Imaging, Immunological Detection & Protein Labeling

Promega has state-of-the-art protein detection and labeling products for applications such as in vivo imaging, protein trafficking, and in vitro detection of expressed proteins.

Featured Protein Detection Products

New! ECL Western Blotting Substrate

  • Ready to use
  • Sensitive detection
  • No optimization required
View catalog listing See the protocol
multicolor live and fixed cell imaging

HaloTag® Fluorescent Ligands:
Cellular Imaging

Study protein trafficking in live cells, perform multicolor live and fixed-cell imaging...all with a single genetic construct.

Imaging with HaloTag ...

in vivo imaging with luciferase

In vivo Imaging

The image to the right illustrates use of the VivoGlo™ Caspase-3/7 Substrate to detect apoptotic cells by in vivo imaging using a repTOP™ mouse expressing luciferase. Image courtesy of TOP srl, Milan.

Read about in vivo imaging with bioluminescence in the following articles:

Identification of Apoptotic Cells in Living Reporter Mice Using Modified Luciferin Imaging with luciferase in whole animals In vivo evaluation of regulatory sequences Bioluminescence imaging of TRAIL-induced apoptosis Selective killing of hypoxia-inducible factor-1-active cells

Colorimetric and Chemiluminescent Labeling Systems

Eliminate the need for [35S]methionine with the Transcend™ Systems.

See a quick protocol View catalog listing

Fluorescent Labeling

With the FluoroTect™ Green System, you can incorporate fluorescently labeled lysines during cell-free expression. Labeled proteins are detected directly in the gel.

See a protocol View catalog listing Request Protein Labeling Systems Brochure

Immunological Detection: Dot Blots and Western Blots

Alkaline Phosphatase-Conjugated Antibodies HRP-Conjugated Antibodies Western Blue® Stabilized Substrate for Alkaline Phosphatase TMB Stabilized Substrate for Horseradish Peroxidase
anti-phospho, anti-active antibodies

Anti-ACTIVE® Antibodies

Specific for the active forms of caspase-3, JNK, CaM KII, MAPK and 38, these antibodies can be used for Western blotting (except for Anti-ACTIVE® Caspase-3), immunocytochemistry and immunohistochemistry.

Click on image to enlarge.


Learn more about Anti-ACTIVE® Antibodies...

Emax® ImmunoAssay Systems

Emax® ImmunoAssay Systems are available to detect:

BDNF (brain-derived neurotrophic factor)

GDNF (glial cell-line-derived neurotrophic factor)

NGF (nerve growth factor)

NT-3 (neurotrophin-3)

transforming growth factors β1 (TGFβ1) and β2 (TGFβ2)

Learn more about the Emax® Systems...