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ADME Assays
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Protease Assays
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Forensic
DNA
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Genomics
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Transfection quality plasmid DNA in as
little as ten minutes
The PureYield™
Plasmid Miniprep System isolates high
quality plasmid DNA suitable for a broad
range of downstream applications including
transfection, in vitro expression,
sequencing and cloning. The system allows
direct purification of plasmid from 0.6ml of
bacterial culture or purification from a
cell pellet from as much as 3ml of culture.
Elution volumes can be as low as 30ul.
Product catalogue |
Request your free sample
here
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No more swinging bucket rotors for nucleic acid
purification
The Eluator™ eliminates the requirement for a centrifuge
with a swinging bucket rotor for nucleic acid
purification, simplifying and speeding purification
protocols. Eluators are available for:
Plasmid Midipreps Purify plasmid DNA in 30mins -
More information
Plasmid Maxipreps Purify plasmid DNA in 60mins -
More information
RNA Midipreps -
More information
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Hot Start Taq Polymerase
GoTaq® Hot Start Polymerase contains the high
performance GoTaq® DNA polymerase bound to a proprietary
antibody that blocks polymerase activity.
More information
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Product catalogue |
Request your free sample
here
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Introducing Personal Automation™ from
Promega
The Maxwell® 16 System offers automated and truly
walk-away purification that saves time and labour by eliminating
reagent preparation, pipetting, and centrifugation steps.
Comprised of a pre-programmed instrument and pre-filled reagent
cartridges, the system processes up to 16 samples in about 30
minutes. Simply add samples or lysate directly to the reagent
cartridges and press "start" to purify DNA, RNA, or
protein with consistent yield and purity.
At the touch of a button, you achieve a new level of confidence
in your results
More information and request a
demonstration |
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Functional
Proteomics
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Insect cell-free protein expression
The TNT® T7 Insect Cell
Extract Protein Expression System is a
convenient, quick, single-tube, coupled
transcription/translation system for the cell-free
expression of proteins. Protein synthesis reactions are
initiated by the addition of a DNA template, eliminating
the need for the time-consuming process of in vitro RNA
synthesis.
More information | Free
sample
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Express up to 500ug of protein in only 1 hour!
The E. coli S30 T7 High-Yield Protein Expression System
is designed to express up to 500ug/ml of protein in one
hour from plasmid vectors containing a T7 promoter and a
ribosome binding site. The system provides an extract
that contains T7 RNA polymerase for transcription and
all other necessary components in the system are
optimized for translation.
More information | Free
sample
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Trypsin enhancer
ProteaseMax™ is designed to enhance the performance of
trypsin or chymotrypsin in any digestion reaction
performed with the objective of characterizing the
products by mass spectrometry or liquid chromatography.
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Drug Discovery and Cell Analysis |
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Rapidly develop your reporter assays
The GloResponseTM Luciferase Reporter Cell Lines
contain optimized, state-of-the-art luciferase reporter
technology integrated into a cell line. This allows the rapid
development of a reporter assay based on the pathway of interest
regulating the luciferase gene.
More information |
Authenticate your cell lines
The Cell ID™ System provides the reagents required for
successful and simple identification and authentication of human
cell lines and detection of intra-species cell line
cross-contamination. The Cell ID™ System uses short tandem
repeat (STR) analysis of specific loci in the human genome
through co-amplification and three-color detection of ten loci
(nine STR loci and Amelogenin for gender identification),
including D21S11, TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539,
D7S820, D13S317 and D5S818. All ten loci are amplified
simultaneously in a single tube.
More information |
Bioluminescent cell-based assays to measure all three
proteasome protease activities
The Proteasome-Glo™ Assays are homogeneous, luminescent assays
that individually measure the chymotrypsin-like, trypsin-like
and caspase-like protease activity associated with the
proteasome complex in cultured cells. Addition of the
Proteasome-Glo™ Cell-Based Reagent in an "add-mix-measure"
format results in proteasome cleavage of the substrate and rapid
generation of a luminescent signal produced by the luciferase
reaction.
