Questions & Answers

Use this Product Selector to learn how Promega's complete portfolio of RNAi products provides the best choices when RNAi is critical to your research.

About How dd RNAi Functions

About dd RNAi Expression Cassettes

About dd RNAi Expression Vectors

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Online Tools

siRNA Target Designer
Design oligonucleotides for use with Promega's systems which can be used to produce siRNAs.

 

Related Products

GeneClip™ U1 Hairpin Cloning System

siSTRIKE™ U6 Hairpin Cloning Systems

SilentGene™-2 U6 Hairpin Cloning System

CodeBreaker™ siRNA Transfection Reagent

T7 RiboMAX™ Express RNAi System

Products

The siSTRIKE™ U6 Hairpin Cloning System—hMGFP includes a vector for production of siRNAs. This vector contains an internal fluorescent marker, the Monster Green™ Fluorescent Protein (hMGFP). The presence of the hMGFP gene enables transfection efficiency to be determined easily and facilitates selection of transfected cells by fluorescence-activated cell sorting (FACS^®).

Figure 1. siSTRIKE™ -hMGFP monitors delivery of shRNA expressing constructs without effecting their ability for gene silencing. (Enlarge Figure)

The psiCHECK™ Vectors provide a quantitative and rapid approach for optimization of RNA interference (RNAi). A target gene of interest can be cloned into the multiple cloning region located downstream of the Renilla translational stop codon. Initiation of RNAi toward the target gene results in cleavage and subsequent degradation of the fusion mRNA including the Renilla luciferase RNA sequence. This RNA degradation results in decreased Renilla luciferase activity.

Figure 2. Mechanism of action of the psiCHECK™ Vectors. ( Enlarge Figure)

RNA Interference Products

History and Mechanism of RNA Interference

Technically Speaking: RNAi

 

Promega Articles

NEW! The most up-to-date Promega Protocols & Applications Guide is now available! Read the RNAi Chapter online.

Request a brochure describing our RNA interference systems in detail.

Suppression of Caspase-3 Expression Using the psiSTRIKE™ hMGFP Vector

Everything You Need for RNAi: The Use of Bioluminescent Reporter Genes for RNAi Optimization

dd RNAi Made Easy: DNA-Directed RNA Interference: Hairpin Cloning and Expression Made Easy

Silencing Genes with a Hairpin: Introducing siLentGene™-2 U6 Hairpin Cloning Systems

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References

RNAi Mechanism References

dd RNAi References
(Viral and Plasmid-based RNAi)

 

Reviews and Applications

Genc, S. et al. (2004) RNA interference in neuroscience. Brain Res Mol Brain Res. 132, 260-70.

Nesterova, M. and Cho-Chung, Y-S (2004) Killing the Messenger: Antisense DNA and siRNA. Curr Drug Targets. 5, 683-9.

Borkhardt, A. and Heidenreich, O. (2004) RNA interference as a potential tool in the treatment of leukaemia. Expert Opin Biol Ther. 4, 1921-9.

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	Applications
Product	Screening of siRNA Targets	Transient RNAi	Stable RNAi	Transfection of dsRNA oligos	Verification of Transfection Efficiency
psiCHECK™ Vectors	ⓘ
siSTRIKE™ U6 Hairpin Cloning System	ⓘ	ⓘ	ⓘ
siLentGene™-2 U6 Hairpin Cloning System	ⓘ	ⓘ	ⓘ
T7 RiboMAX™ Express RNAi System	ⓘ	ⓘ
CodeBreaker™ siRNA Transfection Reagent				ⓘ
siSTRIKE™ U6 Hairpin Cloning System-hMGFP	ⓘ	ⓘ			ⓘ
GeneClip™ U1 Hairpin Cloning Systems	ⓘ	ⓘ	ⓘ
GeneClip™ U1 Hairpin Cloning System-hMGFP	ⓘ	ⓘ			ⓘ