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| 1. |
Beattie, M.S., Harrington, A.W., Lee, R., Kim, J.Y., Boyce, S.L., Longo, F.M., Bresnahan, J.C., Hempstead, B.L. and Yoon, S.O.
(2004)
ProNGF induces p75-mediated death of oligodendrocytes following spinal cord injury.
Neuron
36
,
375–386
.
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Notes:
These authors used Western analysis to monitor levels of pro- and mature forms of BDNF and NT-3 from the center of lesions after mouse spinal cord injury. Promega Anti-Human NT-3 pAb and Anti-Human BDNF pAb were used to monitor the mature forms of these neurotrophic factors.
(0003201) |
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Products: Anti-Human BDNF pAb | Anti-Human NT-3 pAb |
| 2. |
Bianchi, L.M., Dolnick, R., Medd, A., Cohan, C.S.
(1998)
Developmental changes in growth factors released by the embryonic inner ear
Exp. Neurol.
150
,
98-106
.
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Notes:
Conditioned media from rat embryonic inner ear cultures was concentrated and analyzed by Western blotting with the Anti-Human BDNF pAb, Anti-Human NT-3 pAb and the Anti-Rat CNTF pAb. The conditioned media and various amounts of the growth factors were blotted together and developed. The Anti-BDNF pAb, Anti-NT-3 pAb and Anti-CNTF pAb were blotted with 50ng and 10ng of the respective factor along with 50ng of each of the other two factors. The Western analysis detected immunoreactivity only for NT-3 in the conditioned media. Some cross reactivity of the Anti-BDNF pAb for NT-3 and the Anti-NT-3 pAb are reported but clearly each has a preference for the respective factor. Cross reactivity to some level can be expected since both are members of the NGF family of growth factors and the proteins were denatured. As expected, no cross-reactivity with CNTF was observed since it is a different class of growth factor more closely related to cytokines. Inner ear and brain homogenates were analyzed with the BDNF Emax® ImmunoAssay System and the NT-3 Emax® ImmunoAssay System. Inner ear homogenates contained 59pg/ml of BDNF and 320pg/ml NT-3. Brain homogenates produced 440pg/ml BDNF and 436pg/ml NT-3. The BDNF Emax® ImmunoAssay did not recognized NT-3 at levels as high as 600ng/ml and the NT-3 Emax® ImmunoAssay did not recognize BDNF at levels as high as 1ng/ml.
(0000026) |
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Products: Anti-Human BDNF pAb | Anti-Human NT-3 pAb | Anti-Rat CNTF pAb | BDNF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |