Protein Purification

ProTEV Protease
An improved 50kDa version of the Nla protease from Tobacco Etch Virus (TEV), ProTEV Protease has been engineered for more stability than native TEV protease, providing longer enzymatic stability. Used to cleave affinity tags from fusion proteins after protein purification, ProTEV is highly site-specific and is also easily removed after cleavage using metal affinity resins.

Maxwell^® 16 Polyhistidine Protein Purification Kit
Automated purification of polyhistidine-tagged protein from bacterial cultures and other sample types including mammalian and insect cells.

MagneHis™ Protein
Purification System
Simple, rapid, magnetic-based purification of polyhistidine- or HQ-tagged expressed proteins.

MagneGST™ Protein Purification System
High yields with the speed and efficiency of a magnetic-based purification system for GST-tagged proteins.

Protein Analysis

Trypsin Gold,
Mass Spectrometry Grade
Use in conjunction with mass spectrometry to confirm the identity of protein interaction partners.

Protein Expression

Single Step (KRX) Competent Cells
Directly transform your vector into the Single Step (KRX) Competent Cells and be ready for protein expression.

TNT SP6 High-Yield Protein Expression System
Convenient cell-free expression with yields up to 100µg/ml in only two hours.

Cell-Free Expression System Selector
Find the correct Cell-Free Protein Expression System for your situation.

Promega TNT^® Systems
Use cell-free protein expression systems to generate functional protein in only 90 minutes directly from DNA templates.

The Flexi^® Vector System
Easily and efficiently transfer protein-coding regions between Flexi vectors designed for multiple expression formats (e.g., bacterial, mammalian, cell-free expression). For specific ORF content in Flexi format (FlexClones), click here.

Protein Labeling

HaloTag^® Technology
Multiple and interchangeable fluorophores for cell imaging.

FluoroTect™ Green_Lys in vitro Translation Labeling System
Fluorescently labeled lysines are incorporated during cell-free expression of proteins. No requirement for transfer to nitrocellulose/nylon. Labeled proteins detected directly in gel.

Resources

TNT Product Selector

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Coupled Transcription/Translation System for Cell-Free Functional Proteomics

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Promega Articles

The Role of Cell-Free Rabbit Reticulocyte Expression Systems in Functional Proteomics (1.3 MB PDF)

The Single Step (KRX) Competent Cells: Efficient Cloning and High Protein Yields

Functional Protein Production in the TNT^® SP6 High-Yield Protein Expression System

The Flexi^® Vector Systems: The Easy Way to Clone

TNT^® SP6 High-Yield Protein Expression System: More Protein from a Coupled Transcription/Translation System

More articles...