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| 1. |
Leventhal, C., Rafii, S., Rafii, D., Shahar, A., and Goldman S.A
(1999)
Endothelial trophic support of neuronal production and recruitment from the adult mammalian subependyma
Mol. Cell. Neurosci.
13
,
450-64
.
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Notes:
During brain development endothelial cells may participate in neuronal recruitment, by providing neurotrophic support to new neurons. Levels of BDNF, NGF, NT-3, and NT-4 mRNAs in cultured adult human brain endothelial cells and human umbilical vascular endothelial cells were determined. The localization of BDNF within these cells was done by immunocytochemistry with the Anti-Human BDNF pAb at a 1:1000 dilution. Cells were fixed with 4% paraformaldehyde with 0.1% glutaraldehyde and permeabilized with PBS with 0.1% saponin/1% rabbit serum. The Anti-Chicken IgY, HRP Conjugate (1:200 dilution) and a DAB substrate were used to visualize staining.
(0002392) |
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Products: Anti-Chicken IgY, HRP Conjugate | Anti-Human BDNF pAb |
| 2. |
Sayers, S.T., Khan, N., Ahmed, Y., Shahid, R. and Khan, T.
(1998)
Preparation of brain-derived neurotrophic factor- and neurotrophin-3-secreting Schwann cells by infection with a retroviral vector.
J. Mol. Neurosci.
10
,
143-160
.
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Notes:
The Anti-BDNF pAb and the Anti-NT-3 pAb were used to demonstrate BDNF and NT-3 expression, respectively, in retrovirally transformed human Schwann cells. The immunocytochemistry was observed with anti-chicken IgY-FITC conjugate. The authors also used the Wizard® Plus Minipreps DNA Purification System for plasmid isolation and Promega restriction enzymes for construction of the retroviral vectors.
(0000446) |
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Products: Anti-Chicken IgY, FITC Conjugate | Anti-Human BDNF pAb | Anti-Human NT-3 pAb | Wizard® Plus Minipreps DNA Purification System |
| 3. |
Tongiorgi, E., Righi, M. and Cattaneo, A.
(1997)
Activity-dependent dendritic targeting of BDNF and TrkB mRNAs in hippocampal neurons
J. Neurosci.
17
,
9492-9505
.
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Notes:
Anti-Human BDNF pAb (1:100 dilution) was used to detect BDNF in cultured rat hippocampal neurons before and after treatment with potassium chloride and nocodazole by immunocytochemistry. The antibody was detected with the Anti-Chicken IgY Biotin Conjugate (1:200 dilution).
(0000276) |
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Products: Anti-Chicken IgY, Biotin Conjugate | Anti-Human BDNF pAb |
| 4. |
Goodman, L.J., Valverde, J., Lim, F., Geschwind, M.D., Federoff, H.J., Geller, A.I. and Hefti, F.
(1996)
Regulated release and polarized localization of brain-derived neurotrophic factor in hippocampal neurons.
Mol. Cell. Neurosci.
7
,
222-238
.
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Notes:
The anti-Human BDNF Polyclonal Antibody was used at a 1:200dilution to localize BDNF in primary cultures of rat hippocampal neurons by immunohistochemistry.
(0001574) |
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Products: Anti-Chicken IgY, HRP Conjugate | Anti-Human BDNF pAb |