Essential tools for cDNA construction

Quick Links: Isolate DNA  | Transcribe to cDNA | Add cDNA Primers | Pull the pieces together | Ensure Clean results

Promega can supply you with all the necessary components for cDNA construction. In addition we have listed all the special offers that are available in this area.


 

Isolate RNA PureYield™ RNA Midiprep Systems Get pure RNA without qPCR-detectable gDNA. PureYield™ RNA systems eliminate the gDNA without using DNase, even before the total RNA is purified.

Special Offers: Try a free sample1 Get a vacuum pump for free2

More information: View the catalogue page Component Listing Technical Manual

SV Total RNA Isolation System
The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.
 

Size	Catalogue No.
50 preps	Z3100
250 preps	Z3105

Please enquire about special prices for bulk orders

Special Offers: As much as 41% less expensive than other brands3 Get a vacuum pump for free2 Try a free sample1

More Information: View the catalogue page Technical Manual Component Listing

RQ1 RNase-free DNase
Other purification systems may produce enough clean RNA for end-point RT-PCR, but is it clean enough for quantitative RT-PCR? Use RQ1 RNase-free DNase to remove genomic DNA from any total RNA preparation to get cleaner starting material.

Size	Concentration	Catalogue No.
1,000u	1u/μl	M6101

Please enquire about special prices for bulk orders

More Information: View the catalogue page

Product Information Component Listing

Transcribe to cDNA M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant The premier enzyme for generating longer cDNAs even through regions of RNA secondary structure. Reaction temperature may be optimized from 37 - 55°C.

Size	Catalogue No.
2,500u	M3681
10,000u	M3682
50,000u	M3683

Please enquire about special prices for bulk orders

More Information: View the catalogue page

Product Information Component Listing

ImProm-II™ Reverse Transcriptase
The enzyme is specifically tested for maximum cDNA synthesis from a 7.5kb RNA template and has been shown to transcribe messages as long as 8.9kb. Reaction temperature can be optimized from 37 - 55°C.

Size	Catalogue No.
10 reactions	A3801
100 reactions	A3802
500 reactions	A3803

Please enquire about special prices for bulk orders

More Information: View the catalogue page

Component Listing Technical Manual

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Add cDNA primers Random and Oligo(dT)15 Primers The random hexanucleotide and oligo(dT)15 primers are specifically tested and qualified for cDNA synthesis prior to end-point RT-PCR.

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Pull the pieces together dNTP Mix A premixed solution of dATP, dCTP, dGTP and dTTP each at a concentration of 10mM in water.

dNTP Sets
Separate tubes of dATP, dCTP, dGTP and dTTP each supplied at 100mM in water.

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Ensure Clean Results RNasin® Plus RNase Inhibitor Protect your RNA from unwanted RNase contamination. RNasin® Plus RNase Inhibitor-the most trusted name in RNase Inhibition-is backed by more than 10,000 peer-reviewed citations. RNasin Inhibitors are perfectly compatible with quantitative and end-point RT-PCR.

Size	Concentration	Catalogue No.
2,500u	40u/μl	N2611
10,000u	40u/μl	N2615
Please enquire about special prices for bulk orders

¹ One sample per customer, available while stocks last.
² Conditions apply, please view this webpage for more details.
³ Conditions apply, please view this webpage for more details.