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Abstract for Genomic DNA Purification from Cigarette Butts and Buccal Swabs
Using the DNA IQ™ System
DNA is isolated from cigarette butts and cigarette butt paper using the DNA IQ™
System in the presence and absence of a proteinase K digestion. In addition
a protocol to isolate DNA from buccal swabs in the absence of a centrifugation step
is developed. These methods simplify the pretreatment steps for these samples and demonstrate
that centrifugation of the lysate following initial incubation may not be required for
successful DNA isolation. Eliminating the centrifugation steps make complete processing
of these sample types on automated workstations possible.
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