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| 1. |
Leitlein, J., Aulwurm, S., Waltereit, R., Naumann, U., Wagenknecht, B., Garten, W., Weller, M., and Platten, M.
(2001)
Processing of immunosuppressive pro-TGF-beta 1,2 by human glioblastoma cells involves cytoplasmic and secreted furin-like proteases.
J. Immunol.
166
,
7238-7243
.
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Notes:
Inhibitors of furin-like protease activity interfere with processing and secretion of TGFβ1 and β2 from cells. Levels of TGFβ1 released into spent culture medium of SV40 large T-Ag immortalized fetal human astrocytic cell line Sv-FHAS and the human glioma cell lines LN-18 and T98G were determined by Western blot analysis using the Anti-TGFβ1 pAb (1:2000 dilution). Due to the low protein concentrations, the spent medium was concentrated with the Centriplus centrifugal filter device YM-3 (Millipore) prior to SDS-PAGE.
(0002455) |
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Products: Anti-TGFβ1 pAb |
| 2. |
Bouzahzah, B., Fu, M., Iavarone, A., Factor, V.M., Thorgeirsson, S.S., and Pestell, R.G.
(2000)
Transforming growth factor-beta1 recruits histone deacetylase 1 to a p130 repressor complex in transgenic mice in vivo.
Cancer Res.
60
,
4531-37
.
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Notes:
The authors find that TGF-β1 may regulate interactions between histone deacetylase and the p130 protein, shedding more light on the involvement of chromatin remodeling proteins in oncogenesis. Transgenic mice overexpressing hepatic TGF-β1 were used to study the relationship between levels of TGFβ1 and the histone deacetylase/p130 complex. To monitor TGFβ1 levels, hepatic tissue extracts were subjected to Western blot analysis using the Anti-TGFβ1 pAb.
(0002456) |
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Products: Anti-TGFβ1 pAb |
| 3. |
Mozes, M.M., Hodics, T. and Kopp. J.B.
(1999)
Isoform specificity of commercially-available anti-TGF-beta antibodies.
J. Immunol. Methods
225
,
87-93
.
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Notes:
The Anti-TGFβ1 pAb was used in Western blots and compared with three other anti-TGF antibodies. When the Western results were analyzed by band densitometry, Anti-TGFβ1 pAb displayed 0.3% cross-reactivity to reduced TGFβ2 and TGFβ3. The researchers determined that Western blots performed with the Anti-TGFβ1 pAb were sensitive to 50pg of human recombinant TGFβ1.
(0002766) |
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Products: Anti-TGFβ1 pAb |
| 4. |
Wang, J., Friedman, E.A.
(1998)
Short-chain fatty acids induce cell cycle inhibitors in colonocytes
Gastroenterology
114
,
940-946.
.
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Notes:
The Anti-TGFβ1 pAb (1:5,000 dilution) was used to probe U4 human colon carcinoma cell extracts by Western blotting following treatment of the cells with short chain fatty acids.
(0000202) |
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Products: Anti-TGFβ1 pAb |