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Notes:
This paper describes the establishment of an immortalized line of olfactory bulb ensheathing glia (OEG) from rat olfactory bulbs. Immortalized lines were established by transfection of primary OEG with the plasmid pEF321-T, which expresseed the viral oncogene SV40 large T antigen. The starting primary cell culture was characterized by immunocytochemistry for OEG-specfic markers such as p75-NGFr, S100, neuroligin, vimentin and GFAP. Western blotting of p75-NGFr and GFAP was performed on the established cell lines to determine levels of these markers. The Anti-GFAP pAb was used at a concentration of 1:200 for immunocytochemistry, and at 1:1000 for Western blotting.
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