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| 1. |
Saland, L.C., Cunningham, L.A., Su, C., Morales, M., and Gaddy, J.
(2000)
Glial cell line-derived neurotrophic factor in the rat pituitary gland
Brain Res. Bull.
52(2)
,
109-113
.
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Notes:
The Anti-Human GDNF pAb was used to detect GDNF in rat pituitaries by immunohistochemistry. To stain for GDNF alone, the rats were perfused with cold 4% paraformaldehyde, paraffin embedded, then sliced into 8µm sections. The sections were permeabilized with a brief trypsin treatment. The sections were blocked with normal donkey serum and incubated with the Anti-Human GDNF pAb (1:250 dilution) either overnight at ambient room temperature or for 48 hours at 4°C followed by colorimetric detection. For co-localization with other marker antibodies, the pituitaries were flash frozen and 10µm sections obtained. The frozen sections were fixed in 4% paraformaldehyde and permeablized with 0.3% Triton® X-100 in 5% normal goat serum. The sections were treated for 48 hours at 4°C in the presence of the Anti-Human GDNF pAb (1:250 dilution) in the goat serum/0.3% Triton® X-100 solution with accompanying antibodies. Actual levels of GDNF in the pituitaries were determined with the GDNF Emax® ImmunoAssay System.
(0000053) |
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Products: Anti-Human GDNF pAb | GDNF Emax® ImmunoAssay System |
| 2. |
Buckland, M.E., Cunningham, A.M.
(1999)
Alterations in expression of the neurotrophic factors glial cell line-derived neurotrophic factor, ciliary neurotrophic factor and brain-derived neurotrophic factor, in the target-deprived olfactory neuroepithelium
Neuroscience
90
,
333-347
.
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Notes:
The Anti-Human BDNF pAb, Anti-Rat CNTF pAb and Anti-Human GDNF pAb were used for immunohistochemical analysis of rat olfactory tissue. A lot of detail is provided for the staining. To verify the specificity of the antibodies, the antibodies were immunoabsorbed with their appropriate immunogen and all staining was abolished.
(0001370) |
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Products: Anti-Human BDNF pAb | Anti-Human GDNF pAb | Anti-Rat CNTF pAb |