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| 1. |
Learish, R.D., Bruss, M.D., and Haak-Frendscho, M.
(2000)
Inhibition of mitogen-activated protein kinase kinase blocks proliferation of neural progenitor cells.
Brain Res. Dev. Brain Res.
122
,
97-109
.
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Notes:
A primary cell line from rat subventricular zone which remains undifferentiated over time was developed as a model for MAPK activation. Cell were stimulated with 20 ng/ml bFGF and 20 ng/ml EGF (Promega) to activate MAPK. The MAPK pathway was inhibited with either U0126 (Promega) or PD98059. Immunocytochemistry was performed with the Anti-ERK 1/2 pAb (1:100 dilution) to detect both active and inactive forms of MAPK proteins and with the Anti-ACTIVE™ MAPK pAb (1:100 dilution) to specifically detect the dually phosphorylated, active forms of MAPK. Cells were also immunostained with the neuron specific marker Anti-III-tubulin mAb (0.5 µg/ml). Cell proliferation was monitored with the CellTiter 96® AQueous One Solution Cell Proliferation Assay System. Apoptosis within the cell population was monitored using the DeadEnd™ Fluorometric TUNEL System.
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Products: Anti-βIII Tubulin mAb | Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) | Anti-ERK 1/2 pAb, Rabbit | CellTiter 96® AQueous One Solution Cell Proliferation Assay | DeadEnd™ Fluorometric TUNEL System | Donkey Anti-Rabbit IgG (H+L), HRP | MEK Inhibitor U0126 | RQ1 RNase-Free DNase |