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New Products
Please note that this website is for our most
recently released products. For a complete list of Promega's new
products, click here
Quick links:
Amplification
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DNA and RNA Purification |
In Vitro Protein Expression |
Cellular Analysis
| ADME Assays
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Protease Assays
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Amplification |
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Hot Start Taq Polymerase
GoTaq® Hot Start Polymerase contains the high
performance GoTaq® DNA polymerase bound to a
proprietary antibody that blocks polymerase activity
until you start your reactions. GoTaq®
Hot Start is available as a standard Mg-adjustable DNA
polymerase or as a Green or Colourless master mix.
More information
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Product catalogue |
Request a free sample |
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DNA and RNA Purification |
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Transfection quality plasmid DNA in as
little as ten minutes |
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The PureYield™
Plasmid Miniprep System isolates high
quality plasmid DNA suitable for a broad
range of downstream applications including transfection, in vitro expression,
sequencing and cloning. The system allows
direct purification of plasmid from 0.6ml of
bacterial culture or purification from a
cell pellet from as much as 3ml of culture.
Elution volumes can be as low as 30ul.
More information |
Product catalogue |
Request a free sample |
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Several new
options for
Personal Automation™ of DNA purification
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Maxwell®
16
Cell LEV DNA Purification Kit:
This low elution volume kit can purify DNA
from 10 - 104 cells and will work
with a number of biological fluids. Product catalogue |
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Maxwell®
16 FFPE Tissue LEV DNA Purification Kit:
This
low elution volume kit purifies DNA from
formalin-fixed paraffin-embedded tissue
sections without the need to use organic
solvents. Product catalogue
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Maxwell® 16 Mouse Tail DNA Purification Kit:
This kit is optimized for DNA purification from mouse
tails, ear punches and other tissue.
Product catalogue |
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Maxwell® 16 Flexi
Method Firmware:
Are you trying to do something out of
the ordinary?
This firmware gives you the ability to optimize multiple
instrument parameters to tailor instrument operation to
your unique needs. It’s Personal Automation™ just the
way you want it.
More information |
Product catalogue |
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In
Vitro Protein Expression
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Insect cell-free protein expression
The TNT® T7 Insect Cell
Extract Protein Expression System is a
convenient, quick, single-tube, coupled
transcription/translation system for the cell-free
expression of proteins. Protein synthesis reactions are
initiated by the addition of a DNA template, eliminating
the need for the time-consuming process of in vitro RNA
synthesis.
Optimal expression (up to
75μg/ml of functional protein) is achieved when
the gene encoding the protein of interest is in a companion
vector (pF25A
or pF25K T7 Flexi® Vector)
containing 5' and 4' untranslated region (UTR) sequences
that enhance translation efficiency.
More information | Free
sample
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Express up to 500ug of
protein in only 1 hour!
The E. coli S30 T7 High-Yield
Protein Expression System is designed to
express up to 500ug/ml of protein in one hour from
plasmid vectors containing a T7 promoter and a ribosome
binding site. The system provides an extract that
contains T7 RNA polymerase for transcription and all
other necessary components in the system are optimized
for translation.
More information | Free
sample
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CellULAR Analysis |
Authenticate your cell lines
The StemElite™ System provides the reagents required for
successful and simple identification and authentication of human
cell lines and detection of intra-species cell line
cross-contamination (specifically mouse). The StemElite™ System uses short tandem
repeat (STR) analysis of specific loci in the human genome
through co-amplification and three-color detection of ten loci
(nine STR loci and Amelogenin for gender identification) and one
loci specific to mouse. All eleven STR regions are amplified
simultaneously in a single tube.
More information |
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In vivo bioluminescent
reporter vectors
New pGL4 Luciferase Reporter Vectors
pGL4.50(luc2/CMV/Hygro) and
pGL4.51(luc2/CMV/Neo) provide constitutive selectable
luciferase expression and are for
use in in vivo
bioluminescence imaging applications.
More information
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ADME Assays |
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Escape the limits of fluorescence with our new P450 Substrates
Five new isoform-specific P450 luminogenic substrates
(Luciferin-3A7, -4A, -4F2/3, -4F12, and
-2J/4F12) can be used with new stand-alone
Luciferin Detection Reagents to make
do-it-yourself ADME assays. We've also developed
Luciferin-MultiCYP, which targets
at least 21 P450 isoforms (see left) and is useful for measuring net CYP
activity in a mixed population of P450s. And
Luciferin-NAT2
is an excellent substrate for N-acetyltransferase 2 (NAT2) with
virtually no cross-reactivity to NAT1.
More information |
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Protease Assays |
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New Live-Cell Biosensor for
GPCR Studies
The GloSensor™ cAMP Assay
provides a new way to measure cAMP levels in cells. The
assay uses a genetically modified form of firefly luciferase
containing a cAMP-binding protein moeity. Binding of cAMP causes
a conformational change that leads to increased light output.
The assay is ideal for GPCRs that signal through cAMP.
More information
Easy evaluation of protease
function
The
Protease-Glo Assay, our
second assay that uses GloSensor™ technology, allows you to
probe protease function. You design and clone your
protease recognition sequence into our
pGloSensor-10F vector and the luminescent assay
evaluates the level of cleavage.
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HaloTag Technology |
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Get started with HaloTag today!
The HaloTag®
Technology provides new options to enable the understanding of protein
function in a biochemical and cellular environment. Our
HaloTag® Cloning Starter System
allows you to create your HaloTag fusion gene and put it
under a series of CMV promoter deletions to get just the level of
expression you desire.
More information |
Product catalogue
Couple the starter system
with smaller (15ml)
volume ligands and you are good to go!
The
HaloTag®
TMR, diAcFAM, Coumarin, Oregon Green, and Alexa Fluor Fluorescent
Ligands are for labeling live and fixed cells using the
HaloTag technology. The HaloTag®
Biotin and PEG-Biotin Ligands are affinity ligands used to
capture HaloTag protein fusion constructs using the strong biotin-streptavidin
interaction.
Product catalogue
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