Cell Line Authentication
An estimated 15–20% of the time,
cells used in experiments have been misidentified or cross-contaminated with another cell line. This creates
problems for data quality and can waste precious time, resources and reagents in the laboratory. STR
analysis has become the preferred technology for rapidly authenticating cell lines. Promega offers several products
to help you with cell line authentication including: Cell ID™ System, StemElite™ System and the
PowerPlex® 1.2 System.
Featured Product
Stem Elite™ ID System
The StemElite™ ID System provides the reagents required for successful and simple identification and authentication of human cell lines and detection of intra-species cell line cross-contamination. The StemElite™ ID System uses short tandem repeat (STR) analysis of specific loci in the human genome through co-amplification and three-color detection of ten loci (nine STR loci and Amelogenin for gender identification). Additionally, the StemElite™ ID System incorporates a sensitive marker that specifically detects the presence of DNA from mouse (Mus musculus). All eleven loci are amplified simultaneously in a single tube.
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Cell ID™ System
The Cell ID™ System allows coamplification and three-color detection of nine human loci. The system offers the convenience of room-temperature reaction assembly by including a hot-start Taq DNA polymerase system in a 5X enzyme mix. This allows a simple, one-step addition of Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer necessary for DNA amplification.
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