General

Training Support Programme

If you are holding a course on molecular biology we can offer you a 30% discount off the products to be used in the course.

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Promega Points™ Frequent Buyer Programme

Every time you order a product we automatically include Promega Points for you to collect and redeem for valuable merchandise*. Items currently in the programme include: Laboratory items, gift vouchers and personal items.

- 1GB iPod® Shuffle is now only 250 points
- 2GB iPod® Shuffle is now only 380 points

- Eluator™ has been added, eliminate swinging bucket rotors for nucleic acid purification

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Nucleic Acid Purification

Increase Productivity with Vacuum based RNA purification

- RNA from nearly any sample type
- Mini and midi column format RNA purification
- Ready for sensitive downstream assays
- Throughput from single columns to 96 well formats

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Purify genomic DNA from almost any source

- Purify DNA in about 20 minutes
- Isolate amplifiable genomic DNA from 5 x 106 cells, 209mg of tissue or 1.2cm of mouse tails

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Enhance your productivity with plasmid purification systems

- Isolate 20ug of plasmid DNA from 1-10ml of bacterial culture
- Isolate transfection-quality DNA in less than one-hour

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Efficient, high-capacity DNA Purification from reactions or gels

- purification is complete in as little as 15mins
- As great as 95% recovery in elution volumes as little as 15ul
- Recovered DNA is ready for automated sequencing, cloning, labeling, restriction enzyme digestion

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Genomics

Essential tools for cDNA construction

Promega can supply you with all the necessary components for cDNA construction. In addition we have listed all the special offers that are available in this area.

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Nucleic acid purification - Boost your efficiency with these time-saving products:

Transfection quality plasmid purification with 20% off the list price

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Wizard® SV DNA and SV RNA purification systems as much as 60% less expensive than other brands

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Request your nucleic acid purification free sample

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Get a pump for free and switch to the vacuum format for improved performance

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Drug Discovery & Cell Analysis

Caspase Assays simplify apoptosis detection and are up to 50-100 times more sensitive than competitive caspase technologies. Now, detect caspase-3/7, -8, -9, -2 and -6 activities in multiwell plates using less sample for faster throughput.

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CytoTox-Glo™ Cytotoxicity Assay

The most sensitive assay available to quantify cytotoxicity due to loss of membrane integrity.

Measure "dead-cell" protease biomarker leaked from cytoplasm into media.

Loss of membrane integrity is a key indicator of cytotoxicity to determine the mechanism of cell death

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The Ultimate in Reporter Gene Assays

ONE-Glo™ Luciferase Assay Reagent provides unequaled, robust performance while eliminating many of the handling inconveniences that may be experienced when using reporter assays, especially in an HTS environment.

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Functional Proteomics

Cell-Free Expression System
TNT T7 Quick Translation/Transcription System

The TNT® T7 Quick Coupled Transcription/Translation System is available for transcription and translation of genes cloned downstream from the T7 promoters. To use this system, 0.2–2.0μg of circular plasmid DNA containing a T7 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TNT® Quick Master Mix and incubated in a 50μl reaction volume for 60–90 minutes at 30°C.

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TNT SP6 High Yield Protein Expression System

The TNT® SP6 High-Yield Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.

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S30 T7 High-Yield Protein Expression System

Designed to express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for translation. This results in greater stability and enhanced expression of target proteins.

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