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| 1. |
Baumgartner, B.J. and Shine, H.D.
(1997)
Targeted transduction of CNS neurons with adenoviral vectors carrying neurotrophic factor genes confers neuroprotection that exceeds the transduced population.
J. Neurosci.
17
,
6504-6511
.
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Notes:
The GDNF Emax ImmunoAssay System was used to quantify the level of GDNF in the conditioned media of HeLa cells transduced with a recombinant adenovirus expressing GDNF. The RQ1 DNase was used to treat RNA sample to remove genomic DNA and the AMV reverse transcriptase was used for first strand cDNA synthesis.
(0001605) |
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Products: AMV Reverse Transcriptase | Anti-Human GDNF pAb | GDNF Emax® ImmunoAssay System | RQ1 RNase-Free DNase |
| 2. |
Okragly, A.J. and Haak-Frendscho, M.
(1997)
An acid-treatment method for the enhanced detection of GDNF in biological samples
Exp. Neurol.
145(2 Pt 1)
,
592-6
.
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Notes:
The levels of GDNF, NGF, and NT-3 were quantitated in various mouse tissues, including spleen, muscle, heart, lung, liver, kidney, bladder, gastrointestinal tract, brain, and serum. Additional samples screened for NGF included pig, goat, horse, chicken, rabbit, sheep, and bovine serum and rat brain. Samples were untreated or acid treated by acidification to pH <3.0 followed by neutralization to pH 7.6. In all tissue samples and most serum samples, the detectable levels of GDNF, NGF and NT-3 increased up to 35-fold. It is thought that the acidification process promotes dissociation of receptors and associated proteins making the neurotrophins more accessible. Levels of GDNF, NGF, and NT-3 were determined using the GDNF, NGF, and NT-3 Emax ImmunoAssay Systems, respectively. Please note that the coat antibody in the NGF Emax ImmunoAssay System was changed 12/2002 and may affect cross-reactivity.
(0003105) |
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Products: Anti-Human GDNF pAb | GDNF Emax® ImmunoAssay System | NGF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 3. |
Sainio, K., Suvanto, P., Davies, J., Wartiovaara, J., Wartiovaara, K., Saarma, M., Arumae, U., Meng, X., Lindahl, M., Pachnis, V., Sariola, H.
(1997)
Glial-cell-line-derived neurotrophic factor is required for bud initiation from ureteric epithelium
Development
124
,
4077-4087
.
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Notes:
The Anti-Human GDNF pAb was used to detect GDNF in homogenates of rat kidney grown in organ culture. Treatment of the cultures with chlorate dropped the GDNF levels below the detection level. The kidneys were homogenized in reducing SDS sample buffer.
(0000470) |
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Products: Anti-Human GDNF pAb |