• NanoLuc™
  • Performances de NanoLuc™
  • Conférences en ligne
  • Devenez Testeur
  • Vecteurs
  • Réactifs

La technologie NanoLuc™ :

Plus petite, plus lumineuse et d'une extrême sensibilité

 
  • Extrême luminosité :

    • La sensibilité optimisée permet de détecter des événements physiologiques plus pertinents
    • Sur cellules, le signal est de 80 à 240 fois supérieur à la firefly (luc2)
  • Petite taille sans équivalent (19kD) :

    • Nettement plus petit que la Firefly ou la Renilla, ou que les GFP
    • Permet des applications dans lesquelles les tailles des gènes ou des protéines sont restrictives, telles que la libération de particules virales et les protéines de fusion
  • Cinétique des signaux lumineux :

    • Convient généralement à toutes les capacités de traitement de laboratoire
    • S'adapte facilement à l'automatisation de laboratoire (par ex., HTS)
  • Haute stabilité physique :

    • Robuste dans les conditions expérimentales (température, pH, etc.)
    • Réduction des faux-positifs dans le criblage de banque de composés
  • Grande polyvalence :

    • Format standard avec lyse cellulaire
    • Sécrétion dans le milieu sans lyse cellulaire
    • Jouez sur la stabilité de votre protéine grâce à une séquence de dégradation (-PEST)

“For the past 25 years, firefly luciferase has served as the standard in bioluminescent reporter technology. Through series of incremental advancements in both reporter structure and reagent formulation, this native bioluminescent chemistry has been transformed into a reliable and highly sensitive indicator of genetic regulation. To further augment the capabilities of bioluminescent detection, we have engineered a novel luciferase and corresponding assay chemistry.

This new reporter technology can supply ample luminescence in challenging biological systems where transgene expression is low, sample size is restricted, or transgene length is constrained. Furthermore, the favorable physical properties and bright luminescence are particularly suitable for emerging methods in cellular analysis, such as luminescence imaging and energy transfer”.

Keith Wood, R&D director

Les performances de NanoLuc™

NanoLuc™ Luciferase is offered in a collection of 12 different vector configurations to best suit the intended use. These products offer :
  • Nluc gene sequence codon optimized and cleansed of most transcription factor binding sites, promoter modules, splice sites, and other regulatory sequences.
  • Standard, destabilized, or secreted forms of luciferase available to match the most appropriate assay format.
  • pGL4-based backbone for easy sequence transfer from existing plasmids
  • Use a standard plasmid with Multiple Cloning Site (MCS) to clone promoters or Minimal Promoter-containing plasmid to clone response elements
  • Hygromycin marker available for cell line creation
  • CMV controls available in standard (intracellular) and secreted format
  • NF-kB response element plasmid with destabilized NlucP for study of the NF-kB pathway

CRE response in HEK293 cells

CRE_Response_In_HEK293_Cells

Genetic reporter constructs where made using the indicated luciferase under control of the cAMP response element (CRE) and transfected into HEK293 cells. Following induction with forskolin, the luciferase expression was detected with the Nano-Glo™ or ONE-Glo® Luciferase Assay Systems on a GloMax® 96 Microplate Luminometer. NanoLuc™ Luciferase demonstrates brighter signals than either standard or destabilized firefly luciferase. The PEST destabilization domain increases the induction ratio for both luciferases and provides maximal response when used with NanoLuc. In all cases the EC50 for the induction remains relatively unchanged.
 

HEK293 cells / CRE response element

10588MA

The CRE-responsive expression of secreted NanoLuc™ Luciferase (secNluc) was measured either in cells induced with forskolin or in DMSO-control over a period of 6 hours. A media change was performed at t=0. The secreted format allows the measurement of reporter activation kinetics without destroying the sample. Signals were measured with a GloMax® 96 Microplate Luminometer.

Nano-Glo™ Assay Reagent

Nano-Glo™ Luciferase Assay System has been developed to provide optimal activity with the NanoLuc™ Luciferase. This assay reagent offers :
  • Add-Mix-read simplicity with integral lysis components
  • Glow signal kinetics with an approximately 2 hour signal half-life
  • Liquid substrate allows easy reagent preparation
  • Minimal autoluminescense
  • Room temperature stability through one work day
  • Four sizes availabe : 10mL | 10x10mL | 100mL | 10x100mL
 

Nano-Glo Detection Reagent


10588MA
  • Furimazine substrate: novel coelenterazine analog (ATP independent)
  • t½ > 2 hours at room temperature
  • Very low autoluminescence
  • Stable at room temperature for several hours


Produit     Cdtm. Référence Prix
pNL1.1[Nluc] Vector Carte Séquence 20μg N1001 413 €
pNL1.2[NlucP] Vector Carte Séquence 20μg N1011 413 €
pNL1.3[secNluc] Vector Carte Séquence 20μg N1021 413 €
pNL3.1[Nluc/minP] Vector Carte Séquence 20μg N1031 413 €
pNL3.2[NlucP/minP] Vector Carte Séquence 20μg N1041 413 €
pNL3.3[secNluc/minP] Vector Carte Séquence 20μg N1051 413 €
pNL2.1[Nluc/Hygro] Vector Carte Séquence 20μg N1061 413 €
pNL2.2[NlucP/Hygro] Vector Carte Séquence 20μg N1071 413 €
pNL2.3[secNluc/Hygro] Vector Carte Séquence 20μg N1081 413 €
pNL1.1.CMV[Nluc/CMV] Vector Carte Séquence 20μg N1091 413 €
pNL1.3.CMV[secNluc/CMV] Vector Carte Séquence 20μg N1101 413 €
pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Carte Séquence 20μg N1111 413 €


Produit Cdtm. Référence Prix
Nano-Glo™ Luciferase Assay Protocol 10ml N1110 84 €
Nano-Glo™ Luciferase Assay Protocol 100ml N1120 652 €
Nano-Glo™ Luciferase Assay Protocol 10x10ml N1130 634 €
Nano-Glo™ Luciferase Assay Protocol 10x100ml N1150 sur demande