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| 1. |
Koh S-W. M.
(2002)
Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
Invest. Ophthalmol. Vis. Sci.
43
,
2887-2896
.
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Notes:
These authors studied release of CNTF by corneal endothelial (CE) cells that survived oxidative stress. Bovine corneoscleral explant cultures were treated with H2O2 to induce oxidative stress. CE cells were collected from the explants and homogenized, and CNTF levels were determined by Western blotting with Anti-Rat CNTF pAb.
(0002781) |
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Products: Anti-Rat CNTF pAb |
| 2. |
Bianchi, L.M., Dolnick, R., Medd, A., Cohan, C.S.
(1998)
Developmental changes in growth factors released by the embryonic inner ear
Exp. Neurol.
150
,
98-106
.
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Notes:
Conditioned media from rat embryonic inner ear cultures was concentrated and analyzed by Western blotting with the Anti-Human BDNF pAb, Anti-Human NT-3 pAb and the Anti-Rat CNTF pAb. The conditioned media and various amounts of the growth factors were blotted together and developed. The Anti-BDNF pAb, Anti-NT-3 pAb and Anti-CNTF pAb were blotted with 50ng and 10ng of the respective factor along with 50ng of each of the other two factors. The Western analysis detected immunoreactivity only for NT-3 in the conditioned media. Some cross reactivity of the Anti-BDNF pAb for NT-3 and the Anti-NT-3 pAb are reported but clearly each has a preference for the respective factor. Cross reactivity to some level can be expected since both are members of the NGF family of growth factors and the proteins were denatured. As expected, no cross-reactivity with CNTF was observed since it is a different class of growth factor more closely related to cytokines. Inner ear and brain homogenates were analyzed with the BDNF Emax® ImmunoAssay System and the NT-3 Emax® ImmunoAssay System. Inner ear homogenates contained 59pg/ml of BDNF and 320pg/ml NT-3. Brain homogenates produced 440pg/ml BDNF and 436pg/ml NT-3. The BDNF Emax® ImmunoAssay did not recognized NT-3 at levels as high as 600ng/ml and the NT-3 Emax® ImmunoAssay did not recognize BDNF at levels as high as 1ng/ml.
(0000026) |
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Products: Anti-Human BDNF pAb | Anti-Human NT-3 pAb | Anti-Rat CNTF pAb | BDNF Emax® ImmunoAssay System | NT-3 Emax® ImmunoAssay System |
| 3. |
Liu, C., Peng, M., Laties, A.M., and Wen, R.
(1998)
Preconditioning with bright light evokes a protective response against light damage in the rat retina.
J. Neurosci.
18
,
1337-1344
.
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Notes:
Exposure to bright light induces photoreceptor degeneration in the retina and over time upregulates the expression of neurotrophic factors that protect photoreceptors. Endogenous basic fibroblast growth factor and cilliary neurotrophic factor appear to play roles in photoreceptor protection while activation of MAP kinase aids in photoreceptor survival. The level of CNTF after exposure to bright light in rat retinas was monitored by Western blot analysis using the Anti-Rat CNTFpAb. The level of MAP Kinase activation was determined by Western blot analysis to detect with dually phosphorylated forms of MAP kinase with the Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY)
(0002374) |
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Products: Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) | Anti-Rat CNTF pAb |