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| 1. |
Thoby-Brisson, M., Cauli, B., Champagnat, J., Fortin, G. and Katz, D.M.
(2003)
Expression of functional tyrosine kinase B receptors by rhythmically active respiratory neurons in the pre-Bötzinger complex of neonatal mice.
J. Neurosci.
23
,
7685–7689
.
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Notes:
The anti-TrkB In polyclonal antibody was used to immunohistochemically stain brains of 1 to 4 day old mice. The researchers used a 1:100 dilution of the antibody on 20-30μm thick brain sections. Sections were stained in three steps with biotin labeled anti-chicken antibody, a streptavidin-HRP conjugate, and an Alexa Fluor 488 tyramide dye that is preferentially reduced by HRP (Molecular Probes). Details of the entire procedure are provided. BDNF and NGF were also used in in vitro neuron studies to examine neuron respiratory frequency. For these experiments the researchers added 100ng/ml of each neurotrophic factor to disected pre-Bötzinger complexes (PBCs).
(0002764) |
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Products: Anti-TrkB In pAb | mNGF, 7S | rhBDNF |
| 2. |
Spears, N., Molinek, M.D., Robinson, L.L.L., Fulton, N., Cameron, H., Shimoda, K., Telfer, E.E., Anderson, R.A., and Price, D.J.
(2003)
The role of neurotrophin receptors in female germ-cell survival in mouse and human.
Development
130
,
5481 - 5491
.
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Notes:
TrkB was shown to be important in the survival of germ cells during follicle formation in the ovaries. Immunohistochemical analysis of mouse ovaries showed the presence of TrkB. TrkB was found in similar patterns in germ cells both before and after follicle formation, but TrkB protein was more abundant in P0 oocytes than in E16 oogonia. IHC was performed on ovaries fixed in Bouin's fixative then embedded in wax. Anti-TrkB In pAb was used at a 1:10 dilution.
(0002829) |
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Products: Anti-TrkB In pAb |
| 3. |
Balkowiec, A., Kunze, D.L., and Katz, D.M.
(2000)
Brain-derived neurotrophic factor acutely inhibits AMPA-mediated currents in developing sensory relay neurons.
J. Neurosci.
20
,
1904-1911
.
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Notes:
The authors set out to determine the role of BDNF in modulating glutamatergic transmission between primary afferents and second-order neurons. Sections of rat brain, specifically the nucleus tractus solitarius, and primary cell cultures from the nucleus tractus solitarius were stained ny immunohistochemistry and immunocytochemistry with Promega's Anti-TrkB pAb to detect cells expressing the TrkB receptor.
(0002376) |
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Products: Anti-TrkB In pAb |