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| 1. |
Moreno-Flores M.T., Lim F., Martin-Bermejo M.J., Diaz-Nido J., Avila J. and Wandosell F.
(2003)
Immortalized olfactory ensheathing glia promote axonal regeneration of rat retinal ganglion neurons.
J. Neurochem.
85
,
861-871
.
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Notes:
This paper describes the establishment of an immortalized line of olfactory bulb ensheathing glia (OEG) from rat olfactory bulbs. Immortalized lines were established by transfection of primary OEG with the plasmid pEF321-T, which expresseed the viral oncogene SV40 large T antigen. The starting primary cell culture was characterized by immunocytochemistry for OEG-specfic markers such as p75-NGFr, S100, neuroligin, vimentin and GFAP. Western blotting of p75-NGFr and GFAP was performed on the established cell lines to determine levels of these markers. The Anti-GFAP pAb was used at a concentration of 1:200 for immunocytochemistry, and at 1:1000 for Western blotting.
(0002776) |
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Products: Anti-GFAP pAb |
| 2. |
Hong, G., Liching, L., Butchbach, M., Stockinger, M. and Shan, X.
(2003)
Increased expression of the glial glutamate transporter EAAT2 modulates excitotoxicity and delays the onset but not the outcome of ALS in mice.
Hum. Mol. Genet.
12
,
2519-2532
.
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Notes:
These authors studied the glial glutamate transporter EAAT2 and its influence on amyotrophic lateral sclerosis-associated mutant mice by investigating whether supplementation of the loss of EAAT2 would delay or rescue disease progression. Protein extracts were generated from different brain regions and the spinal cord of FVB/N transgenic mice and transferred to a membrane where the Anti-GFAP pAb was used as one of the primary antibodies for immunostaining.
(0002819) |
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Products: Anti-GFAP pAb |