Cell Imaging with HaloTag® Technology
Multiple Applications for Protein Functional Analysis–More Versatile than GFP
With HaloTag® Technology you can perform multicolor live- and fixed-cell imaging experiments using a single genetic construct. The technology is based on the formation of a covalent bond between a protein fusion tag and synthetic ligands, and is designed to enable understanding of protein function in a cellular and biochemical environment.
Use the HaloTag Flexi Vector Selector to find the HaloTag® Vector you need.
Featured Cell Imaging Applications
Temporal/Spatial Separation of Proteins in Live Cells
Fluorophores attached to HaloTag® ligands with different abilities to cross the cell membrane are used to regionally label a protein of interest.
Multiplex Live-Cell Imaging
The HaloTag Protein is not intrinsically fluorescent (unlike GFP). Therefore, the choice of fluorescent labels, including secondary and tertiary fluorophores, can be made after creating the HaloTag fusion protein. This gives you more flexibility in choice of fluorophores than GFP.
Label Proteins in Fixed Cells--Combine with ICC for 2-color Imaging
HaloTag ligands retain their fluorescent properties after fixation, enabling multiplexing immunocytochemistry experiments.
Cell-to-Gel Analysis of Proteins
The HaloTag protein-ligand interaction is stable and highly specific. The fluorescently labeled HaloTag protein can be boiled with SDS-PAGE sample buffer and resolved on a gel without loss of fluorescent signal.