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| 1. |
Barnabé-Heider F. and Miller F.D.
(2003)
Endogenously produced neurotrophins regulate survival and differentiation of cortical progenitors via distinct signaling pathways.
J. Neurosci.
23
,
5149 – 5160
.
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Notes:
This paper describes a study of the effect of endogenous neurotrophins on cortical neuron development. Cortical progenitor cells were isolated from mouse embryos and cultured. These precursor cells express both BDNF and NT-3 as well as the corresponding TrkB and TrkC receptors. BDNF and NT-3 signal via Trk receptors to activate the PI3-kinase and MEK pathways. These pathways serve distinct functions, PI3-kinase being essential for progenitor survival and MEK for the differentiation of neurons but not glial cells. The progenitor cell cultures were treated with either Anti-Human BDNF pAb or Anti-Human NT-3 pAb at 20μg/ml to block the function of the endogenous neurotrophins. Anti-ACTIVE® MAPK pAb was used to assess levels of activated MAPK by Western blotting of cell extracts.
(0002778) |
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Products: Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) | Anti-Human BDNF pAb | Anti-Human NT-3 pAb |
| 2. |
Dai, X, Lercher, L.D., Clinton, P.M., Du, Y., Livingston, D.L., Vieira, C., Yang, L., Shen, M.M. and Dreyfus, C.F.
(2003)
The trophic role of oligodendrocytes in the basal forebrain.
J. Neurosci.
23
,
5846-5853
.
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Notes:
The effect of oligodendrocytes on rat neuronal cell cultures was studied. The BDNF and NT-3 in oligodendrocyte conditioned medium (CM) were neutralized by blocking with Anti-Human BDNF and Anti-Human NT-3 pAbs. To determine whether BDNF and NT-3 are components of the CM, solutions containing authentic neurotrophins or conditioned medium solutions were preadsorbed at 4°C for 2 hours with neutralizing antibodies and then exposed to the cultures. Control groups received control IgY. Both anti-BDNF and anti-NT-3 were used at 10 μg/ml. This concentration was able to fully block BDNF-induced ChAT activity in cultures, and was able to partially block the effects of the conditioned medium for each antibody.
(0002826) |
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Products: Anti-Human BDNF pAb | Anti-Human NT-3 pAb |
| 3. |
Meier, C., Parmantier, E., Brennan, A.., Mirsky, R. and Jessen, K.R.
(1999)
Developing Schwann cells acquire the ability to survive without axons by establishing an autocrine circuit involving insulin-like growth factor, neurotrophin-3 and platelet-derived growth factor-BB.
J. Neurosci.
19
,
3847-3859
.
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Notes:
The Anti-Human NT-3 pAb was used to neutralize NT-3 activity in cultures of Schwann cells. The antibody was also used for immunocytochemistry of cultured Schwann cells and nerves of 7day old rats. The Anti-Human BDNF pAb was also used for its BDNF neutralizing activity but the data was not shown.
(0000675) |
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Products: Anti-Human BDNF pAb | Anti-Human NT-3 pAb | Chicken IgY, Control Immunoglobulin |