with UVB radiation for up to 72hr. Fibroblasts from Xeroderma pigmentosum patients all demonstrated cleavage of PARP at 72 hours. Normal fibroblasts had no detectable PARP cleavage. The results correlated well with TUNEL data. The cleavage of PARP was detected by Western blotting of cell extracts with the Anti-PARP p85 Fragment pAb. Excellent detail is provided. Human HL60 cells treated with 150mM campothecin for 4hr were used as positive controls.
(0001440) |
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Products: Anti-PARP p85 Fragment pAb |