HisLink™ Protein
Purification Resin
HisLink™ Protein Purification Resin is designed to efficiently
capture and purify polyhistidine-tagged (His-tag) fusion proteins
expressed in bacterial cells.
High Binding Capacity Improves Yield
HisLink™ Protein Purification Resin provides higher protein-binding capacity than the
leading competitors.
Skip Lysate Clearing and Save Time
In batch format, HisLink™ Protein Purification Resin may be easily
separated from cell lysates without filtration (or centrifugation). Protein may be purified from large volumes of lysate without prior clearing of
insoluble cellular debris (Figure 1).
Figure 1. Purification of 6X His-tagged fusion protein
using HisLink™ Protein Purification Resin in a column and batch format
with both cleared and crude lysates.
Lane 1. Protein marker.
Lane 2. Bacterial cell lysate expressing a 61kD 6X His-tagged
protein.
Lane 3. Flow through of lysate (column, cleared).
Lane 4. Flow through of lysate (batch, cleared).
Lane 5. Flow through of lysate (batch, crude).
Lane 6. Eluted 6X His-tagged protein (column, cleared).
Lane 7. Eluted 6X His-tagged protein (batch, cleared).
Lane 8. Eluted 6X His-tagged protein (batch, crude).
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HisLink™ Protein Purification Resin
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