Promega Corporation


New Products

Please note that this website is for our most recently released products. For a complete list of Promega's new products, click here

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DNA and RNA Analysis |Protein Expression, Purification & Analysis | Cellular Analysis| Automation & Robots|Genetic Identity | Stem Cells


DNA and RNA Analysis

Improve your qPCR reactions

GoTaq qPCR Master Mix is a new qPCR reagent that contains a new DNA binding dye that often exhibits greater fluorescence than SYBR® Green. Combined with the GoTaq Hot Start and an optimized buffer, GoTaq qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity.

Take the GoTaq qPCR Challenge


Transfection quality plasmid DNA in as little as ten minutes
The PureYield Plasmid Miniprep System isolates high quality plasmid DNA suitable for a broad range of downstream applications including transfection, in vitro expression, sequencing and cloning. The system allows direct purification of plasmid from 0.6ml of bacterial culture or purification from a cell pellet from as much as 3ml of culture. Elution volumes can be as low as 30ul.
More information | Product catalogue | Request a free sample

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 Protein Expression, purification & Analysis

Quick and Easy Protein Purification with HaloTag
The HaloTag Protein Purification System has the following advantages:

+ Superior protein yield and purity

+ Enhanced solubility of proteins expressed in E. coli compared to

   His6Tag, GST and MBP

+ One simple buffer for all purification steps

More information | View an animation of this method


New proteases for Mass Spectrometry:  Chymotrypsin & Endoproteinase Lys-C

Endoproteinase Lys-C is a sequencing grade serine protease that hydrolyzes specifically at the carboxyl side of Lys.  The enzyme is optimal in the pH range of 7.0–9.0 and may be used alone or in combination with other proteases for peptide mapping applications or protein identification by mass spec. It is suitable for digestion reactions in-solution or in-gel.

Endoproteinase Lys-C catalogue page

Chymotrypsin is a highly-purified serine endopeptidase derived from bovine pancreas that preferentially hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met.  The enzyme is optimal in the pH range of 7.0–9.0 and may be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

Chymotrypsin catalogue page


Simplify your Mass Spectra by using Immobilized Trypsin
Promega trypsin now comes in a convenient immobilized format.  Immobilized Trypsin provides a fast and convenient method for digesting purified or complex proteins over a wide range of concentrations. With the spin column format, digested peptides are easily separated from the Immobilized Trypsin, reducing enzyme interference during analysis.

More information | Catalogue page


ECL Western Blotting Substrate

The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of HRP.

More information

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CellULAR Analysis

Measure ADP levels direct with this accurate, sensitive, and universal kinase assay

ADP-Glo Kinase Assay is a luminescent assay that measures ADP formation from a kinase reaction. ADP is converted into ATP, which is converted into light by Ultra-Glo luciferase. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

More information | Request a sample


Response Elements in pGL4 Luciferase Reporter Vectors
Optimized luciferase reporter vectors offers both firefly and Renilla luciferases in a variety of configurations. Now also available with response elements to study pathways of interest.  Response elements currently available are CRE, NFAT-RE, GAL4UAS, NF-kB-RE, SRE, SRF-RE, and MMTV.
Go to the pGL4 Vector Selector | Catalogue page


Measure deconjugating enzyme activity

The DUB-Glo™ Protease Assay (DUB/SENP/NEDP) is a homogeneous, bioluminescent assay that measures the activity of numerous deconjugating enzymes including deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases. These proteases reverse the protein modification by ubiquitin and ubiquitin-like proteins (Ubl proteins) and thus are integral components in the complex mechanisms of posttranslational protein regulation in eukaryotes.

More information


New ADME Assay

The UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity. UGTs are an important family of Phase II metabolizing enzymes in humans.  Complete assay systems are available for UGT1A1 and UGT2B7.

More information | Catalogue page | Request a sample

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Automation & Robots

Automated DNA & RNA purification for clinical labs

The new Maxwell 16 MdX Instrument provides easy-to-use, consistent and reliable automated nucleic acid extraction of 1 to 16 samples, bar-code sample tracking, a touch-screen in-terface and UV decontamination. Available in Low Elution Volume and Standard Elution Volume.  Like the regular Maxwell 16, it can purify DNA or RNA from up to 16 samples in 30-45 minutes.

Visit the Maxwell 16 website

Request a demo | Browse the applications database


Automated Total Nucleic Acid Purification

Viral total nucleic acid extraction is a critical step in infectious disease research or molecular diagnostics. The Maxwell® 16 Viral Total Nucleic Acid Purification Kit is used with the Maxwell® 16 Instrument configured with the low elution volume (LEV) hardware.  The total nucleic acid purification procedure is an easy method for efficient, automated purification of viral total nucleic acid from up to 16 samples. The low elution volume of 50μl results in more concentrated purified nucleic acid for downstream applications such as quantitative PCR (qPCR) or quantitative RTPCR (qRT-PCR). The Maxwell® 16 Instrument purifies high-quality viral total nucleic acid from 100μl, 200μl or 300μl of plasma or serum.

 
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Genetic identity

Cleaner profiles from contaminated samples

The PowerPlex® 16 HS System system allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin) and provides all materials necessary for amplification, including hot-start Taq DNA polymerase.  The buffering system has been optimized for high inhibitor tolerance.

More information

 


PowerPlex European Systems

The PowerPlex® ESX and ESI Systems meet the new ENFSI recommendation for DNA profile sharing across Europe. They are based on five-color technology and allow co-amplification and detection of D3S1358, D8S1179, D18S51, D21S11, FGA, THO1, vWA, D2S441, D10S1248, D22S1045, D1S1656, D12S391, D2S1358, D16S539, D19S433, SE33 and Amelogenin.  PowerPlex® ESX Systems contains five New ENFSI-recommended loci as mini STRs, plus standard European loci.  PowerPlex® ESI Systems contain standard European loci as mini STRs, plus the five new ENFSI-recommended loci.

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Stem Cells
Authenticate your cell lines
The StemElite™ System provides the reagents required for successful and simple identification and authentication of human cell lines and detection of intra-species cell line cross-contamination (specifically mouse). The StemElite™ System uses short tandem repeat (STR) analysis of specific loci in the human genome through co-amplification and three-color detection of ten loci (nine STR loci and Amelogenin for gender identification) and one loci specific to mouse. All eleven STR regions are amplified simultaneously in a single tube.

More information

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