More information |
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Bioluminescent reporter vectors
The pGL4 Luciferase Reporter Vectors are the next generation of
reporter gene vectors optimized for expression in mammalian
cells. Numerous configurations of pGL4 Vectors are available.
The newest vector - pGL4.32[luc2P/NF-κB-RE/Hygro] vector can be
used in transient transfections or to generate a stable cell
line for studying NF-κB induction.
More information
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Multi-mode detection system
The GloMax®-Multi Detection System is the
next-generation integrated solution from Promega. While
maintaining state-of-the-art luminescence performance, the
GloMax® line now extends into fluorescence and
absorbance applications. By combining assay reagents with a
high-performance detection instrument and simple, preprogrammed
software from a single source, it's now easier than ever to
start capturing real results.
More information
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Bioluminescence imaging technology
VivoGlo™ substrates are a line of high-quality luminescent and
pro-luminescent substrates specifically designed for in vivo
bioluminescent imaging applications. The VivoGlo™ products are suitable
for established imaging applications in addition to opening up new areas
of investigation, such as in vivo apoptosis studies.
More information |
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Measuring Kinase Activity
The Kinase-Glo® Max Luminescent Kinase Assay is a
homogeneous, high-throughput screening method of measuring kinase
activity by quantifying the amount of ATP remaining in solution
following a kinase reaction.
Product Catalogue |
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Glutathione shown to be a key biomarker of oxidative stress
Versatile: Measure reduced glutathione and/or total glutathione
levels from cells, tissue, blood...
Fast and Easy to Use: Measure GSH levels directly from cell
culture wells; no deproteinization step required.
Excellent Sensitivity: Able to measure GSH levels from as few as
several hundred cells. Easily scalable to 384-well plates.
Robust: No interference by oxidized GSH or reducing agents.
Proven Luminescent Technology: Assay powered by Ultra-Glo™
Luciferase. No interference by fluorescent compounds.
More information and apply for a free sample |
Product catalogue |
Fluorescent Cell Viability Assay
The CellTiter-Fluor™ Cell Viability Assay is a non-lytic,
single-reagent-addition fluorescence assay that measures the relative
number of viable cells in a population.
Product Catalogue |
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Bioluminescent Cytotoxicity Assay
The CytoTox-Glo™ Assay is a luminescent cytotoxicity assay that measures
the relative number of dead cells in cell populations. The CytoTox-Glo™
Assay measures the extracellular activity of a distinct intracellular
protease activity (dead-cell protease) when the protease is released
from membrane-compromised cells.
More information and apply for
a free sample |
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ADME Assays |
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Screen drugs and new chemical
entities for their capacity to modulate P-glycoprotein ATPase
activity
The Pgp-Glo™ Assay Systems provide the necessary reagents for
performing luminescent P-glycoprotein (Pgp) ATPase assays.
Product
catalogue |
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Escape the limits of fluorescence
The P450-Glo™ Screening
Systems provide a complete set of
reagents for performing luminescent cytochrome P450 assays.
Systems are available for CYP1A2, CYP2C9 CYP3A4,
CYP2C19 and CYP2D6.
More
information |
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Protease Assays |
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Achieve greater sensitivity with the
new luminescent DPPIV-Glo™ Protease Assay
This system offers excellent Z’ factor values. Simply use in an
add, mix measure format.
Product catalogue |
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Up
to 1000x greater sensitivity than
fluorescence.
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent
assay that measures calpain 1 (u)
and 2 (m) activities.
Product catalogue |
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Forensic DNA |
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DNA from Forensic Casework Samples
The DNA IQ™ Casework Sample Kit for the Maxwell® 16 System is
designed specifically for the optimal extraction of DNA from forensic
casework samples. These samples may include: blood stains, semen stains,
hairs, cigarette butts, tissue samples, and trace or "touch" DNA samples
regularly encountered in forensic DNA analysis.
Product Catalogue |
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There is more to see
If there is DNA to detect, Plexor® HY will detect it. A
single Plexor® HY assay will provide consistent DNA detection
down to 6.4pg. If amplifiable DNA is present Plexor® HY will quantitate
it, eliminating any unnecessary PCR amplifications.
Product Catalogue |
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