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PROTEIN EXPRESSION & ANALYSIS

MagneHis™ Protein Purification System

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The MagneHis Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis Ni-Particles) are used to isolate polyhistidine- or HQ-tagged proteins directly from a crude lysate of bacterial, insect or mammalian cells, or from culture medium using either a manual or automated procedure.

Bacterial, insect or mammalian cells containing a polyhistidine- or HQ-tagged protein are lysed directly in their culture medium using the FastBreak™ Cell Lysis Reagent, 10X and MagneHis Ni-Particles are added to the lysate. The polyhistidine- or HQ-tagged protein binds to the Ni-Particles in a matter of minutes. The unbound protein is washed away and the target protein is recovered by elution with imidazole.

• Shorten the wait for results
  No long incubations with lysozyme; no centrifugation or vacuum required
• Achieve maximum binding capacity
  Up to 1mg of polyhistidine-tagged protein per 1ml of MagneHis Ni-Particles
• One step lysis
  Lysis buffer is added directly to the cells in culture medium
• Pick your platform
  Product format provides versatility for either manual or automated platforms

MagneHis™ Protein Purification System Technical Manual (TM060)

MagneHis™ Protein Purficiation System - online catalog information